Open Access

Transforming growth factor-β1 suppresses bone morphogenetic protein-2-induced mesenchymal-epithelial transition in HSC-4 human oral squamous cell carcinoma cells via Smad1/5/9 pathway suppression

  • Authors:
    • Takahiro Chiba
    • Akira Ishisaki
    • Seiko Kyakumoto
    • Toshiyuki Shibata
    • Hiroyuki Yamada
    • Masaharu Kamo
  • View Affiliations

  • Published online on: December 28, 2016     https://doi.org/10.3892/or.2016.5338
  • Pages: 713-720
  • Copyright: © Chiba et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: HTML 0 views | PDF 0 views     Cited By (CrossRef): 0 citations

Abstract

Squamous cell carcinoma is the most common cancer in the oral cavity. We previously demonstrated that transforming growth factor-β1 (TGF-β1) promotes the epithelial-mesenchymal transition (EMT) of human oral squamous cell carcinoma (hOSCC) cells; however, it remains to be clarified whether the TGF-β superfamily member bone morphogenetic protein (BMP) affects this process in hOSCC cells. Here, we examined the independent and collective effects of TGF-β1 and BMP-2 on EMT and mesenchymal‑epithelial transition (MET) in a panel of four hOSCC cell lines. Notably, we found that HSC-4 cells were the most responsive to BMP-2 stimulation, which resulted in the upregulation of Smad1/5/9 target genes such as the MET inducers ID1 and cytokeratin 9 (CK9). Furthermore, BMP-2 downregulated the mesenchymal marker N-cadherin and the EMT inducer Snail, but upregulated epithelial CK9 expression, indicating that BMP-2 prefers to induce MET rather than EMT. Moreover, TGF-β1 dampened BMP-2-induced epithelial gene expression by inhibiting Smad1/5/9 expression and phosphorylation. Functional analysis revealed that TGF-β1 and BMP-2 significantly enhanced HSC-4 cell migration and proliferation, respectively. Collectively, these data suggest that TGF-β positively regulates hOSCC invasion in the primary tumor, whereas BMP-2 facilitates cancer cell colonization at secondary metastatic sites. Thus, the invasive and metastatic characteristics of hOSCC appear to be reciprocally regulated by BMP and TGF-β.

Introduction

Epithelial-mesenchymal transition (EMT) is characterized by the loss of epithelial markers with a reciprocal gain in mesenchymal phenotype and migratory potential (1). While EMT is essential for embryonic development and adult tissue maintenance (2,3), it is also necessary for desmoplasia and cancer cell migration (4). Conversely, mesenchymal-epithelial transition (MET) is a physiological and embryological phenomenon induced by cytokines (5,6). During EMT, transcription factors, such as Snail (7) and Slug (8) are upregulated and induce mesenchymal gene expression and suppress that of epithelial genes (9).

Transforming growth factor-β (TGF-β) is an important inducer of EMT (10,11). Bone morphogenetic proteins (BMPs) are members of the TGF-β superfamily, of which 20 have been discovered in humans to date (12). BMPs were first identified for their pro-osteogenic effects, but recent studies have revealed their additional significance as tissue morphogenetic factors (13), particularly for BMP-2, −4 and −7 (14). In particular, BMP-2 is a cytokine used to treat bone defects and is being investigated in regenerative studies (15,16).

BMP signaling is induced when a heterodimeric membrane kinase binds BMP and subsequently triggers Smad protein phosphorylation, similar to the mechanism of TGF-β pathway activation. However, downstream BMP-induced signals are mediated by Smad1/5/9, whereas TGF-β signaling is mediated by Smad2/3. These receptor-regulated Smad complexes (Smad1/5/9 and Smad2/3) bind the common mediator Smad4, and collectively translocate into the nucleus. The Smad complex then binds the DNA promoter region upstream of target genes to induce their expression (17). The effects of BMP on cancer cells are relatively uncharacterized when compared to those of TGF-β, but generally thought to promote EMT (18,19). However, BMP-2 was recently reported to suppress EMT in the presence of TGF-β (20). Thus, the overall effects of BMP on EMT likely depend on the cellular context.

Squamous cell carcinoma is the most common cancer of the oral cavity in humans (21,22). Although BMPs are thought to be involved in cancer metastasis, the mechanisms underlying BMP-induced EMT/MET have not yet been clarified at the molecular level (12). Nevertheless, a few studies have demonstrated the positive effects of BMP on cancer progression using human oral squamous cell carcinoma (hOSCC) cell lines and carcinoma tissue (2326). Moreover, while bone is a major target for hOSCC metastasis (26,27), the cellular and molecular mechanisms facilitating this process remain to be elucidated.

Previous studies by our group revealed that TGF-β1 responsiveness was correlated with EMT-related gene expression in six hOSCC cell lines. Notably, TGF-β1 enhanced the migration of HSC-4 hOSCC cells via the Slug/Wnt-5b/MMP-10 and integrin α3β1/FAK signaling axes (28,29). Since cells are subjected to multiple simultaneous signals from extracellular ligands and must then integrate and interpret them, this study investigated the independent and collective effects of TGF-β1 and BMP-2 on EMT and MET in HSC-4 cells. In addition, we evaluated how TGF-β1 affects the BMP-2-induced MET in HSC-4 cells at the molecular level.

Materials and methods

Materials

Cell lines were obtained from the Japanese Collection of Research Bioresources (Osaka, Japan). Recombinant human TGF-β1 and BMP-2 were purchased from Peprotech, Inc. (Rocky Hill, NJ, USA). The TGF-βR1 kinase inhibitor SB431542 was provided by Merck-Millipore (Frankfurt, Germany). The BMP type I receptor kinase inhibitor LDN-193189 was purchased from Selleck Chemical (Houston, TX, USA). The proteasome inhibitor MG132 was obtained from Merck Millipore. The protease inhibitor cocktail and phosphatase inhibitor cocktail 1 and 2 were purchased from Sigma-Aldrich (St. Louis, MO, USA). All of the other reagents were of analytical grade.

Cell culture

All of the cell lines were grown at 37°C in 5% CO2. Human HSC-2 and HSC-4 squamous cell carcinoma cells were cultured in MEM (Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS) (Gibco, Rockville, MD, USA). SAS cells were cultured in RPMI-1640 medium (Gibco) supplemented with 10% FBS. HSC-3 cells were cultured in DMEM (Sigma-Aldrich) containing 10% FBS. The culture medium was replaced with serum-free medium 24 h prior to cytokine-stimulated experiments. In the majority of experiments, 2.0×105 hOSCC cells were cultured in 500 µl serum-free media containing 10 ng/ml TGF-β1 or 20 ng/ml BMP-2 for the indicated time-periods in 24-well tissue culture plates.

Western blot analysis

For Smad phosphorylation analysis, 3.0×106 cells were lysed in RIPA buffer (Sigma-Aldrich) containing a protease and phosphatase inhibitor cocktail (Sigma-Aldrich) and the protein content was assessed with a BCA reagent (Thermo Fisher Scientific, Waltham, MA, USA). To examine marker protein expression, 1.0×106 cells were cultured in a 6-well plate in serum-free MEM with or without 10 ng/ml TGF-β1 for the indicated time-points. Harvested cells were homogenized in SDS sample buffer containing a protease and phosphatase inhibitor cocktail (Sigma-Aldrich). Proteins were separated by 12.5% SDS-PAGE (ATTO Co., Tokyo, Japan) and subsequently transferred onto PVDF membranes (Merck Millipore). The membranes were probed with the primary antibodies, including rabbit anti-Smad1 (#6944) and rabbit anti-Smad5 (#9517; both from Cell Signaling Technology, Beverly, MA, USA), rabbit anti-Smad9 [Smad8 (R-64); Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA] and rabbit anti-phospho-Smad1 (Ser463/465)/Smad5 (Ser463/465)/Smad9 (Ser465/467) (#13820; Cell Signaling Technology). Mouse anti-β-actin (clone C4; Santa Cruz Biotechnology, Inc.) was used as a loading control in the Smad phosphorylation experiments. The blots were then incubated with alkaline phosphatase-conjugated secondary antibody and signals were visualized using an alkaline phosphatase substrate kit (BCIP/NBT substrate kit; Vector Laboratories, Inc., Burlingame, CA, USA).

Reverse transcriptase quantitative-PCR (RT-qPCR)

For total RNA preparation, 2.0×105 cells were cultured in 24-well tissue culture plates. Total RNA was isolated using the Isogen reagent (Nippon Gene, Co., Ltd., Toyama, Japan) according to the manufacturer's instructions. RNA was reverse transcribed into first-strand cDNA with an RT-PCR system kit (Takara Bio, Inc., Shiga, Japan). qPCR was performed on a Thermal Cycler Dice Real-Time System (Takara Bio, Inc.) using SYBR® Premix Ex Taq™ II (Takara Bio, Inc.) with human gene-specific primers (Table I). Target gene expression was normalized to an internal β-actin reference and expressed in terms of fold-change relative to the control sample (30).

Table I.

Primer sequences for the qPCR analysis.

Table I.

Primer sequences for the qPCR analysis.

GenesPrimer sequences
BMP-2F 5′-AAGATTCCTAAGGCATGCTGTGTC-3′
R 5′-TCGTCAAGGTACAGCATCGAGA-3′
Cytokeratin 9F 5′-TCAGCTGACTGGGCAGAACA-3′
R 5′-ACCTCATGCTCGATCTGGGTTA-3′
Cytokeratin 18F 5′-AGGAGTATGAGGCCCTGCTGAA-3′
R 5′-TTGCATGGAGTTGCTGCTGTC-3′
E-cadherinF 5′-TACACTGCCCAGGAGCCAGA-3′
R 5′-TGGCACCAGTGTCCGGATTA-3′
ID1F 5′-CGGAATCTGAGGGAGAACAAG-3′
R 5′-CTGAGAAGCACCAAACGTGA-3′
N-cadherinF 5′-CGAATGGATGAAAGACCCATCC-3′
R 5′-TCGTCAAGGTACAGCATCGAGA-3′
NEDD4F 5′-GATTTGTAAACCGAATCCAGAAGCA-3′
R 5′-CCAGTCATTCACATCAACATCTCC-3′
NEDD4LF 5′-CCAATGGGTCAGAAATAATGGTCA-3′
R 5′-AAGGCGTTCATCTGCTTCTGG-3′
Smad1F 5′-ACAGTCTGTGAACCATGGATTTGA-3′
R 5′-TGAGGTGAACCCATTTGAGTAAGAA-3′
Samd5F 5′-GCTTTCATCCCACCACTGTCTGTA-3′
R 5′-CCTGCCGGTGATATTCTGCTC-3′
Smad6F 5′-GAGCTGAGCCGAGAGAAAGA-3′
R 5′-AGATGCACTTGGAGCGAGTT-3′
Smad7F 5′-TGCAACCCCCATCACCTTAG-3′
R 5′-TCGTCAAGGTACAGCATCGAGA-3′
Smad9F 5′-TGGCCCAGTCAGTTCACCAC-3′
R 5′-CATGAAGATGAATCTCAATCCAGCA-3′
Smurf1F 5′-CCGCATCGAAGTGTCCAGAG-3′
R 5′-CCCACGGAATTTCACCATCAG-3′
Smurf2F 5′-TGCACTAACAACCTGCCGAAAG-3′
R 5′-CTTGTCATTCCACAGCAAATCCAC-3′
SnailF 5′-GACCACTATGCCGCGCTCTT-3′
R 5′-TCGCTGTAGTTAGGCTTCCGATT-3′
β-actinF 5′-GGAGATTACTGCCCTGGCTCCTA-3′
R 5′-GACTCATCGTACTCCTGCTTGCTG-3′

[i] F, forward; R, reverse.

Proliferation assay

HSC-4 cell proliferation was evaluated by alamarBlue® assay (AbD Serotec, Oxon, UK) according to the manufacturer's instructions. Briefly, 1.0×104 cells/well were subcultured in 96-well plates in MEM supplemented with 10% FBS for 24 h. The culture medium was then replaced with MEM supplemented without FBS with or without TGF-β1 (10 ng/ml) or BMP-2 (20 ng/ml) for 2 days. The cells were washed once with PBS and then incubated with 100 µl alamarBlue® (10% alamarBlue® in Ham's F-12) at 37°C in 5% CO2 for 1.5 h. The absorbances at 570 and 600 nm were used to measure the reduced and oxidized forms of reagent, respectively, using a microplate reader. Assays were performed independently at least 3 times (n=6).

Cell migration assays

Boyden chamber migration assays were performed using cells transfected with Slug siRNA as aforementioned. Then, cells were treated with 10 ng/ml TGF-β1 or 20 ng/ml BMP-2 under serum-free conditions for 72 h and subsequently plated at a density of 1.0×105 cells in the upper chamber of a Boyden apparatus in serum-free media, and were allowed to migrate into the lower chamber containing medium supplemented with 10% FBS for 24 h at 37°C. The chamber filter was fixed in 4% paraformaldehyde and stained with DAPI (1:500; Sigma-Aldrich) for 10 min. Migrating cells were counted in 9 fields on the membrane. Values indicate the mean number of migrating cells compared to the controls.

Statistical analysis

All experiments were performed at least 3 times independently. Results are expressed as the means ± standard deviation (SD). Data were analyzed using two-tailed, independent Student's t-tests. P<0.05 was considered statistically significant.

Results

BMP-2 responsiveness in the hOSCC cell lines

We first studied the cellular response to BMP-2 in four hOSCC cell lines: HSC-2, HSC-3, HSC-4, and SAS. Smad6, Smad7 and ID1 are targets of BMP signaling (31). Expression analysis revealed that BMP-2 induced a significant upregulation of all three target genes in the HSC-4 cells (Fig. 1A), but no marked changes were observed in the other cell lines (Fig. 1B-D). Thus, we used HSC-4 cells to investigate the effects of BMP-2 on MET in the following experiments. In addition, HSC-4 cells are also responsive to TGF-β1 stimulation based on our previous examinations (28).

BMP-2-induced response in HSC-4 cells

To confirm BMP-2 signaling in HSC-4 cells, the time course of Smad6 and ID1 gene expression was investigated in the presence or absence of LDN-193189, a selective inhibitor of activin receptor-like kinase (ALK)-2/3 (Fig. 2A and B). The results showed that both genes were upregulated 1 h after BMP-2 treatment, peaking at 3 h post-stimulation, which was significantly inhibited by LDN-193189. Moreover, Smad1/5/9 phosphorylation was increased in the BMP-2-treated HSC-4 cells, but inhibited in the presence of LDN-193189 (Fig. 2C). Thus, these results indicated that BMP-2 elicits Smad1/5/9 activation and target gene expression in HSC-4 cells.

Effects of BMP-2 or TGF-β1 on epithelial and mesenchymal status in HSC-4 cells

Next, we examined how BMP-2 alters the epithelial and mesenchymal characteristics of HSC-4 cells by examining marker expression with RT-qPCR (Fig. 3). Notably, the epithelial marker cytokeratin 9 (CK9) was clearly upregulated following BMP-2-stimulation (Fig. 3A, right), whereas that of N-cadherin was significantly suppressed (Fig. 3C, left). Conversely, TGF-β1 stimulation resulted in the significant suppression of epithelial cytokeratin 18 (CK18) marker (Fig. 3B, right), as well as the induction of the mesenchymal markers N-cadherin and vimentin (Fig. 3D). However, the expression of epithelial marker E-cadherin was not affected by either BMP-2 or TGF-β1 stimulation (Fig. 3A and B, left), and mesenchymal vimentin was not significantly affected by BMP-2 (Fig. 3C, right). Moreover, the morphology of BMP-2-treated HSC-4 cells was not different from that of control cells (data not shown). Collectively, these data indicated that TGF-β1 suppresses epithelial marker expression and promotes that of mesenchymal markers, resulting in EMT. In contrast, BMP-2 has the opposite effect promoting MET.

TGF-β1 abrogates BMP-2-mediated effects on MET/EMT gene expression in a dose-dependent manner

To examine the function of TGF-β1 on BMP-2-induced MET, HSC-4 cells were treated with increasing doses of TGF-β1 with or without BMP-2 stimulation. BMP-2-induced epithelial CK9 (Fig. 4A, left) and ID1 upregulation (Fig. 2B), whereas the N-cadherin and Snail downregulation (Fig. 4A, right) was significantly suppressed by LDN-193189. Moreover, BMP-2-induced CK9 and ID1 upregulation was significantly suppressed by TGF-β1 in a dose-dependent manner (Fig. 4B and C). Similarly, BMP-2-induced N-cadherin and Snail downregulation were significantly suppressed by TGF-β1 stimulation in a dose-dependent manner (Fig. 4D and E).

Effect of TGF-β1 on Smad1/5/9 expression and phosphorylation

Western blot analysis showed that TGF-β1 hindered Smad1/5/9 phosphorylation in HSC-4 cells in the presence or absence of BMP-2 (Fig. 5A). In addition, while TGF-β1 suppressed Smad1 expression independently of BMP-2, it had no marked effect on Smad5 expression. Smad9 expression was undetectable regardless of exogenous stimulation. With respect to RNA expression, both Smad1 and 9 were significantly decreased by TGF-β1 stimulation, whereas that of Smad5 was unaffected (Fig. 5B). TGF-β1 stimulation alters the levels of phosphorylated (p)Smad1/5 and pSmad2 by regulating the E3 ligase activities of Smurf1 (32,33), NEDD4 (34) Smurf2 (35) and NEDD4L (36), respectively. Notably, Smurf1 and NEDD4 were significantly upregulated by TGF-β1 stimulation, whereas Smurf2 and NEDD4L were mostly unchanged (Fig. 5C). However, the TGF-β1-mediated degradation of BMP-2-induced pSmad1/5/9 remained intact after treatment with MG132 proteasome inhibitor. This suggested that BMP signal attenuation by TGF-β1 occurs in a proteasome-independent manner (Fig. 5D), possibly through the Smad1/9 downregulation (Fig. 5B). Thus, the effect of TGF-β1 on BMP-2 signaling likely results from the suppression of Smad1/9 expression rather than phosphorylation.

Effect of BMP-2 and TGF-β1 on HSC-4 cell migration and proliferation

The effect of BMP-2 on cell migration and proliferation was investigated to assess its potential effect on hOSCC progression (Fig. 6). This analysis revealed that TGF-β1 significantly enhanced the migratory capacity of HSC-4 cells 72 h after TGF-β1 stimulation, whereas BMP-2 did not (Fig. 6A). Alternatively, BMP-2 facilitated cell proliferation 48 h after stimulation, but was significantly suppressed in the presence of TGF-β1 (Fig. 6B).

Discussion

In order to detect BMP-2-responsive hOSCC, Smad6 and ID1 expression (Fig. 1) and Smad1/5/9 phosphorylation (Fig. 2) were examined in four hOSCC cell lines after BMP-2 stimulation. HSC-4 cells were the most responsive to both BMP-2 and TGF-β1 and thus selected for further analysis. Results from the present study demonstrate that BMP-2 promoted the expression of epithelial CK9, but suppressed that of the mesenchymal markers N-cadherin and vimentin (Fig. 3C), suggesting that it promotes MET. Moreover, these BMP-2-inducible effects were significantly inhibited by TGF-β1 stimulation in HSC-4 cells in a dose-dependent manner (Fig. 4B-E). Collectively, these results strongly suggest that TGF-β1 inhibits BMP-2-induced MET in hOSCC.

Further analysis revealed that TGF-β1 suppressed BMP-2-induced Smad1/5/9 phosphorylation (Fig. 5A) and Smad1/9 expression (Fig. 5B) and promoted the expression of E3 protein ligases that target BMP-2 pathway effectors in HSC-4 cells (Fig. 5C). As such, TGF-β1 is likely a key regulator of Smad1/5/9 pathway suppression downstream of BMP-2 in hOSCC cells. ID (inhibitor of DNA binding) proteins are a family of four transcriptional regulators, including ID1 (31). ID1 expression is reportedly enhanced by BMP-2-mediated Smad1/5 pathway activity (Figs. 1A, 2B and 2C) (37,38). ID proteins have significant implications in cancer progression as Stankic et al reported that ID1 induced MET during metastatic breast cancer cell colonization (39). Moreover, Del Pozo Martin et al (40) reported that metastatic colonization is induced by the interaction between mesenchymal cancer cells and stromal fibroblasts, which secrete factors to induce MET via BMP/Smad1/5 signaling. Our study showed that ID1 protein expression was increased when cultured in activated fibroblast-conditioned media, but was blocked by LDN-193189 treatment. Based on these data, TGF-β1 may suppress MET by disrupting BMP-2-mediated Smad1/5/9 signaling, resulting in ID1 downregulation in HSC-4 cells. On the other hand, Snail is upregulated during EMT and generates a positive feedback loop (10). Notably, Snail expression was significantly suppressed by BMP-2 in HSC-4 cells (Fig. 4A, right and 4E); however, whether BMP-2-induced Smad1/5/9 signaling plays an important role in Snail suppression in HSC-4 cells remains unclear.

Cancer metastasis is the result of cancer cell MET, as well as their proliferative burst after homing to these metastatic sites (12,40). As shown in Fig. 6B, BMP-2 significantly induced HSC-4 cell proliferation, which was not observed following TGF-β1 treatment. If hOSCC cells are susceptible to BMP-2 stimulation at metastatic sites, they likely retain a high proliferative capacity to promote secondary tumor formation. Collectively, this evidence supports that BMP-2 positively regulates metastatic colonization in hOSCC. On the other hand, TGF-β1 induces EMT (28) and increases cell migration (Fig. 6A) and invasion (29) in primary hOSCC tumors. In addition, TGF-β1 may inhibit tumor progression by attenuating BMP-2-induced MET at metastatic sites.

Yang et al (20) reported that BMP-2 suppresses EMT in TGF-β1-induced renal interstitial fibrosis. Interestingly, BMP-2 attenuated TGF-β1-induced EMT of NRK-49F kidney fibroblasts downregulating Snail expression. Alternatively, we found that the BMP-2-induced Snail downregulation was significantly inhibited by TGF-β1 stimulation in a dose-dependent manner (Fig 4E), suggesting that TGF-β1 suppresses the BMP-2-induced MET by disrupting the induction of Snail in hOSCC cells. Recently, it was reported that BMP-4 may inhibit TGF-β1-induced EMT in primary retinal pigment epithelium cells through the Smad2/3 pathway (41). Therefore, it will be necessary to determine whether TGF-β1-induced EMT is inhibited by BMP stimulation in hOSCC cells in the future.

Our findings partly clarify the molecular mechanisms underlying EMT and MET in hOSCC and may facilitate the discovery of molecular drug targets to attenuate hOSCC progression.

Acknowledgements

We would like to thank Editage (www.editage.jp) for English language editing. This study was supported in part by a Grant-in-aid for Scientific Research (no. 90118274 to S.K., no. 26293426 to T.S. and no. 2667052 and 16H05534 to A.I.) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan; Grant-in-aid for the Strategic Medical Science Research Center from the Ministry of Education, Culture, Sports, Science and Technology of Japan, 2010–2014.

Glossary

Abbreviations

Abbreviations:

BMP

bone morphogenetic protein

CK9

cytokeratin 9

CK18

cytokeratin 18

EMT

epithelial-mesenchymal transition

hOSCC

human oral squamous cell carcinoma

MET

mesenchymal-epithelial transition

TGF-β

transforming growth factor-β

References

1 

Smith A, Teknos TN and Pan Q: Epithelial to mesenchymal transition in head and neck squamous cell carcinoma. Oral Oncol. 49:287–292. 2013. View Article : Google Scholar : PubMed/NCBI

2 

Thiery JP, Acloque H, Huang RY and Nieto MA: Epithelial- mesenchymal transitions in development and disease. Cell. 139:871–890. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Weber CE, Li NY, Wai PY and Kuo PC: Epithelial-mesenchymal transition, TGF-β, and osteopontin in wound healing and tissue remodeling after injury. J Burn Care Res. 33:311–318. 2012. View Article : Google Scholar : PubMed/NCBI

4 

He J, Xu Y, Koya D and Kanasaki K: Role of the endothelial-to-mesenchymal transition in renal fibrosis of chronic kidney disease. Clin Exp Nephrol. 17:488–497. 2013. View Article : Google Scholar : PubMed/NCBI

5 

Fabregat I, Malfettone A and Soukupova J: New insights into the crossroads between EMT and stemness in the context of cancer. J Clin Med. 5:372016. View Article : Google Scholar

6 

Moustakas A and Heldin P: TGFβ and matrix-regulated epithelial to mesenchymal transition. Biochim Biophys Acta. 1840:2621–2634. 2014. View Article : Google Scholar : PubMed/NCBI

7 

Batlle E, Sancho E, Francí C, Domínguez D, Monfar M, Baulida J and De Herreros A García: The transcription factor snail is a repressor of E-cadherin gene expression in epithelial tumour cells. Nat Cell Biol. 2:84–89. 2000. View Article : Google Scholar : PubMed/NCBI

8 

Medici D, Hay ED and Olsen BR: Snail and Slug promote epithelial-mesenchymal transition through β-catenin-T-cell factor-4-dependent expression of transforming growth factor-β3. Mol Biol Cell. 19:4875–4887. 2008. View Article : Google Scholar : PubMed/NCBI

9 

Zeisberg M and Neilson EG: Biomarkers for epithelial-mesenchymal transitions. J Clin Invest. 119:1429–1437. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Peinado H, Olmeda D and Cano A: Snail, Zeb and bHLH factors in tumour progression: An alliance against the epithelial phenotype? Nat Rev Cancer. 7:415–428. 2007. View Article : Google Scholar : PubMed/NCBI

11 

Lamouille S, Xu J and Derynck R: Molecular mechanisms of epithelial-mesenchymal transition. Nat Rev Mol Cell Biol. 15:178–196. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Wakefield LM and Hill CS: Beyond TGFβ: Roles of other TGFβ superfamily members in cancer. Nat Rev Cancer. 13:328–341. 2013. View Article : Google Scholar : PubMed/NCBI

13 

Simic P and Vukicevic S: Bone morphogenetic proteins in development and homeostasis of kidney. Cytokine Growth Factor Rev. 16:299–308. 2005. View Article : Google Scholar : PubMed/NCBI

14 

Bragdon B, Moseychuk O, Saldanha S, King D, Julian J and Nohe A: Bone morphogenetic proteins: A critical review. Cell Signal. 23:609–620. 2011. View Article : Google Scholar : PubMed/NCBI

15 

Wu M, Chen G and Li YP: TGF-β and BMP signaling in osteoblast, skeletal development and bone formation, homeostasis and disease. Bone Res. 4:160092016. View Article : Google Scholar : PubMed/NCBI

16 

Kopf J, Paarmann P, Hiepen C, Horbelt D and Knaus P: BMP growth factor signaling in a biomechanical context. Biofactors. 40:171–187. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Chen G, Deng C and Li YP: TGF-β and BMP signaling in osteoblast differentiation and bone formation. Int J Biol Sci. 8:272–288. 2012. View Article : Google Scholar : PubMed/NCBI

18 

McCormack N and O'Dea S: Regulation of epithelial to mesenchymal transition by bone morphogenetic proteins. Cell Signal. 25:2856–2862. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Kang MH, Kim JS, Seo JE, Oh SC and Yoo YA: BMP2 accelerates the motility and invasiveness of gastric cancer cells via activation of the phosphatidylinositol 3-kinase (PI3K)/Akt pathway. Exp Cell Res. 316:24–37. 2010. View Article : Google Scholar : PubMed/NCBI

20 

Yang YL, Ju HZ, Liu SF, Lee TC, Shih YW, Chuang LY, Guh JY, Yang YY, Liao TN, Hung TJ, et al: BMP-2 suppresses renal interstitial fibrosis by regulating epithelial-mesenchymal transition. J Cell Biochem. 112:2558–2565. 2011. View Article : Google Scholar : PubMed/NCBI

21 

Graves CA, Abboodi FF, Tomar S, Wells J and Pirisi L: The translational significance of epithelial-mesenchymal transition in head and neck cancer. Clin Transl Med. 3:602014. View Article : Google Scholar : PubMed/NCBI

22 

Lambert R, Sauvaget C, de Camargo Cancela M and Sankaranarayanan R: Epidemiology of cancer from the oral cavity and oropharynx. Eur J Gastroenterol Hepatol. 23:633–641. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Kokorina NA, Lewis JS Jr, Zakharkin SO, Krebsbach PH and Nussenbaum B: rhBMP-2 has adverse effects on human oral carcinoma cell lines in vivo. Laryngoscope. 122:95–102. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Kokorina NA, Zakharkin SO, Krebsbach PH and Nussenbaum B: Treatment effects of rhBMP-2 on invasiveness of oral carcinoma cell lines. Laryngoscope. 121:1876–1880. 2011.PubMed/NCBI

25 

Sand JP, Kokorina NA, Zakharkin SO, Lewis JS Jr and Nussenbaum B: BMP-2 expression correlates with local failure in head and neck squamous cell carcinoma. Otolaryngol Head Neck Surg. 150:245–250. 2014. View Article : Google Scholar : PubMed/NCBI

26 

Kejner AE, Burch MB, Sweeny L and Rosenthal EL: Bone morphogenetic protein 6 expression in oral cavity squamous cell cancer is associated with bone invasion. Laryngoscope. 123:3061–3065. 2013. View Article : Google Scholar : PubMed/NCBI

27 

Quan J, Johnson NW, Zhou G, Parsons PG, Boyle GM and Gao J: Potential molecular targets for inhibiting bone invasion by oral squamous cell carcinoma: A review of mechanisms. Cancer Metastasis Rev. 31:209–219. 2012. View Article : Google Scholar : PubMed/NCBI

28 

Saito D, Kyakumoto S, Chosa N, Ibi M, Takahashi N, Okubo N, Sawada S, Ishisaki A and Kamo M: Transforming growth factor-β1 induces epithelial-mesenchymal transition and integrin α3β1-mediated cell migration of HSC-4 human squamous cell carcinoma cells through Slug. J Biochem. 153:303–315. 2013. View Article : Google Scholar : PubMed/NCBI

29 

Hino M, Kamo M, Saito D, Kyakumoto S, Shibata T, Mizuki H and Ishisaki A: Transforming growth factor-β1 induces invasion ability of HSC-4 human oral squamous cell carcinoma cells through the Slug/Wnt-5b/MMP-10 signalling axis. J Biochem. 159:631–640. 2016. View Article : Google Scholar : PubMed/NCBI

30 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

31 

Lasorella A, Benezra R and Iavarone A: The ID proteins: Master regulators of cancer stem cells and tumour aggressiveness. Nat Rev Cancer. 14:77–91. 2014. View Article : Google Scholar : PubMed/NCBI

32 

Shi W, Chen H, Sun J, Chen C, Zhao J, Wang YL, Anderson KD and Warburton D: Overexpression of Smurf1 negatively regulates mouse embryonic lung branching morphogenesis by specifically reducing Smad1 and Smad5 proteins. Am J Physiol Lung Cell Mol Physiol. 286:L293–L300. 2004. View Article : Google Scholar : PubMed/NCBI

33 

Murakami G, Watabe T, Takaoka K, Miyazono K and Imamura T: Cooperative inhibition of bone morphogenetic protein signaling by Smurf1 and inhibitory Smads. Mol Biol Cell. 14:2809–2817. 2003. View Article : Google Scholar : PubMed/NCBI

34 

Kim BG, Lee JH, Yasuda J, Ryoo HM and Cho JY: Phospho-Smad1 modulation by nedd4 e3 ligase in BMP/TGF-β signaling. J Bone Miner Res. 26:1411–1424. 2011. View Article : Google Scholar : PubMed/NCBI

35 

Lin X, Liang M and Feng XH: Smurf2 is a ubiquitin E3 ligase mediating proteasome-dependent degradation of Smad2 in transforming growth factor-β signaling. J Biol Chem. 275:36818–36822. 2000. View Article : Google Scholar : PubMed/NCBI

36 

Gao S, Alarcón C, Sapkota G, Rahman S, Chen PY, Goerner N, Macias MJ, Erdjument-Bromage H, Tempst P and Massagué J: Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-β signaling. Mol Cell. 36:457–468. 2009. View Article : Google Scholar : PubMed/NCBI

37 

Ogata T, Wozney JM, Benezra R and Noda M: Bone morphogenetic protein 2 transiently enhances expression of a gene, Id (inhibitor of differentiation), encoding a helix-loop-helix molecule in osteoblast-like cells. Proc Natl Acad Sci USA. 90:9219–9222. 1993. View Article : Google Scholar : PubMed/NCBI

38 

Langenfeld EM, Kong Y and Langenfeld J: Bone morphogenetic protein 2 stimulation of tumor growth involves the activation of Smad-1/5. Oncogene. 25:685–692. 2006. View Article : Google Scholar : PubMed/NCBI

39 

Stankic M, Pavlovic S, Chin Y, Brogi E, Padua D, Norton L, Massagué J and Benezra R: TGF-β-Id1 signaling opposes Twist1 and promotes metastatic colonization via a mesenchymal-to-epithelial transition. Cell Reports. 5:1228–1242. 2013. View Article : Google Scholar : PubMed/NCBI

40 

Del Pozo Martin Y, Park D, Ramachandran A, Ombrato L, Calvo F, Chakravarty P, Spencer-Dene B, Derzsi S, Hill CS, Sahai E, et al: Mesenchymal cancer cell-stroma crosstalk promotes niche activation, epithelial reversion, and metastatic colonization. Cell Reports. 13:2456–2469. 2015. View Article : Google Scholar : PubMed/NCBI

41 

Yao H, Li H, Yang S, Li M, Zhao C, Zhang J, Xu G and Wang F: Inhibitory effect of bone morphogenetic protein 4 in retinal pigment epithelial-mesenchymal transition. Sci Rep. 6:321822016. View Article : Google Scholar : PubMed/NCBI

Related Articles

Journal Cover

February 2017
Volume 37 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Chiba, T., Ishisaki, A., Kyakumoto, S., Shibata, T., Yamada, H., & Kamo, M. (2017). Transforming growth factor-β1 suppresses bone morphogenetic protein-2-induced mesenchymal-epithelial transition in HSC-4 human oral squamous cell carcinoma cells via Smad1/5/9 pathway suppression. Oncology Reports, 37, 713-720. https://doi.org/10.3892/or.2016.5338
MLA
Chiba, T., Ishisaki, A., Kyakumoto, S., Shibata, T., Yamada, H., Kamo, M."Transforming growth factor-β1 suppresses bone morphogenetic protein-2-induced mesenchymal-epithelial transition in HSC-4 human oral squamous cell carcinoma cells via Smad1/5/9 pathway suppression". Oncology Reports 37.2 (2017): 713-720.
Chicago
Chiba, T., Ishisaki, A., Kyakumoto, S., Shibata, T., Yamada, H., Kamo, M."Transforming growth factor-β1 suppresses bone morphogenetic protein-2-induced mesenchymal-epithelial transition in HSC-4 human oral squamous cell carcinoma cells via Smad1/5/9 pathway suppression". Oncology Reports 37, no. 2 (2017): 713-720. https://doi.org/10.3892/or.2016.5338