Shikonin blocks migration and invasion of human breast cancer cells through inhibition of matrix metalloproteinase-9 activation

Jang,Soon Young; Lee,Jae Koan; Jang,Eun Hyang; Jeong,Seo Young; Kim,Jong-Ho

doi:10.3892/or.2014.3159

Shikonin blocks migration and invasion of human breast cancer cells through inhibition of matrix metalloproteinase-9 activation

Authors:
- Soon Young Jang
- Jae Koan Lee
- Eun Hyang Jang
- Seo Young Jeong
- Jong-Ho Kim
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Affiliations: College of Pharmacy, Kyung Hee University, Seoul 130-701, Republic of Korea, Department of Life and Nanopharmaceutical Science, Kyung Hee University, Seoul 130-701, Republic of Korea
Published online on: April 25, 2014 https://doi.org/10.3892/or.2014.3159
Pages: 2827-2833

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Abstract

Shikonin, a natural naphthoquinone isolated from a traditional Chinese medicinal herb, has been reported to promote tumor cell death. However, there are few reports concerning its effect on metastasis-related cell invasion and migration behavior. In the present study, we investigated the effect of shikonin on human breast cancer invasion and migration. We found that shikonin inhibited phorbol 12-myristate 13-acetate (PMA)-induced cell migration and invasion in MCF-7 breast cancer cells, which was correlated with modulation of matrix metalloproteinase-9 (MMP-9) through suppression of both expression and proteolytic and promoter activity. We also found that shikonin inhibited both MMP-9 expression and promoter activity in MDA-MB‑231 cells with high metastatic potential. These results indicated that shikonin induces the suppression of migration and invasion through modulation of MMP-9 in human breast cancer cells. Therefore, shikonin may be a potential anticancer drug for human breast cancer therapy.

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