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Article

Hypoxia-mediated histone acetylation and expression of N-myc transcription factor dictate aggressiveness of neuroblastoma cells

  • Authors:
    • Jitka Poljaková
    • Tomáš Groh
    • Žaneta Omana Gudino
    • Jan Hraběta
    • Lucie Bořek‑Dohalská
    • René Kizek
    • Helena Doktorová
    • Tomáš Eckschlager
    • Marie Stiborová
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry, Faculty of Science, Charles University, 128 40 Prague 2, Czech Republic, Department of Pediatric Hematology and Oncology, 2nd Medical School, Charles University and University Hospital Motol, 150 06 Prague 5, Czech Republic, Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, 613 00 Brno, Czech Republic
  • Pages: 1928-1934
    |
    Published online on: January 27, 2014
       https://doi.org/10.3892/or.2014.2999
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Abstract

Cells of solid malignancies generally adapt to entire lack of oxygen. Hypoxia induces the expression of several genes, which allows the cells to survive. For DNA transcription, it is necessary that DNA structure is loosened. In addition to structural characteristics of DNA, its epigenetic alterations influence a proper DNA transcription. Since histones play a key role in epigenetics, changes in expression levels of acetylated histones H3 and H4 as well as of hypoxia-inducible factor-1α (HIF-1α) in human neuroblastoma cell lines cultivated under standard or hypoxic conditions (1% O2) were investigated. Moreover, the effect of hypoxia on the expression of two transcription factors, c-Myc and N-myc, was studied. Hypoxic stress increased levels of acetylated histones H3 and H4 in UKF-NB-3 and UKF-NB-4 neuroblastoma cells with N-myc amplification, whereas almost no changes in acetylation of these histones were found in an SK-N-AS neuroblastoma cell line, the line with diploid N-myc status. An increase in histone H4 acetylation caused by hypoxia in UKF-NB-3 and UKF-NB-4 corresponds to increased levels of N-myc transcription factor in these cells.
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1 

Maris JM and Matthay KK: Molecular biology of neuroblastoma. J Clin Oncol. 17:2264–2279. 1999.PubMed/NCBI

2 

Morgenstern BZ, Krivoshik AP, Rodriguez V and Anderson PM: Wilms’ tumor and neuroblastoma. Acta Paediatr Suppl. 93:78–85. 2004.

3 

Brodeur GM: Neuroblastoma: biological insights into a clinical enigma. Nat Rev Cancer. 3:203–216. 2003. View Article : Google Scholar : PubMed/NCBI

4 

Tang XX, Zhao H, Kung B, Kim DY, Hicks SL, Cohn SL, Cheung NK, Seeger RC, Evans AE and Ikegaki N: The MYCN enigma: significance of MYCN expression in neuroblastoma. Cancer Res. 66:2826–2833. 2006.PubMed/NCBI

5 

Lutz W and Schwab M: In vivo regulation of single copy and amplified N-myc in human neuroblastoma cells. Oncogene. 15:303–315. 1997. View Article : Google Scholar : PubMed/NCBI

6 

Westermann F, Muth D, Benner A, Bauer T, Henrich KO, Oberthuer A, Brors B, Beissbarth T, Vandesompele J, Pattyn F, Hero B, König R, Fischer M and Schwab M: Distinct transcriptional MYCN/c-MYC activities are associated with spontaneous regression or malignant progression in neuroblastomas. Genome Biol. 9:R1502008. View Article : Google Scholar : PubMed/NCBI

7 

Fulda S, Lutz W, Schwab M and Debatin KM: MycN sensitizes neuroblastoma cells for drug-induced apoptosis. Oncogene. 18:1479–1486. 1999. View Article : Google Scholar : PubMed/NCBI

8 

Porro A, Haber M, Diolaiti D, Iraci N, Henderson M, Gherardi S, Valli E, Munoz MA, Xue C, Flemming C, Schwab M, Wong JH, Marshall GM, Della Valle G, Norris MD and Perini G: Direct and coordinate regulation of ATP-binding cassette transporter genes by Myc factors generates specific transcription signatures that significantly affect the chemoresistance phenotype of cancer cells. J Biol Chem. 285:19532–19543. 2010. View Article : Google Scholar

9 

Harris AL: Hypoxia - a key regulatory factor in tumour growth. Nat Rev Cancer. 2:38–47. 2002. View Article : Google Scholar : PubMed/NCBI

10 

Höckel M and Vaupel P: Tumor hypoxia: definitions and current clinical, biologic, and molecular aspects. J Natl Cancer Inst. 93:266–276. 2001.PubMed/NCBI

11 

Furchert SE, Lanvers-Kaminsky C, Juürgens H, Jung M, Loidl A and Frühwald MC: Inhibitors of histone deacetylases as potential therapeutic tools for high-risk embryonal tumors of the nervous system of childhood. Int J Cancer. 120:1787–1794. 2007. View Article : Google Scholar : PubMed/NCBI

12 

Decock A, Ongenaert M, Vandesompele J and Speleman F: Neuroblastoma epigenetics: from candidate gene approaches to genome-wide screenings. Epigenetics. 6:962–970. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Santini V, Gozzini A and Ferrari G: Histone deacetylase inhibitors: molecular and biological activity as a premise to clinical application. Curr Drug Metab. 8:383–393. 2007. View Article : Google Scholar : PubMed/NCBI

14 

Portela A and Esteller M: Epigenetic modifications and human disease. Nat Biotechnol. 28:1057–1068. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Kouzarides T: Chromatin modifications and their function. Cell. 128:693–705. 2007. View Article : Google Scholar : PubMed/NCBI

16 

Watson JA, Watson CJ, McCrohan AM, Woodfine K, Tosetto M, McDaid J, Gallagher E, Betts D, Baugh J, O’Sullivan J, Murrell A, Watson RW and McCann A: Generation of an epigenetic signature by chronic hypoxia in prostate cells. Hum Mol Genet. 8:3594–3604. 2009. View Article : Google Scholar : PubMed/NCBI

17 

Frank SR, Schroeder M, Fernandez P, Taubert S and Amati B: Binding of c-Myc to chromatin mediates mitogen-induced acetylation of histone H4 and gene activation. Genes Dev. 15:2069–2082. 2001. View Article : Google Scholar : PubMed/NCBI

18 

Frank SR, Parisi T, Taubert S, Fernandez P, Fuchs M, Chan HM, Livingston DM and Amati B: MYC recruits the TIP60 histone acetyltransferase complex to chromatin. EMBO Rep. 4:575–580. 2003. View Article : Google Scholar : PubMed/NCBI

19 

Li Q and Costa M: c-Myc mediates a hypoxia-induced decrease in acetylated histone H4. Biochimie. 91:1307–1310. 2009. View Article : Google Scholar : PubMed/NCBI

20 

Stiborová M, Martínek V, Rýdlová H, Hodek P and Frei E: Sudan I is a potential carcinogen for humans: evidence for its metabolic activation and detoxication by human recombinant cytochrome P450 1A1 and liver microsomes. Cancer Res. 62:5678–5684. 2002.

21 

Poljaková J, Eckschlager T, Kizek R, Frei E and Stiborová M: Electrochemical determination of enzymes metabolizing ellipticine in thyroid cancer cells - a tool to explain the mechanism of ellipticine toxicity to these cells. Int J Electrochem Sci. 8:1573–1585. 2013.

22 

Gut P and Verdin E: The nexus of chromatin regulation and intermediary metabolism. Nature. 502:489–498. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Buechner J and Einvik C: N-myc and noncoding RNAs in neuroblastoma. Mol Cancer Res. 10:1243–1253. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Huang R, Cheung NK, Vider J, Cheung IY, Gerald WL, Tickoo SK, Holland EC and Blasberg RG: MYCN and MYC regulate tumor proliferation and tumorigenesis directly through BMI1 in human neuroblastomas. FASEB J. 25:4138–4149. 2011. View Article : Google Scholar : PubMed/NCBI

25 

Liu XH, Yu EZ, Li YY and Kagan E: HIF-1α has an anti-apoptotic effect in human airway epithelium that is mediated via Mcl-1 gene expression. J Cell Biochem. 97:755–765. 2006.

26 

Wang GL, Jiang BH, Rue EA and Semenza GL: Hypoxia-inducible factor 1 is a basic-helix-loop-helix-PAS heterodimer regulated by cellular O2 tension. Proc Natl Acad Sci USA. 92:5510–5514. 1995. View Article : Google Scholar : PubMed/NCBI

27 

Adamski J, Price A, Dive C and Makin G: Hypoxia-induced cytotoxic drug resistance in osteosarcoma is independent of HIF-1Alpha. PloS One. 8:e653042013. View Article : Google Scholar : PubMed/NCBI

28 

Zhong H, De Marzo AM, Laughner E, Lim M, Hilton DA, Zagzag D, Buechler P, Isaacs WB, Semenza GL and Simons JW: Overexpression of hypoxia inducible factor 1α in common human cancers and their metastases. Cancer Res. 59:5830–5835. 1999.

29 

Talks KL, Turley H, Gatter KC, Maxwell PH, Pugh CW, Ratcliffe PJ and Harris AL: The expression and distribution of the hypoxia-inducible factors HIF-1α and HIF-2α in normal human tissues, cancers, and tumor associated macrophages. Am J Pathol. 157:411–421. 2000.

30 

Kwon HJ, Kim MS, Kim MJ, Nakajima H and Kim KW: Histone deacetylase inhibitor FK228 inhibits tumor angiogenesis. Int J Cancer. 97:290–296. 2002. View Article : Google Scholar : PubMed/NCBI

31 

Hubbi ME, Kshitiz, Gilkes DM, Rey S, Wong CC, Luo W, Kim DH, Dang CV, Levchenko A and Semenza GL: A nontranscriptional role for HIF-1α as a direct inhibitor of DNA replication. Sci Signal. 6:ra102013.

32 

Bedrnicek J, Vicha A, Jarosova M, Holzerova M, Cinatl J Jr, Michaelis M, Cinatl J and Eckschlager T: Characterization of drug-resistant neuroblastoma cell lines by comparative genomic hybridization. Neoplasma. 52:415–419. 2005.PubMed/NCBI

33 

Poljakova J, Hrebackova J, Dvorakova M, Moserova M, Eckschlager T, Hrabeta J, Göttlicherova M, Kopejtkova B, Frei E, Kizek R and Stiborova M: Anticancer agent ellipticine combined with histone deacetylase inhibitors, valproic acid and trichostatin A, is an effective DNA damage strategy in human neuroblastoma. Neuro Endocrinol Lett. 32(Suppl 1): 101–116. 2011.

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Copy and paste a formatted citation
Spandidos Publications style
Poljaková J, Groh T, Gudino ŽO, Hraběta J, Bořek‑Dohalská L, Kizek R, Doktorová H, Eckschlager T and Stiborová M: Hypoxia-mediated histone acetylation and expression of N-myc transcription factor dictate aggressiveness of neuroblastoma cells. Oncol Rep 31: 1928-1934, 2014.
APA
Poljaková, J., Groh, T., Gudino, Ž.O., Hraběta, J., Bořek‑Dohalská, L., Kizek, R. ... Stiborová, M. (2014). Hypoxia-mediated histone acetylation and expression of N-myc transcription factor dictate aggressiveness of neuroblastoma cells. Oncology Reports, 31, 1928-1934. https://doi.org/10.3892/or.2014.2999
MLA
Poljaková, J., Groh, T., Gudino, Ž. O., Hraběta, J., Bořek‑Dohalská, L., Kizek, R., Doktorová, H., Eckschlager, T., Stiborová, M."Hypoxia-mediated histone acetylation and expression of N-myc transcription factor dictate aggressiveness of neuroblastoma cells". Oncology Reports 31.4 (2014): 1928-1934.
Chicago
Poljaková, J., Groh, T., Gudino, Ž. O., Hraběta, J., Bořek‑Dohalská, L., Kizek, R., Doktorová, H., Eckschlager, T., Stiborová, M."Hypoxia-mediated histone acetylation and expression of N-myc transcription factor dictate aggressiveness of neuroblastoma cells". Oncology Reports 31, no. 4 (2014): 1928-1934. https://doi.org/10.3892/or.2014.2999
Copy and paste a formatted citation
x
Spandidos Publications style
Poljaková J, Groh T, Gudino ŽO, Hraběta J, Bořek‑Dohalská L, Kizek R, Doktorová H, Eckschlager T and Stiborová M: Hypoxia-mediated histone acetylation and expression of N-myc transcription factor dictate aggressiveness of neuroblastoma cells. Oncol Rep 31: 1928-1934, 2014.
APA
Poljaková, J., Groh, T., Gudino, Ž.O., Hraběta, J., Bořek‑Dohalská, L., Kizek, R. ... Stiborová, M. (2014). Hypoxia-mediated histone acetylation and expression of N-myc transcription factor dictate aggressiveness of neuroblastoma cells. Oncology Reports, 31, 1928-1934. https://doi.org/10.3892/or.2014.2999
MLA
Poljaková, J., Groh, T., Gudino, Ž. O., Hraběta, J., Bořek‑Dohalská, L., Kizek, R., Doktorová, H., Eckschlager, T., Stiborová, M."Hypoxia-mediated histone acetylation and expression of N-myc transcription factor dictate aggressiveness of neuroblastoma cells". Oncology Reports 31.4 (2014): 1928-1934.
Chicago
Poljaková, J., Groh, T., Gudino, Ž. O., Hraběta, J., Bořek‑Dohalská, L., Kizek, R., Doktorová, H., Eckschlager, T., Stiborová, M."Hypoxia-mediated histone acetylation and expression of N-myc transcription factor dictate aggressiveness of neuroblastoma cells". Oncology Reports 31, no. 4 (2014): 1928-1934. https://doi.org/10.3892/or.2014.2999
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