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Article Open Access

NAMPT: A potential prognostic and therapeutic biomarker in patients with glioblastoma

  • Authors:
    • Qiuyun Guo
    • Na Han
    • Lei Shi
    • Li Yang
    • Xiaoxi Zhang
    • Yangmei Zhou
    • Shiying Yu
    • Mengxian Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China
    Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 963-972
    |
    Published online on: July 10, 2019
       https://doi.org/10.3892/or.2019.7227
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Abstract

Glioblastoma (GBM) is the most common primary intracranial malignancy. GBM still exhibits high recurrence and mortality rates even following combined treatment with surgery, radiotherapy and chemotherapy, Therefore, the identification of novel therapeutic targets is urgent. Previous research has shown that nicotinamide phosphoribosyltransferase (NAMPT) plays a key role in cell metabolism and is closely related to the occurrence and development of many tumor types; yet, little is known concerning its relationship with GBM. Oncomine database analysis showed that the expression of NAMPT in GBM was higher than that in normal tissues; this finding was further confirmed by immunohistochemical staining of a tissue microarray. Data analysis with the R2 platform showed that patients with higher expression of NAMPT had worse prognoses than those with lower NAMPT expression. Using the GBM data in TCGA, four pathways enriched in the high NAMPT expression group were identified by gene set enrichment analysis (GSEA). NAMPT expression was knocked down in U87 and U251 GBM cells by lentiviral vectors carrying a small hairpin RNA (shRNA) targeting NAMPT. CCK‑8, colony formation, wound healing, Transwell and apoptosis assays were carried out. The results showed that NAMPT knockdown decreased cell proliferation, migration, and invasion and promoted apoptosis. U87 GBM cells were used in a model of subcutaneous tumorigenesis in nude mice. The results showed that NAMPT knockdown slowed the growth of tumors in vivo. Therefore, we speculate that NAMPT may be a potential prognostic and therapeutic biomarker for glioblastoma.
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Copy and paste a formatted citation
Spandidos Publications style
Guo Q, Han N, Shi L, Yang L, Zhang X, Zhou Y, Yu S and Zhang M: NAMPT: A potential prognostic and therapeutic biomarker in patients with glioblastoma. Oncol Rep 42: 963-972, 2019.
APA
Guo, Q., Han, N., Shi, L., Yang, L., Zhang, X., Zhou, Y. ... Zhang, M. (2019). NAMPT: A potential prognostic and therapeutic biomarker in patients with glioblastoma. Oncology Reports, 42, 963-972. https://doi.org/10.3892/or.2019.7227
MLA
Guo, Q., Han, N., Shi, L., Yang, L., Zhang, X., Zhou, Y., Yu, S., Zhang, M."NAMPT: A potential prognostic and therapeutic biomarker in patients with glioblastoma". Oncology Reports 42.3 (2019): 963-972.
Chicago
Guo, Q., Han, N., Shi, L., Yang, L., Zhang, X., Zhou, Y., Yu, S., Zhang, M."NAMPT: A potential prognostic and therapeutic biomarker in patients with glioblastoma". Oncology Reports 42, no. 3 (2019): 963-972. https://doi.org/10.3892/or.2019.7227
Copy and paste a formatted citation
x
Spandidos Publications style
Guo Q, Han N, Shi L, Yang L, Zhang X, Zhou Y, Yu S and Zhang M: NAMPT: A potential prognostic and therapeutic biomarker in patients with glioblastoma. Oncol Rep 42: 963-972, 2019.
APA
Guo, Q., Han, N., Shi, L., Yang, L., Zhang, X., Zhou, Y. ... Zhang, M. (2019). NAMPT: A potential prognostic and therapeutic biomarker in patients with glioblastoma. Oncology Reports, 42, 963-972. https://doi.org/10.3892/or.2019.7227
MLA
Guo, Q., Han, N., Shi, L., Yang, L., Zhang, X., Zhou, Y., Yu, S., Zhang, M."NAMPT: A potential prognostic and therapeutic biomarker in patients with glioblastoma". Oncology Reports 42.3 (2019): 963-972.
Chicago
Guo, Q., Han, N., Shi, L., Yang, L., Zhang, X., Zhou, Y., Yu, S., Zhang, M."NAMPT: A potential prognostic and therapeutic biomarker in patients with glioblastoma". Oncology Reports 42, no. 3 (2019): 963-972. https://doi.org/10.3892/or.2019.7227
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