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Article

Hedyotis diffusa Willd. inhibits VEGF‑C‑mediated lymphangiogenesis in colorectal cancer via multiple signaling pathways

  • Authors:
    • Huang Li
    • Zijun Lai
    • Hong Yang
    • Jun Peng
    • Youqin Chen
    • Jiumao Lin
  • View Affiliations / Copyright

    Affiliations: College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China, Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China, Case Western Reserve University School of Medicine, Rainbow Babies and Children's Hospital, Cleveland, OH 44106, USA
  • Pages: 1225-1236
    |
    Published online on: July 5, 2019
       https://doi.org/10.3892/or.2019.7223
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Abstract

Colorectal cancer (CRC) is one of the most commonly diagnosed malignancies worldwide. For patients diagnosed with the presence of metastatic disease, surgery is not suitable for the majority of them. Lymphangiogenesis is a key factor during cancer metastasis and is regulated by vascular endothelial growth factor C (VEGF‑C). Hedyotis diffusa Willd. (HDW) is a Chinese herb of the Rubiaceae family that reportedly inhibits tumor metastasis. However, its underlying anticancer mechanisms have not yet been elucidated. In the present study, we investigated the effects of an ethanol extract of HDW (EEHDW) on the migration capacity by wound healing and Transwell assays, and the effect on the VEGF‑C expression in different CRC cell lines by western blot analysis and ELISA assays. A model of VEGF‑C‑stimulated human lymphatic endothelial cells (HLECs) was constructed. It was found that EEHDW suppressed lymphangiogenesis via the mediation of multiple pathways, which attenuated the migration of cells and their tube formation abilities. Multiple signaling pathways were found to be involved in the VEGF‑C‑mediated lymphangiogenesis. After EEHDW treatment in VEGF‑C‑stimulated HLECs, EEHDW was found to downregulate the expression levels of multiple signaling pathways. Taken together, these results indicate that EEHDW possesses significant anti‑metastatic activities. Moreover, the suppressive effect of EEHDW on lymphangiogenesis, particularly via downregulation of VEGF‑C, partly explains the potential molecular mechanism underlying the inhibitory effect of EEHDW on CRC metastasis.
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Copy and paste a formatted citation
Spandidos Publications style
Li H, Lai Z, Yang H, Peng J, Chen Y and Lin J: Hedyotis diffusa Willd. inhibits VEGF‑C‑mediated lymphangiogenesis in colorectal cancer via multiple signaling pathways. Oncol Rep 42: 1225-1236, 2019.
APA
Li, H., Lai, Z., Yang, H., Peng, J., Chen, Y., & Lin, J. (2019). Hedyotis diffusa Willd. inhibits VEGF‑C‑mediated lymphangiogenesis in colorectal cancer via multiple signaling pathways. Oncology Reports, 42, 1225-1236. https://doi.org/10.3892/or.2019.7223
MLA
Li, H., Lai, Z., Yang, H., Peng, J., Chen, Y., Lin, J."Hedyotis diffusa Willd. inhibits VEGF‑C‑mediated lymphangiogenesis in colorectal cancer via multiple signaling pathways". Oncology Reports 42.3 (2019): 1225-1236.
Chicago
Li, H., Lai, Z., Yang, H., Peng, J., Chen, Y., Lin, J."Hedyotis diffusa Willd. inhibits VEGF‑C‑mediated lymphangiogenesis in colorectal cancer via multiple signaling pathways". Oncology Reports 42, no. 3 (2019): 1225-1236. https://doi.org/10.3892/or.2019.7223
Copy and paste a formatted citation
x
Spandidos Publications style
Li H, Lai Z, Yang H, Peng J, Chen Y and Lin J: Hedyotis diffusa Willd. inhibits VEGF‑C‑mediated lymphangiogenesis in colorectal cancer via multiple signaling pathways. Oncol Rep 42: 1225-1236, 2019.
APA
Li, H., Lai, Z., Yang, H., Peng, J., Chen, Y., & Lin, J. (2019). Hedyotis diffusa Willd. inhibits VEGF‑C‑mediated lymphangiogenesis in colorectal cancer via multiple signaling pathways. Oncology Reports, 42, 1225-1236. https://doi.org/10.3892/or.2019.7223
MLA
Li, H., Lai, Z., Yang, H., Peng, J., Chen, Y., Lin, J."Hedyotis diffusa Willd. inhibits VEGF‑C‑mediated lymphangiogenesis in colorectal cancer via multiple signaling pathways". Oncology Reports 42.3 (2019): 1225-1236.
Chicago
Li, H., Lai, Z., Yang, H., Peng, J., Chen, Y., Lin, J."Hedyotis diffusa Willd. inhibits VEGF‑C‑mediated lymphangiogenesis in colorectal cancer via multiple signaling pathways". Oncology Reports 42, no. 3 (2019): 1225-1236. https://doi.org/10.3892/or.2019.7223
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