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MicroRNA‑16‑5p regulates cell survival, cell cycle and apoptosis by targeting AKT3 in prostate cancer cells

  • Authors:
    • Fang Wang
    • Wendi Wang
    • Lina Lu
    • Yi Xie
    • Junfang Yan
    • Yuhong Chen
    • Cuixia Di
    • Lu Gan
    • Jing Si
    • Hong Zhang
    • Aihong Mao

  • View Affiliations

  • Published online on: July 9, 2020     https://doi.org/10.3892/or.2020.7682
  • Pages: 1282-1292
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Abstract

Prostate cancer (PCa) is a malignancy with the highest morbidity rate in 105 countries worldwide and was a major cause of cancer‑associated death in men in 2018. Accumulating evidence suggests that microRNAs (miRNAs/miRs) have important functions in the carcinogenesis of PCa, and may provide novel treatment targets. Previous studies have indicated that miR‑16‑5p is associated with PCa. However, the relevance and importance of miR‑16‑5p in PCa carcinogenesis are still not completely understood. In the current study, we aimed to investigate the role and mechanism of miR‑16‑5p in PCa carcinogenesis. The results showed that miR‑16‑5p was markedly downregulated in PCa cells, and MTS assay, colony formation, flow cytometric analyses demonstrated that miR‑16‑5p inhibited PCa cell survival, regulated cell cycle distribution and induced apoptosis. Moreover, luciferase reporter assay and western blot analysis showed that miR‑16‑5p directly targets AKT3 (AKT serine/threonine kinase 3), which is associated with PCa carcinogenesis, and the effects of the downregulation of AKT3 were similar to the effects of upregulation of miR‑16‑5p in PC‑3 cells. In conclusion, our data clarify that miR‑16‑5p has anticancer functions in PCa cells, and our findings provide experimental evidence to highlight the potential value of miR‑targeting treatment strategies for PCa.
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September-2020
Volume 44 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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  • Impact Factor: 4.2
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  • CiteScore: 8.4
  • CiteScore Rank: Q1: #68/366 Oncology
  • Source Normalized Impact per Paper (SNIP): 0.786
  • SCImago Journal Rank (SJR): 0.812
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Spandidos Publications style
Wang F, Wang W, Lu L, Xie Y, Yan J, Chen Y, Di C, Gan L, Si J, Zhang H, Zhang H, et al: MicroRNA‑16‑5p regulates cell survival, cell cycle and apoptosis by targeting AKT3 in prostate cancer cells. Oncol Rep 44: 1282-1292, 2020
APA
Wang, F., Wang, W., Lu, L., Xie, Y., Yan, J., Chen, Y. ... Mao, A. (2020). MicroRNA‑16‑5p regulates cell survival, cell cycle and apoptosis by targeting AKT3 in prostate cancer cells. Oncology Reports, 44, 1282-1292. https://doi.org/10.3892/or.2020.7682
MLA
Wang, F., Wang, W., Lu, L., Xie, Y., Yan, J., Chen, Y., Di, C., Gan, L., Si, J., Zhang, H., Mao, A."MicroRNA‑16‑5p regulates cell survival, cell cycle and apoptosis by targeting AKT3 in prostate cancer cells". Oncology Reports 44.3 (2020): 1282-1292.
Chicago
Wang, F., Wang, W., Lu, L., Xie, Y., Yan, J., Chen, Y., Di, C., Gan, L., Si, J., Zhang, H., Mao, A."MicroRNA‑16‑5p regulates cell survival, cell cycle and apoptosis by targeting AKT3 in prostate cancer cells". Oncology Reports 44, no. 3 (2020): 1282-1292. https://doi.org/10.3892/or.2020.7682