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Article

Knockdown of integrin α3β1 expression induces proliferation and migration of non-small cell lung cancer cells

  • Authors:
    • Hyun Jae Yoon
    • Young-Rak Cho
    • Ji-Hye Joo
    • Dong-Wan Seo
  • View Affiliations / Copyright

    Affiliations: Quality Management Department, Hugel Inc., Chuncheon 200-821, Republic of Korea, College of Pharmacy, Dankook University, Cheonan 330-714, Republic of Korea
  • Pages: 662-668
    |
    Published online on: December 6, 2012
       https://doi.org/10.3892/or.2012.2169
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Abstract

Integrin α3β1 is expressed on many types of cancer cells and can regulate tumor growth and progression. In the present study, we examined the roles and molecular mechanism of integrin α3β1 in modulating cell proliferation and migration of p53-deficient non-small cell lung cancer (NSCLC) cells. Reduced expression of integrin α3 by RNA silencing clearly induces cell proliferation and migration in H1299 cells, compared with those in control cells. Enhanced proliferation in integrin α3-silenced cells is mediated by upregulation and nuclear localization of cyclin-dependent kinases, and these effects require the activation of Akt and ERK as evidenced by treatment with LY294002 and PD98059, respectively. Furthermore, suppression of integrin α3 expression induces the expression of nuclear factor-κB and Bcl-2 as well as epidermal growth factor receptor, which are positively correlated with cell proliferation and survival. In contrast, increase in cell migration of integrin α3-silenced cells is found to be independent of Akt or ERK signaling pathways. Collectively, these findings suggest that integrin α3β1 plays pivotal roles in regulating cell proliferation and migration that enhance the invasive type of p53-deficient NSCLC cells.
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Copy and paste a formatted citation
Spandidos Publications style
Yoon HJ, Cho Y, Joo J and Seo D: Knockdown of integrin α3β1 expression induces proliferation and migration of non-small cell lung cancer cells. Oncol Rep 29: 662-668, 2013.
APA
Yoon, H.J., Cho, Y., Joo, J., & Seo, D. (2013). Knockdown of integrin α3β1 expression induces proliferation and migration of non-small cell lung cancer cells. Oncology Reports, 29, 662-668. https://doi.org/10.3892/or.2012.2169
MLA
Yoon, H. J., Cho, Y., Joo, J., Seo, D."Knockdown of integrin α3β1 expression induces proliferation and migration of non-small cell lung cancer cells". Oncology Reports 29.2 (2013): 662-668.
Chicago
Yoon, H. J., Cho, Y., Joo, J., Seo, D."Knockdown of integrin α3β1 expression induces proliferation and migration of non-small cell lung cancer cells". Oncology Reports 29, no. 2 (2013): 662-668. https://doi.org/10.3892/or.2012.2169
Copy and paste a formatted citation
x
Spandidos Publications style
Yoon HJ, Cho Y, Joo J and Seo D: Knockdown of integrin α3β1 expression induces proliferation and migration of non-small cell lung cancer cells. Oncol Rep 29: 662-668, 2013.
APA
Yoon, H.J., Cho, Y., Joo, J., & Seo, D. (2013). Knockdown of integrin α3β1 expression induces proliferation and migration of non-small cell lung cancer cells. Oncology Reports, 29, 662-668. https://doi.org/10.3892/or.2012.2169
MLA
Yoon, H. J., Cho, Y., Joo, J., Seo, D."Knockdown of integrin α3β1 expression induces proliferation and migration of non-small cell lung cancer cells". Oncology Reports 29.2 (2013): 662-668.
Chicago
Yoon, H. J., Cho, Y., Joo, J., Seo, D."Knockdown of integrin α3β1 expression induces proliferation and migration of non-small cell lung cancer cells". Oncology Reports 29, no. 2 (2013): 662-668. https://doi.org/10.3892/or.2012.2169
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