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Article Open Access

High‑throughput and continuous flow isolation of rare circulating tumor cells and clusters in gastric cancer from human whole blood samples using electromagnetic vibration‑based filtration

  • Authors:
    • An Xiang
    • Mei Xue
    • Fengling Ren
    • Li Wang
    • Zichen Ye
    • Da Li
    • Qifeng Ji
    • Gang Ji
    • Zifan Lu
  • View Affiliations / Copyright

    Affiliations: State Key Laboratory of Cancer Biology, Department of Biopharmaceutics, School of Pharmacy, Air Force Medical University, Xi'an, Shaanxi 710032, P.R. China, Center for Translational Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China, School of Public Health, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710061, P.R. China, Xijing Hospital of Digestive Diseases, Xijing Hospital, Air Force Medical University, Xi'an, Shaanxi 710032, P.R. China
    Copyright: © Xiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1975-1985
    |
    Published online on: March 30, 2020
       https://doi.org/10.3892/or.2020.7567
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Abstract

Circulating tumor cells (CTCs) or CTC clusters are considered as suitable and relevant targets for liquid biopsy as they more accurately indicate cancer progression, the therapeutic effects of treatment and allows for monitoring of cancer metastasis in real‑time. Among the various methods for isolating CTCs, size‑based filtration is one of the most convenient methods. However, cell clogging makes the filtration process less efficient. In the present study, an electromagnetic vibration‑based filtration (eVBF) device was developed that efficiently isolated rare CTCs and CTC clusters from clinical blood samples of patients with gastric cancer. Using human blood samples spiked with human gastric cancer cells, the parameters of this device such as vibrating amplitude and flow rate were optimized. Putative CTCs were detected using a conventional filtration method and the eVBF device from the peripheral blood samples of patients with gastric cancer. Continuous flow isolation of CTCs was evaluated by a simulated blood flow system. The eVBF device utilized the electromagnetic force to generate a periodic vibration that prevented the cell clogging and improved the filtering efficiency. The optimized eVBF device with the high‑amplitude vibration exhibited a recovery efficiency of 80‑90% from whole blood samples spiked with 100 or 1,000 gastric cancer cells per ml. Using the eVBF device, CTCs were detected in 100% of patients (10/10) with gastric cancer, and the positive detection rate of the eVBF device was 30% higher compared with the conventional filtration method. Furthermore, CTC clusters were detected in 40% (4/10) of CTC‑positive patient samples, and the integrity of CTC clusters was preserved using the eVBF device. The eVBF device allowed for high‑throughput (1 ml/min) and continuous flow isolation of CTCs without the addition of any antibodies, any chemical reagents or any pretreatment processes. Thus, the eVBF device provides an efficient tool for isolating rare CTCs and CTC clusters from patients with cancer, highlighting its potential for use in cancer diagnosis, treatment and cancer biology research.
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View References

1 

Siegel RL, Miller KD and Jemal A: Cancer statistics, 2019. CA Cancer J Clin. 69:7–34. 2019. View Article : Google Scholar

2 

Ortiz V and Yu M: Analyzing circulating tumor cells one at a time. Trends Cell Biol. 28:764–775. 2018. View Article : Google Scholar :

3 

Wang S, Liu K, Liu J, Yu ZT, Xu X, Zhao L, Lee T, Lee EK, Reiss J, Lee YK, et al: Highly efficient capture of circulating tumor cells by using nanostructured silicon substrates with integrated chaotic micromixers. Angew Chem Int Ed Engl. 50:3084–3088. 2011. View Article : Google Scholar :

4 

Alix-Panabières C and Pantel K: Challenges in circulating tumour cell research. Nat Rev Cancer. 14:623–631. 2014. View Article : Google Scholar

5 

Aceto N, Bardia A, Miyamoto DT, Donaldson MC, Wittner BS, Spencer JA, Yu M, Pely A, Engstrom A, Zhu H, et al: Circulating tumor cell clusters are oligoclonal precursors of breast cancer metastasis. Cell. 158:1110–1122. 2014. View Article : Google Scholar :

6 

Hou JM, Krebs MG, Lancashire L, Sloane R, Backen A, Swain RK, Priest LJ, Greystoke A, Zhou C, Morris K, et al: Clinical significance and molecular characteristics of circulating tumor cells and circulating tumor microemboli in patients with small-cell lung cancer. J Clin Oncol. 30:525–532. 2012. View Article : Google Scholar

7 

Giuliano M, Shaikh A, Lo HC, Arpino G, De Placido S, Zhang XH, Cristofanilli M, Schiff R and Trivedi MV: Perspective on circulating tumor cell clusters: Why it takes a village to metastasize. Cancer Res. 78:845–852. 2018. View Article : Google Scholar

8 

Chikaishi Y, Yoneda K, Ohnaga T and Tanaka F: EpCAM-independent capture of circulating tumor cells with a ‘universal CTC-chip’. Oncol Rep. 37:77–82. 2017. View Article : Google Scholar

9 

Abdallah EA, Braun AC, Flores BCTCP, Senda L, Urvanegia AC, Calsavara V, Fonseca de Jesus VH, Almeida MFA, Begnami MD, Coimbra FJF, et al: The potential clinical implications of circulating tumor cells and circulating tumor microemboli in gastric cancer. Oncologist. 24:e854–e863. 2019. View Article : Google Scholar :

10 

Chen JY, Tsai WS, Shao HJ, Wu JC, Lai JM, Lu SH, Hung TF, Yang CT, Wu LC, Chen JS, et al: Sensitive and specific biomimetic lipid coated microfluidics to isolate viable circulating tumor cells and microemboli for cancer detection. PLoS One. 11:e01496332016. View Article : Google Scholar :

11 

Warkiani ME, Khoo BL, Wu L, Tay AK, Bhagat AA, Han J and Lim CT: Ultra-fast, label-free isolation of circulating tumor cells from blood using spiral microfluidics. Nat Protoc. 11:134–148. 2016. View Article : Google Scholar

12 

Kaiser J: Medicine. Cancer's circulation problem. Science. 327:1072–1074. 2010. View Article : Google Scholar

13 

Yu M, Bardia A, Wittner BS, Stott SL, Smas ME, Ting DT, Isakoff SJ, Ciciliano JC, Wells MN, Shah AM, et al: Circulating breast tumor cells exhibit dynamic changes in epithelial and mesenchymal composition. Science. 339:580–584. 2013. View Article : Google Scholar :

14 

Sarioglu AF, Aceto N, Kojic N, Donaldson MC, Zeinali M, Hamza B, Engstrom A, Zhu H, Sundaresan TK, Miyamoto DT, et al: A microfluidic device for label-free, physical capture of circulating tumor cell clusters. Nat Methods. 12:685–691. 2015. View Article : Google Scholar :

15 

Warkiani ME, Guan G, Luan KB, Lee WC, Bhagat AA, Chaudhuri PK, Tan DS, Lim WT, Lee SC, Chen PC, et al: Slanted spiral microfluidics for the ultra-fast, label-free isolation of circulating tumor cells. Lab Chip. 14:128–137. 2014. View Article : Google Scholar

16 

Li M and Anand RK: High-throughput selective capture of single circulating tumor cells by dielectrophoresis at a wireless electrode array. J Am Chem Soc. 139:8950–8959. 2017. View Article : Google Scholar

17 

Wang K, Zhou L, Zhao S, Cheng Z, Qiu S, Lu Y, Wu Z, Abdel Wahab AHA, Mao H and Zhao J: A microfluidic platform for high-purity separating circulating tumor cells at the single-cell level. Talanta. 200:169–176. 2019. View Article : Google Scholar

18 

Vona G, Sabile A, Louha M, Sitruk V, Romana S, Schütze K, Capron F, Franco D, Pazzagli M, Vekemans M, et al: Isolation by size of epithelial tumor cells: A new method for the immunomorphological and molecular characterization of circulatingtumor cells. Am J Pathol. 156:57–63. 2000. View Article : Google Scholar :

19 

Hao SJ, Wan Y, Xia YQ, Zou X and Zheng SY: Size-based separation methods of circulating tumor cells. Adv Drug Deliv Rev. 125:3–20. 2018. View Article : Google Scholar

20 

Farace F, Massard C, Vimond N, Drusch F, Jacques N, Billiot F, Laplanche A, Chauchereau A, Lacroix L, Planchard D, et al: A direct comparison of CellSearch and ISET for circulating tumour-cell detection in patients with metastatic carcinomas. Br J Cancer. 105:847–853. 2011. View Article : Google Scholar :

21 

De Giorgi V, Pinzani P, Salvianti F, Panelos J, Paglierani M, Janowska A, Grazzini M, Wechsler J, Orlando C, Santucci M, et al: Application of a filtration- and isolation-by-size technique for the detection of circulating tumor cells in cutaneous melanoma. J Invest Dermatol. 130:2440–2447. 2010. View Article : Google Scholar

22 

Coumans FA, van Dalum G, Beck M and Terstappen LW: Filter characteristics influencing circulating tumor cell enrichment from whole blood. PLoS One. 8:e617702013. View Article : Google Scholar :

23 

Shapiro HM, Schildkraut ER, Curbelo R, Laird CW, Turner B and Hirschfeld T: Combined blood cell counting and classification with fluorochrome stains and flow instrumentation. J Histochem Cytochem. 24:396–401. 1976. View Article : Google Scholar

24 

Li P, Mao Z, Peng Z, Zhou L, Chen Y, Huang PH, Truica CI, Drabick JJ, El-Deiry WS, Dao M, et al: Acoustic separation of circulating tumor cells. Proc Natl Acad Sci USA. 112:4970–4975. 2015. View Article : Google Scholar

25 

Galanzha EI, Menyaev YA, Yadem AC, Sarimollaoglu M, Juratli MA, Nedosekin DA, Foster SR, Jamshidi-Parsian A, Siegel ER, Makhoul I, et al: In vivo liquid biopsy using cytophone platform for photoacoustic detection of circulating tumor cells in patients with melanoma. Sci Transl Med. 11(pii): eaat58572019. View Article : Google Scholar

26 

Saucedo-Zeni N, Mewes S, Niestroj R, Gasiorowski L, Murawa D, Nowaczyk P, Tomasi T, Weber E, Dworacki G, Morgenthaler NG, et al: A novel method for the in vivo isolation of circulating tumor cells from peripheral blood of cancer patients using a functionalized and structured medical wire. Int J Oncol. 41:1241–1250. 2012.

27 

Zheng S, Lin H, Liu JQ, Balic M, Datar R, Cote RJ and Tai YC: Membrane microfilter device for selective capture, electrolysis and genomic analysis of human circulating tumor cells. J Chromatogr A. 1162:154–161. 2007. View Article : Google Scholar

28 

Zheng S, Lin HK, Lu B, Williams A, Datar R, Cote RJ and Tai YC: 3D microfilter device for viable circulating tumor cell (CTC) enrichment from blood. Biomed Microdevices. 13:203–213. 2011. View Article : Google Scholar

29 

Lin E, Cao T, Nagrath S and King MR: Circulating tumor cells: Diagnostic and therapeutic applications. Annu Rev Biomed Eng. 20:329–352. 2018. View Article : Google Scholar

30 

Iliescu FS, Poenar DP, Yu F, Ni M, Chan KH, Cima I, Taylor HK, Cima I and Iliescu C: Recent advances in microfluidic methods in cancer liquid biopsy. Biomicrofluidics. 13:0415032019. View Article : Google Scholar

31 

Zheng X, Fan L, Zhou P, Ma H, Huang S, Yu D, Zhao L, Yang S, Liu J, Huang A, et al: Detection of circulating tumor cells and circulating tumor microemboli in gastric cancer. Transl Oncol. 10:431–441. 2017. View Article : Google Scholar :

32 

Galanzha EI, Shashkov EV, Kelly T, Kim JW, Yang L and Zharov VP: In vivo magnetic enrichment and multiplex photoacoustic detection of circulating tumour cells. Nat Nanotechnol. 4:855–860. 2009. View Article : Google Scholar :

33 

Zhang H, Jia Z, Wu C, Zang L, Yang G, Chen Z and Tang B: In vivo capture of circulating tumor cells based on transfusion with a vein indwelling needle. ACS Appl Mater Interfaces. 7:20477–20484. 2015. View Article : Google Scholar

34 

Plaks V, Koopman CD and Werb Z: Cancer. Circulating tumor cells. Science. 341:1186–1188. 2013. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Xiang A, Xue M, Ren F, Wang L, Ye Z, Li D, Ji Q, Ji G and Lu Z: High‑throughput and continuous flow isolation of rare circulating tumor cells and clusters in gastric cancer from human whole blood samples using electromagnetic vibration‑based filtration. Oncol Rep 43: 1975-1985, 2020.
APA
Xiang, A., Xue, M., Ren, F., Wang, L., Ye, Z., Li, D. ... Lu, Z. (2020). High‑throughput and continuous flow isolation of rare circulating tumor cells and clusters in gastric cancer from human whole blood samples using electromagnetic vibration‑based filtration. Oncology Reports, 43, 1975-1985. https://doi.org/10.3892/or.2020.7567
MLA
Xiang, A., Xue, M., Ren, F., Wang, L., Ye, Z., Li, D., Ji, Q., Ji, G., Lu, Z."High‑throughput and continuous flow isolation of rare circulating tumor cells and clusters in gastric cancer from human whole blood samples using electromagnetic vibration‑based filtration". Oncology Reports 43.6 (2020): 1975-1985.
Chicago
Xiang, A., Xue, M., Ren, F., Wang, L., Ye, Z., Li, D., Ji, Q., Ji, G., Lu, Z."High‑throughput and continuous flow isolation of rare circulating tumor cells and clusters in gastric cancer from human whole blood samples using electromagnetic vibration‑based filtration". Oncology Reports 43, no. 6 (2020): 1975-1985. https://doi.org/10.3892/or.2020.7567
Copy and paste a formatted citation
x
Spandidos Publications style
Xiang A, Xue M, Ren F, Wang L, Ye Z, Li D, Ji Q, Ji G and Lu Z: High‑throughput and continuous flow isolation of rare circulating tumor cells and clusters in gastric cancer from human whole blood samples using electromagnetic vibration‑based filtration. Oncol Rep 43: 1975-1985, 2020.
APA
Xiang, A., Xue, M., Ren, F., Wang, L., Ye, Z., Li, D. ... Lu, Z. (2020). High‑throughput and continuous flow isolation of rare circulating tumor cells and clusters in gastric cancer from human whole blood samples using electromagnetic vibration‑based filtration. Oncology Reports, 43, 1975-1985. https://doi.org/10.3892/or.2020.7567
MLA
Xiang, A., Xue, M., Ren, F., Wang, L., Ye, Z., Li, D., Ji, Q., Ji, G., Lu, Z."High‑throughput and continuous flow isolation of rare circulating tumor cells and clusters in gastric cancer from human whole blood samples using electromagnetic vibration‑based filtration". Oncology Reports 43.6 (2020): 1975-1985.
Chicago
Xiang, A., Xue, M., Ren, F., Wang, L., Ye, Z., Li, D., Ji, Q., Ji, G., Lu, Z."High‑throughput and continuous flow isolation of rare circulating tumor cells and clusters in gastric cancer from human whole blood samples using electromagnetic vibration‑based filtration". Oncology Reports 43, no. 6 (2020): 1975-1985. https://doi.org/10.3892/or.2020.7567
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