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Article

Cold atmospheric plasma‑activated Ringer's solution inhibits the proliferation of osteosarcoma cells through the mitochondrial apoptosis pathway

  • Authors:
    • Shenglin Xu
    • Yuan Wang
    • Yukang Que
    • Chengxiao Ma
    • Shang Cai
    • Hao Wang
    • Xingyu Yang
    • Chunjun Yang
    • Cheng Cheng
    • Guoping Zhao
    • Yong Hu
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, The First Affiliated Hospital, Anhui Medical University, Hefei, Anhui 230000, P.R. China, Department of Dermatology, The Second Affiliated Hospital, Anhui Medical University, Hefei, Anhui 230000, P.R. China, The Institute of Plasma Physics, Chinese Academy of Science, Hefei, Anhui 230000, P.R. China, Anhui Province Key Laboratory of Environmental Toxicology and Pollution Control Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui 230000, P.R. China
  • Pages: 1683-1691
    |
    Published online on: February 25, 2020
       https://doi.org/10.3892/or.2020.7518
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Abstract

The present study aimed to investigate the effects of cold atmospheric plasma (CAP)‑activated Ringer's solution on osteosarcoma cell lines MG63 and U2OS, and to identify the molecular mechanism underlying these effects. CAP‑activated Ringer's solution was used to treat osteosarcoma cell lines MG63 and U2OS for 30 min. Cell viability was measured using the MTT method. The apoptosis rate was detected using Annexin‑V and propidium iodide. The expression levels of cytochrome c, caspase‑3 and polyADP ribose polymerase (PARP) in MG63 cells were analyzed via western blotting. The change in mitochondrial membrane potential was detected via the JC‑1 dye method and verified by the level of reactive oxygen species (ROS). CAP‑activated Ringer's solution inhibited the proliferation of MG63 and U2OS cells in a dose‑ and time‑dependent manner. Furthermore, CAP‑activated Ringer's solution induced the apoptosis of MG63 cells, increased the intracellular ROS level, decreased the mitochondrial membrane potential level, and induced the release of cytochrome c. CAP‑activated Ringer's solution inhibits osteosarcoma cell proliferation through intracellular ROS‑mediated mitochondrial apoptosis.
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View References

1 

Geller DS and Gorlick R: Osteosarcoma: A review of diagnosis, management, and treatment strategies. Clin Adv Hematol Oncol. 8:705–718. 2010.PubMed/NCBI

2 

Blattmann C, Oertel S, Schulz-Ertner D, Rieken S, Haufe S, Ewerbeck V, Unterberg A, Karapanagiotou-Schenkel I, Combs SE, Nikoghosyan A, et al: Non-randomized therapy trial to determine the safety and efficacy of heavy ion radiotherapy in patients with non-resectable osteosarcoma. BMC Cancer. 10:962010. View Article : Google Scholar : PubMed/NCBI

3 

Luetke A, Meyers PA, Lewis I and Juergens H: Osteosarcoma treatment-where do we stand? A state of the art review. Cancer Treat Rev. 40:523–532. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Bacci G, Longhi A, Versari M, Mercuri M, Briccoli A and Picci P: Prognostic factors for osteosarcoma of the extremity treated with neoadjuvant chemotherapy: 15-year experience in 789 patients treated at a single institution. Cancer. 106:1154–1161. 2006. View Article : Google Scholar : PubMed/NCBI

5 

Wang W, Yang J, Wang Y, Wang D, Han G, Jia J, Xu M and Bi W: Survival and prognostic factors in Chinese patients with osteosarcoma: 13-year experience in 365 patients treated at a single institution. Pathol Res Pract. 213:119–125. 2017. View Article : Google Scholar : PubMed/NCBI

6 

Yan D, Sherman JH and Keidar M: Cold atmospheric plasma, a novel promising anti-cancer treatment modality. Oncotarget. 8:15977–15995. 2017.PubMed/NCBI

7 

Keidar M, Yan D, Beilis II, Trink B and Sherman JH: Plasmas for treating cancer: Opportunities for adaptive and self-adaptive approaches. Trends Biotechnol. 36:586–593. 2018. View Article : Google Scholar : PubMed/NCBI

8 

Lee HJ, Shon CH, Kim YS, Kim S, Kim GC and Kong MG: Degradation of adhesion molecules of G361 melanoma cells by a non-thermal atmospheric pressure microplasma. New J Phys. 11:1150262009. View Article : Google Scholar

9 

Schmidt A and Bekeschus S: Redox for repair: Cold physical plasmas and Nrf2 signaling promoting wound healing. Antioxidants (Basel). 7(pii): E1462018. View Article : Google Scholar : PubMed/NCBI

10 

Iseki S, Ohta T, Aomatsu A, Ito M, Kano H, Higashijima Y and Hori M: Rapid inactivation of penicillium digitatum spores using high-density nonequilibrium atmospheric pressure plasma. Appl Phys Lett. 96:1537042010. View Article : Google Scholar : PubMed/NCBI

11 

Calvo T, Alvarez-Ordóñez A, Prieto M, Bernardo A and López M: Stress adaptation has a minor impact on the effectivity of non-thermal atmospheric plasma (NTAP) against salmonella spp. Food Res Int. 102:519–525. 2017. View Article : Google Scholar : PubMed/NCBI

12 

Köritzer J, Boxhammer V, Schäfer A, Shimizu T, Klämpfl TG, Li YF, Welz C, Schwenk-Zieger S, Morfill GE, Zimmermann JL and Schlegel J: Restoration of sensitivity in chemo-resistant glioma cells by cold atmospheric plasma. PLoS One. 8:e644982013. View Article : Google Scholar : PubMed/NCBI

13 

Chauvin J, Gibot L, Griseti E, Golzio M, Rols MP, Merbahi N and Vicendo P: Elucidation of in vitro cellular steps induced by antitumor treatment with plasma-activated medium. Sci Rep. 9:48662019. View Article : Google Scholar : PubMed/NCBI

14 

Boehm D, Curtin J, Cullen PJ and Bourke P: Hydrogen peroxide and beyond-the potential of high-voltage plasma-activated liquids against cancerous cells. Anticancer Agents Med Chem. 18:815–823. 2018. View Article : Google Scholar : PubMed/NCBI

15 

Yan D, Cui H, Zhu W, Nourmohammadi N, Milberg J, Zhang LG, Sherman JH and Keidar M: The specific vulnerabilities of cancer cells to the cold atmospheric plasma-stimulated solutions. Sci Rep. 7:44792017. View Article : Google Scholar : PubMed/NCBI

16 

Xiang L, Xu X, Zhang S, Cai D and Dai X: Cold atmospheric plasma conveys selectivity on triple negative breast cancer cells both in vitro and in vivo. Free Radic Biol Med. 124:205–213. 2018. View Article : Google Scholar : PubMed/NCBI

17 

Utsumi F, Kajiyama H, Nakamura K, Tanaka H, Mizuno M, Ishikawa K, Kondo H, Kano H, Hori M and Kikkawa F: Effect of indirect nonequilibrium atmospheric pressure plasma on anti-proliferative activity against chronic chemo-resistant ovarian cancer cells in vitro and in vivo. PLoS One. 8:e815762013. View Article : Google Scholar : PubMed/NCBI

18 

Bekeschus S, Käding A, Schröder T, Wende K, Hackbarth C, Liedtke KR, van der Linde J, von Woedtke T, Heidecke CD and Partecke LI: Cold physical plasma-treated buffered saline solution as effective agent against pancreatic cancer cells. Anticancer Agents Med Chem. 18:824–831. 2018. View Article : Google Scholar : PubMed/NCBI

19 

Wang L, Yang X, Yang C, Gao J, Zhao Y, Cheng C, Zhao G and Liu S: The inhibition effect of cold atmospheric plasma-activated media in cutaneous squamous carcinoma cells. Future Oncol. 15:495–505. 2019. View Article : Google Scholar : PubMed/NCBI

20 

Tanaka H, Nakamura K, Mizuno M, Ishikawa K, Takeda K, Kajiyama H, Utsumi F, Kikkawa F and Hori M: Non-thermal atmospheric pressure plasma activates lactate in Ringer's solution for anti-tumor effects. Sci Rep. 6:362822016. View Article : Google Scholar : PubMed/NCBI

21 

Judée F, Fongia C, Ducommun B, Yousfi M, Lobjois V and Merbahi N: Short and long time effects of low temperature plasma activated media on 3D multicellular tumor spheroids. Sci Rep. 6:214212016. View Article : Google Scholar : PubMed/NCBI

22 

Cooper M, Fridman G, Staack D, Gutsol AF, Vasilets VN, Anandan S, Cho YI, Fridman A and Tsapin A: Decontamination of surfaces from extremophile organisms using nonthermal atmospheric-pressure plasmas. IEEE Trans Plasma Sci. 37:866–871. 2009. View Article : Google Scholar

23 

Chauvin J, Judée F, Yousfi M, Vicendo P and Merbahi N: Analysis of reactive oxygen and nitrogen species generated in three liquid media by low temperature helium plasma jet. Sci Rep. 7:45622017. View Article : Google Scholar : PubMed/NCBI

24 

Ruan Z, Guo Y, Gao J, Yang C, Lan Y, Shen J, Xu Z, Cheng C, Liu X, Zhang S, et al: Control of multidrug-resistant planktonic Acinetobacter baumannii: Biocidal efficacy study by atmospheric-pressure air plasma. Plasma Sci Technol. 20:0655132018. View Article : Google Scholar

25 

Liu Y, Tan S, Zhang H, Kong X, Ding L, Shen J, Lan Y, Cheng C, Zhu T and Xia W: Selective effects of non-thermal atmospheric plasma on triple-negative breast normal and carcinoma cells through different cell signaling pathways. Sci Rep. 7:79802017. View Article : Google Scholar : PubMed/NCBI

26 

Ottaviani G, Robert RS, Huh WW, Palla S and Jaffe N: Sociooccupational and physical outcomes more than 20 years after the diagnosis of osteosarcoma in children and adolescents: Limb salvage versus amputation. Cancer. 119:3727–3736. 2013. View Article : Google Scholar : PubMed/NCBI

27 

Mavrogenis AF, Abati CN, Romagnoli C and Ruggieri P: Similar survival but better function for patients after limb salvage versus amputation for distal tibia osteosarcoma. Clin Orthop Relat Res. 470:1735–1748. 2012. View Article : Google Scholar : PubMed/NCBI

28 

Takeuchi A, Lewis VO, Satcher RL, Moon BS and Lin PP: What are the factors that affect survival and relapse after local recurrence of osteosarcoma? Clin Orthop Relat Res. 472:3188–3195. 2014. View Article : Google Scholar : PubMed/NCBI

29 

Guerrero-Preston R, Ogawa T, Uemura M, Shumulinsky G, Valle BL, Pirini F, Ravi R, Sidransky D, Keidar M and Trink B: Cold atmospheric plasma treatment selectively targets head and neck squamous cell carcinoma cells. Int J Mol Med. 34:941–946. 2014. View Article : Google Scholar : PubMed/NCBI

30 

Schuster M, Seebauer C, Rutkowski R, Hauschild A, Podmelle F, Metelmann C, Metelmann B, von Woedtke T, Hasse S, Weltmann KD and Metelmann HR: Visible tumor surface response to physical plasma and apoptotic cell kill in head and neck cancer. J Craniomaxillofac Surg. 44:1445–1452. 2016. View Article : Google Scholar : PubMed/NCBI

31 

Koensgen D, Besic I, Gümbel D, Kaul A, Weiss M, Diesing K, Kramer A, Bekeschus S, Mustea A and Stope MB: Cold atmospheric plasma (CAP) and CAP-stimulated cell culture media suppress ovarian cancer cell growth-A putative treatment option in ovarian cancer therapy. Anticancer Res. 37:6739–6744. 2017.PubMed/NCBI

32 

Yan D, Talbot A, Nourmohammadi N, Cheng X, Canady J, Sherman J and Keidar M: Principles of using cold atmospheric plasma stimulated media for cancer treatment. Sci Rep. 5:183392015. View Article : Google Scholar : PubMed/NCBI

33 

Chen Z, Li L, Cheng X, Gjika E and Keidar M: Effects of cold atmospheric plasma generated in deionized water in cell cancer therapy. Plasma Process Polym. 13:1151–1156. 2016. View Article : Google Scholar

34 

Li XY, Feng Z, Pu SC, Yun Y, Shi XM and Xu Z: Cold atmospheric plasma jet-generated oxidized derivatives of tryptophan and their selective effects on murine melanoma and fibroblast cells. Plasma Chem Plasma Process. 38:919–936. 2018. View Article : Google Scholar

35 

Qiao J, Wu Y, Liu Y, Li X, Wu X, Liu N, Zhu F, Qi K, Cheng H, Li D, et al: Busulfan triggers intrinsic mitochondrial-dependent platelet apoptosis independent of platelet activation. Biol Blood Marrow Transplant. 22:1565–1572. 2016. View Article : Google Scholar : PubMed/NCBI

36 

Lemeshko VV: VDAC electronics: 3. VDAC-Creatine kinase-dependent generation of the outer membrane potential in respiring mitochondria. Biochim Biophys Acta. 1858:1411–1418. 2016. View Article : Google Scholar : PubMed/NCBI

37 

Lemeshko VV: VDAC electronics: 5 Mechanism and computational model of hexokinase-dependent generation of the outer membrane potential in brain mitochondria. Biochim Biophys Acta Biomembr. 1860:2599–2607. 2018. View Article : Google Scholar : PubMed/NCBI

38 

Elefantova K, Lakatos B, Kubickova J, Sulova Z and Breier A: Detection of the mitochondrial membrane potential by the cationic dye JC-1 in L1210 cells with massive overexpression of the plasma membrane ABCB1 drug transporter. Int J Mol Sci. 19(pii): E19852018. View Article : Google Scholar : PubMed/NCBI

39 

Brooks MM, Neelam S, Fudala R, Gryczynski I and Cammarata PR: Lenticular mitoprotection. Part A: Monitoring mitochondrial depolarization with JC-1 and artifactual fluorescence by the glycogen synthase kinase-3β inhibitor, SB216763. Mol Vis. 19:1406–1412. 2013.PubMed/NCBI

40 

Haeberlein SL: Mitochondrial function in apoptotic neuronal cell death. Neurochem Res. 29:521–530. 2004. View Article : Google Scholar : PubMed/NCBI

41 

Zorov DB, Juhaszova M and Sollott SJ: Mitochondrial reactive oxygen species (ROS) and ROS-induced ROS release. Physiol Rev. 94:909–950. 2014. View Article : Google Scholar : PubMed/NCBI

42 

Lee HS, Hwang CY, Shin SY, Kwon KS and Cho KH: MLK3 is part of a feedback mechanism that regulates different cellular responses to reactive oxygen species. Sci Signal. 7:ra522014. View Article : Google Scholar : PubMed/NCBI

43 

Song HJ, Lee EK, Lee JA, Kim HL and Jang KW: The addition of mifamurtide to chemotherapy improves lifetime effectiveness in children with osteosarcoma: A Markov model analysis. Tumour Biol. 35:8771–8779. 2014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Xu S, Wang Y, Que Y, Ma C, Cai S, Wang H, Yang X, Yang C, Cheng C, Zhao G, Zhao G, et al: Cold atmospheric plasma‑activated Ringer's solution inhibits the proliferation of osteosarcoma cells through the mitochondrial apoptosis pathway. Oncol Rep 43: 1683-1691, 2020.
APA
Xu, S., Wang, Y., Que, Y., Ma, C., Cai, S., Wang, H. ... Hu, Y. (2020). Cold atmospheric plasma‑activated Ringer's solution inhibits the proliferation of osteosarcoma cells through the mitochondrial apoptosis pathway. Oncology Reports, 43, 1683-1691. https://doi.org/10.3892/or.2020.7518
MLA
Xu, S., Wang, Y., Que, Y., Ma, C., Cai, S., Wang, H., Yang, X., Yang, C., Cheng, C., Zhao, G., Hu, Y."Cold atmospheric plasma‑activated Ringer's solution inhibits the proliferation of osteosarcoma cells through the mitochondrial apoptosis pathway". Oncology Reports 43.5 (2020): 1683-1691.
Chicago
Xu, S., Wang, Y., Que, Y., Ma, C., Cai, S., Wang, H., Yang, X., Yang, C., Cheng, C., Zhao, G., Hu, Y."Cold atmospheric plasma‑activated Ringer's solution inhibits the proliferation of osteosarcoma cells through the mitochondrial apoptosis pathway". Oncology Reports 43, no. 5 (2020): 1683-1691. https://doi.org/10.3892/or.2020.7518
Copy and paste a formatted citation
x
Spandidos Publications style
Xu S, Wang Y, Que Y, Ma C, Cai S, Wang H, Yang X, Yang C, Cheng C, Zhao G, Zhao G, et al: Cold atmospheric plasma‑activated Ringer's solution inhibits the proliferation of osteosarcoma cells through the mitochondrial apoptosis pathway. Oncol Rep 43: 1683-1691, 2020.
APA
Xu, S., Wang, Y., Que, Y., Ma, C., Cai, S., Wang, H. ... Hu, Y. (2020). Cold atmospheric plasma‑activated Ringer's solution inhibits the proliferation of osteosarcoma cells through the mitochondrial apoptosis pathway. Oncology Reports, 43, 1683-1691. https://doi.org/10.3892/or.2020.7518
MLA
Xu, S., Wang, Y., Que, Y., Ma, C., Cai, S., Wang, H., Yang, X., Yang, C., Cheng, C., Zhao, G., Hu, Y."Cold atmospheric plasma‑activated Ringer's solution inhibits the proliferation of osteosarcoma cells through the mitochondrial apoptosis pathway". Oncology Reports 43.5 (2020): 1683-1691.
Chicago
Xu, S., Wang, Y., Que, Y., Ma, C., Cai, S., Wang, H., Yang, X., Yang, C., Cheng, C., Zhao, G., Hu, Y."Cold atmospheric plasma‑activated Ringer's solution inhibits the proliferation of osteosarcoma cells through the mitochondrial apoptosis pathway". Oncology Reports 43, no. 5 (2020): 1683-1691. https://doi.org/10.3892/or.2020.7518
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