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Diagnostic and prognostic values of integrin α subfamily mRNA expression in colon adenocarcinoma

  • Authors:
    • Yi‑Zhen Gong
    • Guo‑Tian Ruan
    • Xi‑Wen Liao
    • Xiang‑Kun Wang
    • Cun Liao
    • Shuai Wang
    • Feng Gao
  • View Affiliations / Copyright

    Affiliations: Department of Colorectal and Anal Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China, Department of Hepatobiliary Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China
    Copyright: © Gong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 923-936
    |
    Published online on: June 28, 2019
       https://doi.org/10.3892/or.2019.7216
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Abstract

The integrin α (ITGA) subfamily genes play a fundamental role in various cancers. However, the potential mechanism and application values of ITGA genes in colon adenocarcinoma (COAD) remain elusive. The present study investigated the significance of the expression of ITGA genes in COAD from the perspective of diagnosis and prognosis. A COAD RNA‑sequencing dataset was obtained from The Cancer Genome Atlas. The present study investigated the biological function of the ITGA subfamily genes through bioinformatics analysis. Reverse transcription‑quantitative polymerase chain reaction was applied to investigate the distribution of integrin α8 (ITGA8) expression in COAD tumors and adjacent normal tissues. Bioinformatics analysis indicated that ITGA genes were noticeably enriched in cell adhesion and the integrin‑mediated signaling pathway, and co‑expressed with each other. It was also revealed through observation that the majority of gene expression was significantly low in tumor tissues (P<0.05), and diagnostic receiver operating characteristic curves revealed that most of the genes could serve as significant diagnostic markers in COAD (P<0.05), especially ITGA8 which had a high diagnostic value with an area under curve (AUC) of 0.989 [95% confidence interval (CI) 0.980‑0.997] in COAD (P<0.0001). In addition, ITGA8 expression was verified in clinical samples and it was revealed that it was higher in adjacent normal tissues (P=0.041) compared to COAD tissues, and the AUC was 0.704 (95% CI, 0.577‑0.831; P<0.0085). Multivariate survival analysis indicated that integrin α (ITGA5) may be an independent prognostic indicator for COAD overall survival. Gene set enrichment analysis indicated that ITGA5 may participate in multiple biological processes and pathways. The present study revealed that ITGA genes were associated with the diagnosis and prognosis of COAD. The mRNA expression of ITGA8 may be a potential diagnosis biomarker and ITGA5 may serve as an independent prognosis indicator for COAD.
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Gong YZ, Ruan GT, Liao XW, Wang XK, Liao C, Wang S and Gao F: Diagnostic and prognostic values of integrin α subfamily mRNA expression in colon adenocarcinoma. Oncol Rep 42: 923-936, 2019.
APA
Gong, Y., Ruan, G., Liao, X., Wang, X., Liao, C., Wang, S., & Gao, F. (2019). Diagnostic and prognostic values of integrin α subfamily mRNA expression in colon adenocarcinoma. Oncology Reports, 42, 923-936. https://doi.org/10.3892/or.2019.7216
MLA
Gong, Y., Ruan, G., Liao, X., Wang, X., Liao, C., Wang, S., Gao, F."Diagnostic and prognostic values of integrin α subfamily mRNA expression in colon adenocarcinoma". Oncology Reports 42.3 (2019): 923-936.
Chicago
Gong, Y., Ruan, G., Liao, X., Wang, X., Liao, C., Wang, S., Gao, F."Diagnostic and prognostic values of integrin α subfamily mRNA expression in colon adenocarcinoma". Oncology Reports 42, no. 3 (2019): 923-936. https://doi.org/10.3892/or.2019.7216
Copy and paste a formatted citation
x
Spandidos Publications style
Gong YZ, Ruan GT, Liao XW, Wang XK, Liao C, Wang S and Gao F: Diagnostic and prognostic values of integrin α subfamily mRNA expression in colon adenocarcinoma. Oncol Rep 42: 923-936, 2019.
APA
Gong, Y., Ruan, G., Liao, X., Wang, X., Liao, C., Wang, S., & Gao, F. (2019). Diagnostic and prognostic values of integrin α subfamily mRNA expression in colon adenocarcinoma. Oncology Reports, 42, 923-936. https://doi.org/10.3892/or.2019.7216
MLA
Gong, Y., Ruan, G., Liao, X., Wang, X., Liao, C., Wang, S., Gao, F."Diagnostic and prognostic values of integrin α subfamily mRNA expression in colon adenocarcinoma". Oncology Reports 42.3 (2019): 923-936.
Chicago
Gong, Y., Ruan, G., Liao, X., Wang, X., Liao, C., Wang, S., Gao, F."Diagnostic and prognostic values of integrin α subfamily mRNA expression in colon adenocarcinoma". Oncology Reports 42, no. 3 (2019): 923-936. https://doi.org/10.3892/or.2019.7216
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