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Article Open Access

SUMOylation of HSP27 regulates PKM2 to promote esophageal squamous cell carcinoma progression

  • Authors:
    • Xiao Zhang
    • Tao Liu
    • Shutao Zheng
    • Qing Liu
    • Tongxue Shen
    • Xiujuan Han
    • Qiqi Zhang
    • Lifei Yang
    • Xiaomei Lu
  • View Affiliations / Copyright

    Affiliations: Clinical Medical Research Institute, First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang Uygur Autonomous Region 830000, P.R. China, Health Management Center, Xinjiang Medical University, Urumqi, Xinjiang Uygur Autonomous Region 830000, P.R. China, Cancer Hospital Affiliated of Xinjiang Medical University, Urumqi, Xinjiang Uygur Autonomous Region 830000, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1355-1364
    |
    Published online on: July 31, 2020
       https://doi.org/10.3892/or.2020.7711
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Abstract

A previous proteomic screening of differentially expressed biomarkers between Kazakh patients with esophageal squamous cell carcinoma (ESCC) and normal adjacent tissues demonstrated that heat shock protein 27 (HSP27) and pyruvate kinase isoenzyme M2 (PKM2) were both highly expressed in ESCC samples compared with normal controls. However, the regulatory association between HSP27 and PKM2 in ESCC remains elusive. In the present study, immunohistochemistry and immunoblotting were adopted to examine the expression of HSP27, PKM2 and other relevant biomarkers involved in epithelial‑to‑mesenchymal transition in clinical tissue samples. The interactions between proteins were detected by co‑immunoprecipitation (Co‑IP) assay and further confirmed by immunofluorescence assay. The growth and motility of ESCC cells were examined by MTT, Transwell and wound healing assays. Overexpression of HSP27 was found to be significantly associated with T‑cell classification, lymph node metastasis and poor prognosis in ESCC. In addition, HSP27 expression was significantly correlated with PKM2 expression in ESCC specimens. Functionally, knockdown of HSP27 inhibited the growth and motility of ESCC cells. Moreover, HSP27 was found to directly interact with small ubiquitin‑related modified protein 2/3 (SUMO2/3) in ESCC cell lines, as evidenced by Co‑IP and laser confocal imaging. In addition, downregulation of HSP27 was shown to decrease PKM2 and E‑cadherin expression. Knockdown of SUMO2/3 was observed to reduce the expression of HSP27, PKM2 and EMT‑related biomarkers. The results of the present study indicated that the SUMOylation of HSP27 enhances the proliferation, invasion and migration of ESCC cells via PKM2.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang X, Liu T, Zheng S, Liu Q, Shen T, Han X, Zhang Q, Yang L and Lu X: SUMOylation of HSP27 regulates PKM2 to promote esophageal squamous cell carcinoma progression. Oncol Rep 44: 1355-1364, 2020.
APA
Zhang, X., Liu, T., Zheng, S., Liu, Q., Shen, T., Han, X. ... Lu, X. (2020). SUMOylation of HSP27 regulates PKM2 to promote esophageal squamous cell carcinoma progression. Oncology Reports, 44, 1355-1364. https://doi.org/10.3892/or.2020.7711
MLA
Zhang, X., Liu, T., Zheng, S., Liu, Q., Shen, T., Han, X., Zhang, Q., Yang, L., Lu, X."SUMOylation of HSP27 regulates PKM2 to promote esophageal squamous cell carcinoma progression". Oncology Reports 44.4 (2020): 1355-1364.
Chicago
Zhang, X., Liu, T., Zheng, S., Liu, Q., Shen, T., Han, X., Zhang, Q., Yang, L., Lu, X."SUMOylation of HSP27 regulates PKM2 to promote esophageal squamous cell carcinoma progression". Oncology Reports 44, no. 4 (2020): 1355-1364. https://doi.org/10.3892/or.2020.7711
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang X, Liu T, Zheng S, Liu Q, Shen T, Han X, Zhang Q, Yang L and Lu X: SUMOylation of HSP27 regulates PKM2 to promote esophageal squamous cell carcinoma progression. Oncol Rep 44: 1355-1364, 2020.
APA
Zhang, X., Liu, T., Zheng, S., Liu, Q., Shen, T., Han, X. ... Lu, X. (2020). SUMOylation of HSP27 regulates PKM2 to promote esophageal squamous cell carcinoma progression. Oncology Reports, 44, 1355-1364. https://doi.org/10.3892/or.2020.7711
MLA
Zhang, X., Liu, T., Zheng, S., Liu, Q., Shen, T., Han, X., Zhang, Q., Yang, L., Lu, X."SUMOylation of HSP27 regulates PKM2 to promote esophageal squamous cell carcinoma progression". Oncology Reports 44.4 (2020): 1355-1364.
Chicago
Zhang, X., Liu, T., Zheng, S., Liu, Q., Shen, T., Han, X., Zhang, Q., Yang, L., Lu, X."SUMOylation of HSP27 regulates PKM2 to promote esophageal squamous cell carcinoma progression". Oncology Reports 44, no. 4 (2020): 1355-1364. https://doi.org/10.3892/or.2020.7711
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