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Article Open Access

STK31 regulates the proliferation and cell cycle of lung cancer cells via the Wnt/β‑catenin pathway and feedback regulation by c‑myc

  • Authors:
    • Jie Xiong
    • Shigang Xing
    • Zheng Dong
    • Lei Niu
    • Qinghua Xu
    • Pingyi Liu
    • Peixia Yang
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory, Linyi Central Hospital, Yishui, Shandong 276400, P.R. China, Department of Laboratory, Linyi Central Hospital, Yishui, Shandong 276400, P.R. China
    Copyright: © Xiong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 395-404
    |
    Published online on: December 19, 2019
       https://doi.org/10.3892/or.2019.7441
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Abstract

Lung cancer, which is a leading cause of cancer‑related deaths, is diagnosed at a male to female ratio of 2.1:1. Serine‑threonine kinase 31 (STK31) is a novel cancer/testis (CT)‑related gene that is highly expressed in several types of cancers, such as lung and colorectal cancer, and plays crucial roles in cancer. In the present study, increased expression of STK31 and β‑catenin was observed in lung cancer tissues and cell lines. Downregulation of STK31 expression in lung cancer cells significantly inhibited their proliferation by arresting the cell cycle in the G1 phase concurrent with decreased β‑catenin, c‑myc and cyclin D1 protein levels, while upregulation of STK31 had the opposite effects. In addition, STK31‑induced lung cancer cell viability, proliferation, cell cycle progression, and expression of related genes were completely attenuated by a Wnt/β‑catenin inhibitor (XAV939). Similar to XAV939, a c‑myc inhibitor (10058‑F4) also significantly attenuated STK31‑induced proliferation and cell cycle progression in lung cancer cells. Inhibiting c‑myc and TRRAP significantly decreased the expression of STK31, and a chromatin immunoprecipitation (ChIP) assay confirmed that c‑myc directly bound to the STK31 promoter. These results indicated that STK31 may act as an oncogene in lung cancer and that c‑myc may be the transcription factor that promotes STK31 expression. Moreover, the results suggested that c‑myc can also regulate STK31 expression in a positive feedback loop, and the downregulation of STK31 in lung cancer cells had an inhibitory effect on cell viability, cell proliferation and cell cycle progression, likely by inactivating the Wnt/β‑catenin pathway and positive feedback regulation by c‑myc.
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Copy and paste a formatted citation
Spandidos Publications style
Xiong J, Xing S, Dong Z, Niu L, Xu Q, Liu P and Yang P: STK31 regulates the proliferation and cell cycle of lung cancer cells via the Wnt/β‑catenin pathway and feedback regulation by c‑myc. Oncol Rep 43: 395-404, 2020.
APA
Xiong, J., Xing, S., Dong, Z., Niu, L., Xu, Q., Liu, P., & Yang, P. (2020). STK31 regulates the proliferation and cell cycle of lung cancer cells via the Wnt/β‑catenin pathway and feedback regulation by c‑myc. Oncology Reports, 43, 395-404. https://doi.org/10.3892/or.2019.7441
MLA
Xiong, J., Xing, S., Dong, Z., Niu, L., Xu, Q., Liu, P., Yang, P."STK31 regulates the proliferation and cell cycle of lung cancer cells via the Wnt/β‑catenin pathway and feedback regulation by c‑myc". Oncology Reports 43.2 (2020): 395-404.
Chicago
Xiong, J., Xing, S., Dong, Z., Niu, L., Xu, Q., Liu, P., Yang, P."STK31 regulates the proliferation and cell cycle of lung cancer cells via the Wnt/β‑catenin pathway and feedback regulation by c‑myc". Oncology Reports 43, no. 2 (2020): 395-404. https://doi.org/10.3892/or.2019.7441
Copy and paste a formatted citation
x
Spandidos Publications style
Xiong J, Xing S, Dong Z, Niu L, Xu Q, Liu P and Yang P: STK31 regulates the proliferation and cell cycle of lung cancer cells via the Wnt/β‑catenin pathway and feedback regulation by c‑myc. Oncol Rep 43: 395-404, 2020.
APA
Xiong, J., Xing, S., Dong, Z., Niu, L., Xu, Q., Liu, P., & Yang, P. (2020). STK31 regulates the proliferation and cell cycle of lung cancer cells via the Wnt/β‑catenin pathway and feedback regulation by c‑myc. Oncology Reports, 43, 395-404. https://doi.org/10.3892/or.2019.7441
MLA
Xiong, J., Xing, S., Dong, Z., Niu, L., Xu, Q., Liu, P., Yang, P."STK31 regulates the proliferation and cell cycle of lung cancer cells via the Wnt/β‑catenin pathway and feedback regulation by c‑myc". Oncology Reports 43.2 (2020): 395-404.
Chicago
Xiong, J., Xing, S., Dong, Z., Niu, L., Xu, Q., Liu, P., Yang, P."STK31 regulates the proliferation and cell cycle of lung cancer cells via the Wnt/β‑catenin pathway and feedback regulation by c‑myc". Oncology Reports 43, no. 2 (2020): 395-404. https://doi.org/10.3892/or.2019.7441
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