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Article

DADS downregulates the Rac1-ROCK1/PAK1-LIMK1-ADF/cofilin signaling pathway, inhibiting cell migration and invasion

  • Authors:
    • Yujuan Zhou
    • Jian Su
    • Ling Shi
    • Qianjin Liao
    • Qi Su
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Cancer Cellular and Molecular Pathology of Hunan Provincial University, Cancer Research Institute, University of South China, Hengyang, Hunan 421001, P.R. China
  • Pages: 605-612
    |
    Published online on: December 6, 2012
       https://doi.org/10.3892/or.2012.2168
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Abstract

The aim of this study was to explore the molecular mechanisms of the diallyl disulfide (DADS)-mediated downregulation of LIM kinase-1 (LIMK1) and the consequent inhibition of the migration and invasion of human colorectal cancer cells. RNA interference technology was used to establish stable LIMK1-miRNA/SW480 cell lines. The effects of DADS and LIMK1 RNA interference on the migration and invasion of SW480 cells were observed by scratch wound healing assay and Transwell migration assay. The effects of DADS on signaling molecules of the Rac1-Rho kinase (ROCK)1/p21-activated kinase (PAK)1-LIM kinase (LIMK)1-actin depolymerizing factor (ADF)/cofilin pathway in SW480 cells were examined by RT-PCR and western blot analysis. The healing and migration rate of the SW480 cells was significantly reduced and the cell penetrating ability was significantly suppressed (P<0.05) following treatment with DADS (45 mg/l). The immunohistochemistry and western blot analysis results showed that DADS significantly downregulated LIMK1 protein expression and suppressed LIMK1 protein phosphorylation. Furthermore, the RT-PCR and western blot analysis results revealed that DADS suppressed Rac1, ROCK1, PAK1, LIMK1 and destrin mRNA and protein expression, as well as the protein phosphorylation of LIMK1 and cofilin 1. The data demonstrate that LIMK1 expression positively correlates with the SW480 cell migration and invasion ability. DADS downregulates the Rac1-ROCK1/PAK1-LIMK1-ADF/cofilin signaling pathway, suppressing SW480 cell migration and invasion.
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Copy and paste a formatted citation
Spandidos Publications style
Zhou Y, Su J, Shi L, Liao Q and Su Q: DADS downregulates the Rac1-ROCK1/PAK1-LIMK1-ADF/cofilin signaling pathway, inhibiting cell migration and invasion. Oncol Rep 29: 605-612, 2013.
APA
Zhou, Y., Su, J., Shi, L., Liao, Q., & Su, Q. (2013). DADS downregulates the Rac1-ROCK1/PAK1-LIMK1-ADF/cofilin signaling pathway, inhibiting cell migration and invasion. Oncology Reports, 29, 605-612. https://doi.org/10.3892/or.2012.2168
MLA
Zhou, Y., Su, J., Shi, L., Liao, Q., Su, Q."DADS downregulates the Rac1-ROCK1/PAK1-LIMK1-ADF/cofilin signaling pathway, inhibiting cell migration and invasion". Oncology Reports 29.2 (2013): 605-612.
Chicago
Zhou, Y., Su, J., Shi, L., Liao, Q., Su, Q."DADS downregulates the Rac1-ROCK1/PAK1-LIMK1-ADF/cofilin signaling pathway, inhibiting cell migration and invasion". Oncology Reports 29, no. 2 (2013): 605-612. https://doi.org/10.3892/or.2012.2168
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou Y, Su J, Shi L, Liao Q and Su Q: DADS downregulates the Rac1-ROCK1/PAK1-LIMK1-ADF/cofilin signaling pathway, inhibiting cell migration and invasion. Oncol Rep 29: 605-612, 2013.
APA
Zhou, Y., Su, J., Shi, L., Liao, Q., & Su, Q. (2013). DADS downregulates the Rac1-ROCK1/PAK1-LIMK1-ADF/cofilin signaling pathway, inhibiting cell migration and invasion. Oncology Reports, 29, 605-612. https://doi.org/10.3892/or.2012.2168
MLA
Zhou, Y., Su, J., Shi, L., Liao, Q., Su, Q."DADS downregulates the Rac1-ROCK1/PAK1-LIMK1-ADF/cofilin signaling pathway, inhibiting cell migration and invasion". Oncology Reports 29.2 (2013): 605-612.
Chicago
Zhou, Y., Su, J., Shi, L., Liao, Q., Su, Q."DADS downregulates the Rac1-ROCK1/PAK1-LIMK1-ADF/cofilin signaling pathway, inhibiting cell migration and invasion". Oncology Reports 29, no. 2 (2013): 605-612. https://doi.org/10.3892/or.2012.2168
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