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Article

miR-1236-3p represses the cell migration and invasion abilities by targeting ZEB1 in high-grade serous ovarian carcinoma

  • Authors:
    • Yu Wang
    • Shi Yan
    • Xiaolin Liu
    • Wenjing Zhang
    • Yingwei Li
    • Ruifen Dong
    • Qing Zhang
    • Qifeng Yang
    • Cunzhong Yuan
    • Keng Shen
    • Beihua Kong
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University School of Medicine, Jinan, Shandong 250012, P.R. China, Department of Breast Surgery, Qilu Hospital, Shandong University School of Medicine, Jinan, Shandong 250012, P.R. China, Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Peking Union Medical College, Beijing 100730, P.R. China
  • Pages: 1905-1910
    |
    Published online on: February 24, 2014
       https://doi.org/10.3892/or.2014.3046
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Abstract

Ovarian cancer, particularly high-grade serous ovarian carcinoma (HG-SOC), is still the main cause of death among gynecological malignancies. However, the molecular mechanisms related to its malignant biological behavior are still unclear. Recent studies indicate that microRNAs (miRNAs) play an important role in tumor metastasis. Here, we report that miR-1236-3p expression was downregulated in HG-SOC when compared to that in normal fallopian tube tissue. Manipulation of miR-1236-3p significantly influenced the morphology, migration and invasion of ovarian cancer cell lines (A2780 and SKOV3). With dual-luciferase reporter assay, we demonstrated that miR‑1236-3p binds to the 3'UTR of zinc-finger E-box binding homeobox 1 (ZEB1) mRNA, and functions as a negative regulator of ZEB1. Furthermore, we revealed that manipulation of miR-1236-3p modulates ZEB1 expression and influences expression of its downstream genes E-cadherin and N-cadherin at both the mRNA and protein levels. We also found an inverse relationship between miR‑1236-3p and ZEB1 expression in the HG-SOC tissue samples. Taken together, our results indicate that miR-1236-3p regulates ovarian cancer metastasis by directly targeting ZEB1, and it may play an important role in the diagnosis and treatment of ovarian cancer.
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1 

Siegel R, Naishadham D and Jemal A: Cancer statistics, 2013. CA Cancer J Clin. 63:11–30. 2013. View Article : Google Scholar

2 

Integrated genomic analyses of ovarian carcinoma. Nature. 474:609–615. 2011. View Article : Google Scholar

3 

Levanon K, Crum C and Drapkin R: New insights into the pathogenesis of serous ovarian cancer and its clinical impact. J Clin Oncol. 26:5284–5293. 2008. View Article : Google Scholar : PubMed/NCBI

4 

Li J, Fadare O, Xiang L, Kong B and Zheng W: Ovarian serous carcinoma: recent concepts on its origin and carcinogenesis. J Hematol Oncol. 5:82012. View Article : Google Scholar : PubMed/NCBI

5 

Liu Z, Liu J, Segura MF, et al: MiR-182 overexpression in tumourigenesis of high-grade serous ovarian carcinoma. J Pathol. 228:204–215. 2012. View Article : Google Scholar

6 

Kim J, Coffey DM, Creighton CJ, Yu Z, Hawkins SM and Matzuk MM: High-grade serous ovarian cancer arises from fallopian tube in a mouse model. Proc Natl Acad Sci USA. 109:3921–3926. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Liu Z, Gersbach E, Zhang X, et al: miR-106a represses the RB tumor suppressor p130 to regulate cellular proliferation and differentiation in high-grade serous ovarian carcinoma. Mol Cancer Res. 11:1314–1325. 2013. View Article : Google Scholar

8 

Thiery JP, Acloque H, Huang RY and Nieto MA: Epithelial-mesenchymal transitions in development and disease. Cell. 139:871–890. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Vergara D, Merlot B, Lucot JP, et al: Epithelial-mesenchymal transition in ovarian cancer. Cancer Lett. 291:59–66. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Berx G, Raspé E, Christofori G, Thiery JP and Sleeman JP: Pre-EMTing metastasis? Recapitulation of morphogenetic processes in cancer. Clin Exp Metastasis. 24:587–597. 2007. View Article : Google Scholar : PubMed/NCBI

11 

Yang G, Yuan J and Li K: EMT transcription factors: implication in osteosarcoma. Med Oncol. 30:6972013. View Article : Google Scholar : PubMed/NCBI

12 

Spaderna S, Schmalhofer O, Wahlbuhl M, et al: The transcriptional repressor ZEB1 promotes metastasis and loss of cell polarity in cancer. Cancer Res. 68:537–544. 2008. View Article : Google Scholar : PubMed/NCBI

13 

Bartel DP: MicroRNAs: target recognition and regulatory functions. Cell. 136:215–233. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Das E, Jana NR and Bhattacharyya NP: MicroRNA-124 targets CCNA2 and regulates cell cycle in STHdhQ111/HdhQ111 cells. Biochem Biophys Res Commun. 437:217–224. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Nakano H, Yamada Y, Miyazawa T and Yoshida T: Gain-of-function microRNA screens identify miR-193a regulating proliferation and apoptosis in epithelial ovarian cancer cells. Int J Oncol. 42:1875–1882. 2013.PubMed/NCBI

16 

Icli B, Wara AK, Moslehi J, et al: MicroRNA-26a regulates pathological and physiological angiogenesis by targeting BMP/SMAD1 signaling. Circ Res. 113:1231–1241. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Calin GA, Dumitru CD, Shimizu M, et al: Frequent deletions and down-regulation of micro- RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia. Proc Natl Acad Sci USA. 99:15524–15529. 2002.PubMed/NCBI

18 

Dahiya N and Morin PJ: MicroRNAs in ovarian carcinomas. Endocr Relat Cancer. 17:F77–F89. 2010. View Article : Google Scholar : PubMed/NCBI

19 

Bendoraite A, Knouf EC, Garg KS, et al: Regulation of miR-200 family microRNAs and ZEB transcription factors in ovarian cancer: evidence supporting a mesothelial-to-epithelial transition. Gynecol Oncol. 116:117–125. 2010. View Article : Google Scholar : PubMed/NCBI

20 

Chen J, Wang L, Matyunina LV, Hill CG and McDonald JF: Overexpression of miR-429 induces mesenchymal-to-epithelial transition (MET) in metastatic ovarian cancer cells. Gynecol Oncol. 121:200–205. 2011. View Article : Google Scholar : PubMed/NCBI

21 

Cao M, Seike M, Soeno C, et al: MiR-23a regulates TGF-β-induced epithelial-mesenchymal transition by targeting E-cadherin in lung cancer cells. Int J Oncol. 41:869–875. 2012.

22 

Ru P, Steele R, Newhall P, Phillips NJ, Toth K and Ray RB: miRNA-29b suppresses prostate cancer metastasis by regulating epithelial-mesenchymal transition signaling. Mol Cancer Ther. 11:1166–1173. 2012. View Article : Google Scholar : PubMed/NCBI

23 

Yokobori T, Suzuki S, Tanaka N, et al: MiR-150 is associated with poor prognosis in esophageal squamous cell carcinoma via targeting the EMT inducer ZEB1. Cancer Sci. 104:48–54. 2013. View Article : Google Scholar

24 

Buys SS, Partridge E, Black A, et al: Effect of screening on ovarian cancer mortality: the Prostate, Lung, Colorectal and Ovarian (PLCO) Cancer Screening Randomized Controlled Trial. JAMA. 305:2295–2303. 2011. View Article : Google Scholar : PubMed/NCBI

25 

Sempere LF, Christensen M, Silahtaroglu A, et al: Altered microRNA expression confined to specific epithelial cell subpopulations in breast cancer. Cancer Res. 67:11612–11620. 2007. View Article : Google Scholar : PubMed/NCBI

26 

Schaefer A, Jung M, Mollenkopf HJ, et al: Diagnostic and prognostic implications of microRNA profiling in prostate carcinoma. Int J Cancer. 126:1166–1176. 2010.PubMed/NCBI

27 

Sato K, Yoshimura A, Kaneko T, et al: A single nucleotide polymorphism in 3′-untranslated region contributes to the regulation of Toll-like receptor 4 translation. J Biol Chem. 287:25163–25172. 2012.

28 

Jones D, Li Y, He Y, Xu Z, Chen H and Min W: Mirtron microRNA-1236 inhibits VEGFR-3 signaling during inflammatory lymphangiogenesis. Arterioscler Thromb Vasc Biol. 32:633–642. 2012. View Article : Google Scholar : PubMed/NCBI

29 

Yokoyama Y, Charnock-Jones DS, Licence D, et al: Vascular endothelial growth factor-D is an independent prognostic factor in epithelial ovarian carcinoma. Br J Cancer. 88:237–244. 2003. View Article : Google Scholar : PubMed/NCBI

30 

Kim KH, Jo MS, Suh DS, et al: Expression and significance of the TLR4/MyD88 signaling pathway in ovarian epithelial cancers. World J Surg Oncol. 10:1932012. View Article : Google Scholar : PubMed/NCBI

31 

Zhu Y, Huang JM, Zhang GN, Zha X and Deng BF: Prognostic significance of MyD88 expression by human epithelial ovarian carcinoma cells. J Transl Med. 10:772012. View Article : Google Scholar : PubMed/NCBI

32 

Klasa-Mazurkiewicz D, Jarząb M, Milczek T, Lipińska B and Emerich J: Clinical significance of VEGFR-2 and VEGFR-3 expression in ovarian cancer patients. Pol J Pathol. 62:31–40. 2011.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang Y, Yan S, Liu X, Zhang W, Li Y, Dong R, Zhang Q, Yang Q, Yuan C, Shen K, Shen K, et al: miR-1236-3p represses the cell migration and invasion abilities by targeting ZEB1 in high-grade serous ovarian carcinoma. Oncol Rep 31: 1905-1910, 2014.
APA
Wang, Y., Yan, S., Liu, X., Zhang, W., Li, Y., Dong, R. ... Kong, B. (2014). miR-1236-3p represses the cell migration and invasion abilities by targeting ZEB1 in high-grade serous ovarian carcinoma. Oncology Reports, 31, 1905-1910. https://doi.org/10.3892/or.2014.3046
MLA
Wang, Y., Yan, S., Liu, X., Zhang, W., Li, Y., Dong, R., Zhang, Q., Yang, Q., Yuan, C., Shen, K., Kong, B."miR-1236-3p represses the cell migration and invasion abilities by targeting ZEB1 in high-grade serous ovarian carcinoma". Oncology Reports 31.4 (2014): 1905-1910.
Chicago
Wang, Y., Yan, S., Liu, X., Zhang, W., Li, Y., Dong, R., Zhang, Q., Yang, Q., Yuan, C., Shen, K., Kong, B."miR-1236-3p represses the cell migration and invasion abilities by targeting ZEB1 in high-grade serous ovarian carcinoma". Oncology Reports 31, no. 4 (2014): 1905-1910. https://doi.org/10.3892/or.2014.3046
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y, Yan S, Liu X, Zhang W, Li Y, Dong R, Zhang Q, Yang Q, Yuan C, Shen K, Shen K, et al: miR-1236-3p represses the cell migration and invasion abilities by targeting ZEB1 in high-grade serous ovarian carcinoma. Oncol Rep 31: 1905-1910, 2014.
APA
Wang, Y., Yan, S., Liu, X., Zhang, W., Li, Y., Dong, R. ... Kong, B. (2014). miR-1236-3p represses the cell migration and invasion abilities by targeting ZEB1 in high-grade serous ovarian carcinoma. Oncology Reports, 31, 1905-1910. https://doi.org/10.3892/or.2014.3046
MLA
Wang, Y., Yan, S., Liu, X., Zhang, W., Li, Y., Dong, R., Zhang, Q., Yang, Q., Yuan, C., Shen, K., Kong, B."miR-1236-3p represses the cell migration and invasion abilities by targeting ZEB1 in high-grade serous ovarian carcinoma". Oncology Reports 31.4 (2014): 1905-1910.
Chicago
Wang, Y., Yan, S., Liu, X., Zhang, W., Li, Y., Dong, R., Zhang, Q., Yang, Q., Yuan, C., Shen, K., Kong, B."miR-1236-3p represses the cell migration and invasion abilities by targeting ZEB1 in high-grade serous ovarian carcinoma". Oncology Reports 31, no. 4 (2014): 1905-1910. https://doi.org/10.3892/or.2014.3046
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