Open Access

Heparanase potentiates the invasion and migration of pancreatic cancer cells via epithelial‑to‑mesenchymal transition through the Wnt/β‑catenin pathway

  • Authors:
    • Cheng Wang
    • Yajun Wei
    • Gang Wang
    • Yangming Zhou
    • Jingcheng Zhang
    • Kai Xu
  • View Affiliations

  • Published online on: June 10, 2020     https://doi.org/10.3892/or.2020.7641
  • Pages: 711-721
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Mounting evidence indicates that there exists an association between heparanase (HPSE) and several physiological and pathological mechanisms in humans. However, the dynamics of the mechanisms involved in the regulation of HPSE expression in pancreatic cancer (PC) remain unclear. The aim of the present study was to assess the levels of HPSE in PC tissues and cell lines by western blotting and reverse transcription‑quantitative PCR (RT‑qPCR) analysis. Wound healing and Transwell assays were conducted to examine the effects of HPSE on migration and invasion in sh‑NC and sh‑HPSE PC cell lines. In addition, tumor growth was assessed in a mouse xenograft model in vivo. The expression levels of epithelial‑to‑mesenchymal transition (EMT)‑related biomarkers and the involvement of the Wnt/β‑catenin pathway were assessed by analyzing the results of western blot and RT‑qPCR assays. The results indicated that the expression of HPSE was substantially higher in PC tissues and cell lines, whereas experimental knockdown of HPSE suppressed the rates of migration and invasion of PC cells. Western blotting was used to assess the expression of EMT biomarkers and determine the function of HPSE in EMT. Furthermore, our results indicated that downregulation of HPSE expression decreased the expression of Wnt/β‑catenin associated proteins. In conclusion, HPSE appears to be a good candidate as a molecular target for the treatment of PC based on the finding of the present study.

Related Articles

Journal Cover

August-2020
Volume 44 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
APA
Wang, C., Wei, Y., Wang, G., Zhou, Y., Zhang, J., & Xu, K. (2020). Heparanase potentiates the invasion and migration of pancreatic cancer cells via epithelial‑to‑mesenchymal transition through the Wnt/β‑catenin pathway. Oncology Reports, 44, 711-721. https://doi.org/10.3892/or.2020.7641
MLA
Wang, C., Wei, Y., Wang, G., Zhou, Y., Zhang, J., Xu, K."Heparanase potentiates the invasion and migration of pancreatic cancer cells via epithelial‑to‑mesenchymal transition through the Wnt/β‑catenin pathway". Oncology Reports 44.2 (2020): 711-721.
Chicago
Wang, C., Wei, Y., Wang, G., Zhou, Y., Zhang, J., Xu, K."Heparanase potentiates the invasion and migration of pancreatic cancer cells via epithelial‑to‑mesenchymal transition through the Wnt/β‑catenin pathway". Oncology Reports 44, no. 2 (2020): 711-721. https://doi.org/10.3892/or.2020.7641