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Article

Dihydromyricetin induces cell cycle arrest and apoptosis in melanoma SK-MEL-28 cells

  • Authors:
    • Guofang Zeng
    • Jie Liu
    • Hege Chen
    • Bin Liu
    • Qingyu Zhang
    • Mingyi Li
    • Runzhi Zhu
  • View Affiliations / Copyright

    Affiliations: Zhanjiang Key Laboratory of Hepatobiliary Diseases, Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong 524001, P.R. China, Laboratory of Urology, Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong 524001, P.R. China
  • Pages: 2713-2719
    |
    Published online on: April 25, 2014
       https://doi.org/10.3892/or.2014.3160
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Abstract

Dihydromyricetin (DHM) exhibits multiple pharmacological activities; however, the role of DHM in anti-melanoma activities and the underlying molecular mechanisms are unclear. The aim of the present study was to evaluate the effects of DHM on cell proliferation, cell cycle distribution and apoptosis in the human melanoma SK-MEL-28 cell line, and to explore the related mechanisms. The effect of DHM on cell proliferation was investigated by MTT assay, and cell cycle distribution was determined by flow cytometry. TUNEL assay was used to evaluate DHM-mediated apoptosis, and western blotting was applied to examine expression levels of p53, p21, Cdc25A, Cdc2, P-Cdc2, Bax, IKK-α, NF-κB p65, p38 and P-p38 proteins. The results revealed that DHM suppressed cell proliferation of SK-MEL-28 cells in a concentration- and time-dependent manner, and caused cell cycle arrest at the G1/S phase. DHM increased the production of p53 and p21 proteins and downregulated the production of Cdc25A, Cdc2 and P-Cdc2 proteins, which induced cell cycle arrest. Additionally, DHM significantly induced the apoptosis of SK-MEL-28 cells, and enhanced the expression levels of Bax proteins and decreased the protein levels of IKK-α, NF-κB (p65) and P-p38. The results suggest that DHM may be a novel and effective candidate agent to inhibit the growth of melanoma.
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Copy and paste a formatted citation
Spandidos Publications style
Zeng G, Liu J, Chen H, Liu B, Zhang Q, Li M and Zhu R: Dihydromyricetin induces cell cycle arrest and apoptosis in melanoma SK-MEL-28 cells. Oncol Rep 31: 2713-2719, 2014.
APA
Zeng, G., Liu, J., Chen, H., Liu, B., Zhang, Q., Li, M., & Zhu, R. (2014). Dihydromyricetin induces cell cycle arrest and apoptosis in melanoma SK-MEL-28 cells. Oncology Reports, 31, 2713-2719. https://doi.org/10.3892/or.2014.3160
MLA
Zeng, G., Liu, J., Chen, H., Liu, B., Zhang, Q., Li, M., Zhu, R."Dihydromyricetin induces cell cycle arrest and apoptosis in melanoma SK-MEL-28 cells". Oncology Reports 31.6 (2014): 2713-2719.
Chicago
Zeng, G., Liu, J., Chen, H., Liu, B., Zhang, Q., Li, M., Zhu, R."Dihydromyricetin induces cell cycle arrest and apoptosis in melanoma SK-MEL-28 cells". Oncology Reports 31, no. 6 (2014): 2713-2719. https://doi.org/10.3892/or.2014.3160
Copy and paste a formatted citation
x
Spandidos Publications style
Zeng G, Liu J, Chen H, Liu B, Zhang Q, Li M and Zhu R: Dihydromyricetin induces cell cycle arrest and apoptosis in melanoma SK-MEL-28 cells. Oncol Rep 31: 2713-2719, 2014.
APA
Zeng, G., Liu, J., Chen, H., Liu, B., Zhang, Q., Li, M., & Zhu, R. (2014). Dihydromyricetin induces cell cycle arrest and apoptosis in melanoma SK-MEL-28 cells. Oncology Reports, 31, 2713-2719. https://doi.org/10.3892/or.2014.3160
MLA
Zeng, G., Liu, J., Chen, H., Liu, B., Zhang, Q., Li, M., Zhu, R."Dihydromyricetin induces cell cycle arrest and apoptosis in melanoma SK-MEL-28 cells". Oncology Reports 31.6 (2014): 2713-2719.
Chicago
Zeng, G., Liu, J., Chen, H., Liu, B., Zhang, Q., Li, M., Zhu, R."Dihydromyricetin induces cell cycle arrest and apoptosis in melanoma SK-MEL-28 cells". Oncology Reports 31, no. 6 (2014): 2713-2719. https://doi.org/10.3892/or.2014.3160
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