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Systematic analysis of lysine acetylome and succinylome reveals the correlation between modification of H2A.X complexes and DNA damage response in breast cancer

  • Authors:
    • Xiuli Gao
    • Hongguang Bao
    • Likun Liu
    • Wenbin Zhu
    • Liping Zhang
    • Liling Yue
  • View Affiliations / Copyright

    Affiliations: Research Institute of Medicine and Pharmacy, Qiqihar Medical University, Qiqihar, Heilongjiang 161006, P.R. China, Oncology Surgical Department, The Second Affiliated Hospital of Qiqihar Medical University, Qiqihar, Heilongjiang 161006, P.R. China, Department of Medical Technology, Qiqihar Medical University, Qiqihar, Heilongjiang 161006, P.R. China
    Copyright: © Gao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1819-1830
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    Published online on: March 19, 2020
       https://doi.org/10.3892/or.2020.7554
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Abstract

Abnormal protein acetylation and succinylation in lysine residues can cause the initiation and development of numerous different types of tumors. However, to the best of our knowledge, there is currently a lack of systematic investigation in breast cancer. Using proteomic techniques, the present study systematically investigated the two modifications of all proteins in invasive ductal carcinoma tissues to identify potential targets. The results revealed significantly higher modification levels for the majority of proteins in breast cancer tissue when compared with para‑carcinomous normal tissue. The bioinformatic analysis demonstrated that either highly acetylated or succinylated proteins were significantly enriched in histone H2A.X (H2A.X) complexes and nucleophosmin (NPM1) may be the key member among them. The results of further analyses revealed that H2A.X complexes were associated with DNA damage response (DDR), and the proteomic results for protein quantification provided further evidence for the abnormal DDR condition in breast cancer tissues. Later, the western blotting results validated the high acetylation and succinylation levels of the majority of proteins, including the modification of NPM1 and its correlation with cell viability. Finally, the upregulation of H2A.X in breast cancer tissues further demonstrated the association between H2A.X complex modification and DDR in breast cancer. Overall, the present study systematically investigated the protein acetylation and succinylation in breast cancer and provided evidence to support H2A.X complexes as potential targets. These results broaden the horizon for breast cancer investigation and link it with epigenetics.
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Copy and paste a formatted citation
Spandidos Publications style
Gao X, Bao H, Liu L, Zhu W, Zhang L and Yue L: Systematic analysis of lysine acetylome and succinylome reveals the correlation between modification of H2A.X complexes and DNA damage response in breast cancer. Oncol Rep 43: 1819-1830, 2020.
APA
Gao, X., Bao, H., Liu, L., Zhu, W., Zhang, L., & Yue, L. (2020). Systematic analysis of lysine acetylome and succinylome reveals the correlation between modification of H2A.X complexes and DNA damage response in breast cancer. Oncology Reports, 43, 1819-1830. https://doi.org/10.3892/or.2020.7554
MLA
Gao, X., Bao, H., Liu, L., Zhu, W., Zhang, L., Yue, L."Systematic analysis of lysine acetylome and succinylome reveals the correlation between modification of H2A.X complexes and DNA damage response in breast cancer". Oncology Reports 43.6 (2020): 1819-1830.
Chicago
Gao, X., Bao, H., Liu, L., Zhu, W., Zhang, L., Yue, L."Systematic analysis of lysine acetylome and succinylome reveals the correlation between modification of H2A.X complexes and DNA damage response in breast cancer". Oncology Reports 43, no. 6 (2020): 1819-1830. https://doi.org/10.3892/or.2020.7554
Copy and paste a formatted citation
x
Spandidos Publications style
Gao X, Bao H, Liu L, Zhu W, Zhang L and Yue L: Systematic analysis of lysine acetylome and succinylome reveals the correlation between modification of H2A.X complexes and DNA damage response in breast cancer. Oncol Rep 43: 1819-1830, 2020.
APA
Gao, X., Bao, H., Liu, L., Zhu, W., Zhang, L., & Yue, L. (2020). Systematic analysis of lysine acetylome and succinylome reveals the correlation between modification of H2A.X complexes and DNA damage response in breast cancer. Oncology Reports, 43, 1819-1830. https://doi.org/10.3892/or.2020.7554
MLA
Gao, X., Bao, H., Liu, L., Zhu, W., Zhang, L., Yue, L."Systematic analysis of lysine acetylome and succinylome reveals the correlation between modification of H2A.X complexes and DNA damage response in breast cancer". Oncology Reports 43.6 (2020): 1819-1830.
Chicago
Gao, X., Bao, H., Liu, L., Zhu, W., Zhang, L., Yue, L."Systematic analysis of lysine acetylome and succinylome reveals the correlation between modification of H2A.X complexes and DNA damage response in breast cancer". Oncology Reports 43, no. 6 (2020): 1819-1830. https://doi.org/10.3892/or.2020.7554
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