Long noncoding RNA UCID sponges miR‑152‑3p to promote colorectal cancer cell migration and invasion via the Wnt/β‑catenin signaling pathway Retraction in /10.3892/or.2023.8529

Sun,Li‑Bin; Zhao,Shu‑Fen; Zhu,Jing‑Juan; Han,Yue; Shan,Ti‑Dong

doi:10.3892/or.2020.7670

Long noncoding RNA UCID sponges miR‑152‑3p to promote colorectal cancer cell migration and invasion via the Wnt/β‑catenin signaling pathway

Retraction in: /10.3892/or.2023.8529

Authors:
- Li‑Bin Sun
- Shu‑Fen Zhao
- Jing‑Juan Zhu
- Yue Han
- Ti‑Dong Shan
View Affiliations

Affiliations: Department of Oncology and The Key Laboratory of Cancer Molecular and Translational Research, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 262000, P.R. China, Department of Gastroenterology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 262000, P.R. China
Published online on: July 6, 2020 https://doi.org/10.3892/or.2020.7670
Pages: 1194-1205

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Research has shown that long noncoding RNAs (lncRNAs) play significant roles in colorectal cancer (CRC). However, the role of lnc‑UCID (lncRNA upregulating CDK6 by interacting with DHX9) in CRC remains largely unknown. In the present study, analyses revealed that lnc‑UCID was markedly upregulated in CRC compared with that in normal specimens. Functional experiments showed that the depletion of lnc‑UCID inhibited CRC cell invasion and migration significantly, while overexpression of lnc‑UCID had the opposite effect. A candidate target of lnc‑UCID, microRNA miR‑152‑3p, was identified using bioinformatic analysis. Moreover, in CRC tissue, we noted an inverse correlation between miR‑152‑3p and lnc‑UCID expression levels. Overexpression and knockdown experiments revealed opposing roles for miR‑152‑3p and lnc‑UCID, suggesting that lnc‑UCID negatively regulates miR‑152‑3p. Luciferase reporter assays demonstrated that miR‑152‑3p directly targets lnc‑UCID. The results suggest that lnc‑UCID acts as an endogenous miRNA sponge, competing for miR‑152‑3p binding and thereby regulating the miRNA's targets. Overall, we propose that the lnc‑UCID/miR‑152‑3p/Wnt/β‑catenin signaling axis represents a novel mechanism that explains the migration and invasion of CRC.

View Figures

View References

1	Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar : PubMed/NCBI
2	Karsa LV, Lignini TA, Patnick J, Lambert R and Sauvaget C: The dimensions of the CRC problem. Best Pract Res Clin Gastroenterol. 24:381–396. 2010. View Article : Google Scholar : PubMed/NCBI
3	Brody H: Colorectal cancer. Nature. 521:S12015. View Article : Google Scholar : View Article : Google Scholar : PubMed/NCBI
4	Lichtenstern CR, Ngu RK, Shalapour S and Karin M: Immunotherapy, inflammation and colorectal cancer. Cells. 9:E6182020. View Article : Google Scholar : PubMed/NCBI
5	Lee J, Jeon JY and Meyerhardt JA: Diet and lifestyle in survivors of colorectal cancer. Hematol Oncol Clin North Am. 29:1–27. 2015. View Article : Google Scholar : PubMed/NCBI
6	Jäger T, Ocker M, Kiesslich T, Neureiter E and Neureiter D: Thoughts on investigational hedgehog pathway inhibitors for the treatment of cancer. Expert Opin Investig Drugs. 26:133–136. 2017. View Article : Google Scholar : PubMed/NCBI
7	Tay Y, Rinn J and Pandolfi PP: The multilayered complexity of ceRNA crosstalk and competition. Nature. 505:344–352. 2014. View Article : Google Scholar : PubMed/NCBI
8	Peng WX, Koirala P and Mo YY: LncRNA-mediated regulation of cell signaling in cancer. Oncogene. 36:5661–5667. 2017. View Article : Google Scholar : PubMed/NCBI
9	Forrest ME and Khalil AM: Review: Regulation of the cancer epigenome by long non-coding RNAs. Cancer Lett. 407:106–112. 2017. View Article : Google Scholar : PubMed/NCBI
10	Kopp F and Mendell JT: Functional classification and experimental dissection of long noncoding RNAs. Cell. 172:393–407. 2018. View Article : Google Scholar : PubMed/NCBI
11	Nam JW, Rissland OS, Koppstein D, Abreu-Goodger C, Jan CH, Agarwal V, Yildirim MA, Rodriguez A and Bartel DP: Global analyses of the effect of different cellular contexts on microRNA targeting. Mol Cell. 53:1031–1043. 2014. View Article : Google Scholar : PubMed/NCBI
12	Da Sacco L and Masotti A: Recent insights and novel bioinformatics tools to understand the role of microRNAs binding to 5′ untranslated region. Int J Mol Sci. 14:480–495. 2012. View Article : Google Scholar : PubMed/NCBI
13	Cesana M, Cacchiarelli D, Legnini I, Santini T, Sthandier O, Chinappi M, Tramontano A and Bozzoni I: A long noncoding RNA controls muscle differentiation by functioning as a competing endogenous RNA. Cell. 147:358–369. 2011. View Article : Google Scholar : PubMed/NCBI
14	Wang YL, Liu JY, Yang JE, Yu XM, Chen ZL, Chen YJ, Kuang M, Zhu Y and Zhuang SM: Lnc-UCID Promotes G1/S transition and hepatoma growth by preventing DHX9-mediated CDK6 downregulation. Hepatology. 70:259–275. 2019.PubMed/NCBI
15	Sobin LH, Gospodarowicz MK and Wittekind C: TNM classification of malignant tumors, 7th edition. Wiley-Blackwell. (Oxford). 2010.
16	Obrocea FL, Sajin M, Marinescu EC and Stoica D: Colorectal cancer and the 7th revision of the TNM staging system: Review of changes and suggestions for uniform pathologic reporting. Rom J Morphol Embryol. 52:537–544. 2011.PubMed/NCBI
17	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
18	Feng M, Zhang T and Ma H: Progesterone ameliorates the endometrial polyp by modulating the signaling pathway of Wnt and β-catenin via regulating the expression of H19 and miR-152. J Cell Biochem. 120:10164–10174. 2019. View Article : Google Scholar : PubMed/NCBI
19	Lv SY, Shan TD, Pan XT, Tian ZB, Liu XS, Liu FG, Sun XG, Xue HG, Li XH, Han Y, et al: The lncRNA ZEB1-AS1 sponges miR-181a-5p to promote colorectal cancer cell proliferation by regulating Wnt/β-catenin signaling. Cell Cycle. 17:1245–1254. 2018. View Article : Google Scholar : PubMed/NCBI
20	Vidovic D, Huynh TT, Konda P, Dean C, Cruickshank BM, Sultan M, Coyle KM, Gujar S and Marcato P: ALDH1A3-regulated long non-coding RNA NRAD1 is a potential novel target for triple-negative breast tumors and cancer stem cells. Cell Death Differ. 27:363–378. 2020. View Article : Google Scholar : PubMed/NCBI
21	Shan TD, Xu JH, Yu T, Li JY, Zhao LN, Ouyang H, Luo S, Lu XJ, Huang CZ, Lan QS, et al: Knockdown of linc-POU3F3 suppresses the proliferation, apoptosis, and migration resistance of colorectal cancer. Oncotarget. 7:961–975. 2016. View Article : Google Scholar : PubMed/NCBI
22	Kim MY: Long non-coding RNAs in cancer. Noncoding RNA Res. 4:452019. View Article : Google Scholar : PubMed/NCBI
23	Luo J, Wang K, Yeh S, Sun Y, Liang L, Xiao Y, Xu W, Niu Y, Cheng L, Maity SN, et al: LncRNA-p21 alters the antiandrogen enzalutamide-induced prostate cancer neuroendocrine differentiation via modulating the EZH2/STAT3 signaling. Nat Commun. 10:25712019. View Article : Google Scholar : PubMed/NCBI
24	Yu T, Shan TD, Li JY, Huang CZ, Wang SY, Ouyang H, Lu XJ, Xu JH, Zhong W and Chen QK: Knockdown of linc-UFC1 suppresses proliferation and induces apoptosis of colorectal cancer. Cell Death Dis. 7:e22282016. View Article : Google Scholar : PubMed/NCBI
25	Kołat D, Hammouz R, Bednarek AK and Płuciennik E: Exosomes as carriers transporting long noncoding RNAs: Molecular characteristics and their function in cancer (Review). Mol Med Rep. 20:851–862. 2019.PubMed/NCBI
26	Gharib E, Anaraki F, Baghdar K, Ghavidel P, Sadeghi H, Nasrabadi PN, Peyravian N, Aghdaei HA, Zali MR and Mojarad EN: Investigating the diagnostic performance of HOTTIP, PVT1, and UCA1 long noncoding RNAs as a predictive panel for the screening of colorectal cancer patients with lymph node metastasis. J Cell Biochem. 120:14780–14790. 2019. View Article : Google Scholar : PubMed/NCBI
27	Yu M, Bardia A, Wittner BS, Stott SL, Smas ME, Ting DT, Isakoff SJ, Ciciliano JC, Wells MN, Shah AM, et al: Circulating breast tumor cells exhibit dynamic changes in epithelial and mesenchymal composition. Science. 339:580–584. 2013. View Article : Google Scholar : PubMed/NCBI
28	Tam WL and Weinberg RA: The epigenetics of epithelial-mesenchymal plasticity in cancer. Nat Med. 19:1438–1449. 2013. View Article : Google Scholar : PubMed/NCBI
29	Wang M, Zhao F, Li S, Chang AK, Jia Z, Chen Y, Xu F, Pan H and Wu H: AIB1 cooperates with ERα to promote epithelial mesenchymal transition in breast cancer through SNAI1 activation. PLoS One. 8:e655562013. View Article : Google Scholar : PubMed/NCBI
30	Kolenda T, Guglas K, Kopczyńska M, Teresiak A, Bliźniak R, Mackiewicz A, Lamperska K and Mackiewicz J: Oncogenic role of ZFAS1 lncRNA in head and Neck squamous cell carcinomas. Cells. 8:3662019. View Article : Google Scholar
31	Conte F, Fiscon G, Chiara M, Colombo T, Farina L and Paci P: Role of the long non-coding RNA PVT1 in the dysregulation of the ceRNA-ceRNA network in human breast cancer. PLoS One. 12:e01716612017. View Article : Google Scholar : PubMed/NCBI
32	Song JL, Nigam P, Tektas SS and Selva E: microRNA regulation of Wnt signaling pathways in development and disease. Cell Signal. 27:1380–1391. 2015. View Article : Google Scholar : PubMed/NCBI
33	Sharma P, Saraya A and Sharma R: Serum-based six-miRNA signature as a potential marker for EC diagnosis: Comparison with TCGA miRNAseq dataset and identification of miRNA-mRNA target pairs by integrated analysis of TCGA miRNAseq and RNAseq datasets. Asia Pac J Clin Oncol. 14:e289–e301. 2018. View Article : Google Scholar : PubMed/NCBI