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Print ISSN: 1021-335X Online ISSN: 1791-2431
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2014-April Volume 31 Issue 4

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

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Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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International Journal of Epigenetics

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Article

Downregulation of Rap1 promotes 5-fluorouracil-induced apoptosis in hepatocellular carcinoma cell line HepG2

  • Authors:
    • Yong Zha
    • Ping Gan
    • Qian Yao
    • Feng-Ming Ran
    • Jing Tan
  • View Affiliations / Copyright

    Affiliations: Department of Abdominal Surgery, Yunnan Cancer Hospital (The Third Affiliated Hospital, Kunming Medical University), Kunming, Yunnan 650118, P.R. China, Yunnan Cancer Research Institute, Yunnan Cancer Hospital (The Third Affiliated Hospital, Kunming Medical University), Kunming, Yunnan 650118, P.R. China, Department of Pathology, Yunnan Cancer Hospital (The Third Affiliated Hospital, Kunming Medical University), Kunming, Yunnan 650118, P.R. China
  • Pages: 1691-1698
    |
    Published online on: February 19, 2014
       https://doi.org/10.3892/or.2014.3033
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Abstract

Recent studies have revealed that repressor/activator protein (Rap1) not only protects telomeres from sister chromatid exchange, but also functions in genomewide transcriptional regulation. Knockdown of Rap1 sensitizes breast cancer cells to adriamycin-induced apoptosis. However, little is known about the role of Rap1 in the progression of hepatocellular carcinoma (HCC). The present study aimed to investigate the functions of Rap1 in HCC progression and to determine whether targeting the Rap1 signaling pathway may be of therapeutic value against HCC. We found knockdown of Rap1 by microRNA (miRNA) interference enhanced significantly apoptosis and 5-fluorouracil (5-FU) chemosensitivity in HepG2 cell line. Rap1 miRNA downregulated nuclear factor-κB p65 (NF-κB p65) expression, and upregulated inhibitor of NF-κB (IκB) expression. In vivo, Rap1 miRNA combined with 5-FU treatment led to a significant reduction of tumor growth as compared with 5-FU alone. The results indicate that Rap1 miRNA can effectively enhance sensitivity of HepG2 cell line to 5-FU chemotherapy in vitro and in vivo.
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Copy and paste a formatted citation
Spandidos Publications style
Zha Y, Gan P, Yao Q, Ran F and Tan J: Downregulation of Rap1 promotes 5-fluorouracil-induced apoptosis in hepatocellular carcinoma cell line HepG2. Oncol Rep 31: 1691-1698, 2014.
APA
Zha, Y., Gan, P., Yao, Q., Ran, F., & Tan, J. (2014). Downregulation of Rap1 promotes 5-fluorouracil-induced apoptosis in hepatocellular carcinoma cell line HepG2. Oncology Reports, 31, 1691-1698. https://doi.org/10.3892/or.2014.3033
MLA
Zha, Y., Gan, P., Yao, Q., Ran, F., Tan, J."Downregulation of Rap1 promotes 5-fluorouracil-induced apoptosis in hepatocellular carcinoma cell line HepG2". Oncology Reports 31.4 (2014): 1691-1698.
Chicago
Zha, Y., Gan, P., Yao, Q., Ran, F., Tan, J."Downregulation of Rap1 promotes 5-fluorouracil-induced apoptosis in hepatocellular carcinoma cell line HepG2". Oncology Reports 31, no. 4 (2014): 1691-1698. https://doi.org/10.3892/or.2014.3033
Copy and paste a formatted citation
x
Spandidos Publications style
Zha Y, Gan P, Yao Q, Ran F and Tan J: Downregulation of Rap1 promotes 5-fluorouracil-induced apoptosis in hepatocellular carcinoma cell line HepG2. Oncol Rep 31: 1691-1698, 2014.
APA
Zha, Y., Gan, P., Yao, Q., Ran, F., & Tan, J. (2014). Downregulation of Rap1 promotes 5-fluorouracil-induced apoptosis in hepatocellular carcinoma cell line HepG2. Oncology Reports, 31, 1691-1698. https://doi.org/10.3892/or.2014.3033
MLA
Zha, Y., Gan, P., Yao, Q., Ran, F., Tan, J."Downregulation of Rap1 promotes 5-fluorouracil-induced apoptosis in hepatocellular carcinoma cell line HepG2". Oncology Reports 31.4 (2014): 1691-1698.
Chicago
Zha, Y., Gan, P., Yao, Q., Ran, F., Tan, J."Downregulation of Rap1 promotes 5-fluorouracil-induced apoptosis in hepatocellular carcinoma cell line HepG2". Oncology Reports 31, no. 4 (2014): 1691-1698. https://doi.org/10.3892/or.2014.3033
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