RC-6 ribonuclease induces caspase activation, cellular senescence and neuron-like morphology in NT2 embryonal carcinoma cells

Yiang,Giou-Teng; Tsai,Hsiu-Feng; Chen,Jer-Rong; Chou,Pei-Lun; Wu,Tsai-Kun; Liu,Hsiao-Chun; Chang,Wei-Jung; Liu,Liang-Chih; Tseng,Hsu-Hung; Yu,Yung-Luen

doi:10.3892/or.2014.3023

RC-6 ribonuclease induces caspase activation, cellular senescence and neuron-like morphology in NT2 embryonal carcinoma cells

Authors:
- Giou-Teng Yiang
- Hsiu-Feng Tsai
- Jer-Rong Chen
- Pei-Lun Chou
- Tsai-Kun Wu
- Hsiao-Chun Liu
- Wei-Jung Chang
- Liang-Chih Liu
- Hsu-Hung Tseng
- Yung-Luen Yu
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Affiliations: Department of Emergency Medicine, Taipei Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, New Taipei 231, Taiwan, R.O.C., Department of Surgery, China Medical University Hospital, Taichung 404, Taiwan, R.O.C., Division of Allergy-Immunology-Rheumatology, Department of Internal Medicine, Saint Mary's Hospital Luodong, Yilan 265, Taiwan, R.O.C., Division of Renal Medicine, Tungs' Taichung Metroharbor Hospital, Taichung 435, Taiwan, R.O.C., Department of Nursing, Taipei Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, New Taipei 231, Taiwan, R.O.C., Graduate Institute of Cancer Biology and Center for Molecular Medicine, China Medical University, Taichung 404, Taiwan, R.O.C., Division of General Surgery, Taichung Hospital, Ministry of Health and Welfare, Taichung 403, Taiwan, R.O.C.
Published online on: February 11, 2014 https://doi.org/10.3892/or.2014.3023
Pages: 1738-1744

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Abstract

Frog ribonucleases have been demonstrated to have anticancer activities. However, whether RC-6 ribonuclease exerts anticancer activity on human embryonal carcinoma cells remains unclear. In the present study, RC-6 induced cytotoxicity in NT2 cells (a human embryonal carcinoma cell line) and our studies showed that RC-6 can exert anticancer effects and induce caspase-9 and -3 activities. Moreover, to date, there is no evidence that frog ribonuclease-induced cytotoxicity effects are related to cellular senescence. Therefore, our studies showed that RC-6 can increase p16 and p21 protein levels and induce cellular senescence in NT2 cells. Notably, similar to retinoic acid-differentiated NT2 cells, neuron-like morphology was found on some remaining live cells after RC-6 treatment. In conclusion, our study is the first to demonstrate that RC-6 can induce cytotoxic effects, caspase-9/-3 activities, cellular senescence and neuron-like morphology in NT2 cells.

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