Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1021-335X Online ISSN: 1791-2431
Journal Cover
October-2020 Volume 44 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
October-2020 Volume 44 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML

  • Supplementary Files
    • Supplementary_Data.pdf
Article Open Access

MicroRNA‑188‑5p inhibits the progression of breast cancer by targeting zinc finger protein 91

  • Authors:
    • Zhiguang Yang
    • Zhaoyu Liu
    • Zhihui Chang
    • Guangxin Yan
  • View Affiliations / Copyright

    Affiliations: Department of Radiology, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1479-1488
    |
    Published online on: August 12, 2020
       https://doi.org/10.3892/or.2020.7731
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Breast cancer (BC) is the most commonly diagnosed malignant cancer in women. BC is the main cause of cancer‑related death in women and seriously threatens the life and health of women worldwide. MicroRNAs (miRNAs/miRs) have been reported to regulate the development and progression of different types of cancer. However, the regulatory functions of miR‑188‑5p in BC have not been thoroughly demonstrated. In this present research, we identified that miR‑188‑5p was downregulated in BC tissues and several BC cell lines. Downregulation of miR‑188‑5p was significantly associated with advanced TNM stage. Moreover, we identified that miR‑188‑5p mimics significantly inhibited proliferation using CCK‑8 assay, colony formation and xenograft animal model, suppressed invasion and migration detected by Transwell invasion assay, and increased the cellular apoptosis of BC cells as determined by cell apoptosis assay. Moreover miR‑188‑5p mimics also reduced the expression of NF‑κB p65(Rel). To further investigate its regulatory mechanism, transcription factor zinc finger protein 91 (ZFP91) was predicted as the targeted protein of miR‑188‑5p by bioinformatic method. We confirmed their specific binding by dual luciferase (DLR) assay. We demonstrated that the overexpression of miR‑188‑5p significantly inhibited the expression of ZFP91 in BC cell lines and reduced the expression of NF‑κB p65(Rel). An inverse correlation was found between the expression of miR‑188‑5p and ZFP91 in BC tissues. Importantly, we demonstrated that the restoration of ZFP91 was able to block the effect of miR‑188‑5p on the progression of MDA‑MB‑231 cells. Therefore, our study showed that miR‑188‑5p may be one of the important indicators and could inhibit the progression of human BC via targeting the ZFP91/NF‑κB p65(Rel) signaling pathway, suggesting that miR‑188‑5p may be a promising future target for BC treatment.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Davidson NE, Armstrong SA, Coussens LM, Cruz-Correa MR, DeBerardinis RJ, Doroshow JH, Foti M, Hwu P, Kensler TW, Morrow M, et al: AACR cancer progress report 2016. Clin Cancer Res. 22 (Suppl 19):S1–S137. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Ueno NT, Fernandez JRE, Cristofanilli M, Overmoyer B, Rea D, Berdichevski F, El-Shinawi M, Bellon J, Le-Petross HT, Lucci A, et al: International consensus on the clinical management of inflammatory breast cancer from the morgan welch inflammatory breast cancer research program 10th anniversary conference. J Cancer. 9:1437–1447. 2018. View Article : Google Scholar : PubMed/NCBI

3 

Lim B, Woodward WA, Wang X, Reuben JM and Ueno NT: Inflammatory breast cancer biology: The tumour microenvironment is key. Nat Rev Cancer. 18:485–499. 2018. View Article : Google Scholar : PubMed/NCBI

4 

Patnaik A, Rosen LS, Tolaney SM, Tolcher AW, Goldman JW, Gandhi L, Papadopoulos KP, Beeram M, Rasco DW, Hilton JF, et al: Efficacy and safety of abemaciclib, an inhibitor of CDK4 and CDK6, for patients with breast cancer, non-small cell lung cancer, and other solid tumors. Cancer Discov. 6:740–753. 2016. View Article : Google Scholar : PubMed/NCBI

5 

Bartel DP: MicroRNAs: Genomics, biogenesis, mechanism, and function. Cell. 116:281–297. 2004. View Article : Google Scholar : PubMed/NCBI

6 

Filipowicz W, Bhattacharyya SN and Sonenberg N: Mechanisms of post-transcriptional regulation by microRNAs: Are the answers in sight? Nat Rev Genet. 9:102–114. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Zhang H, Qi S, Zhang T, Wang A, Liu R, Guo J, Wang Y and Xu Y: miR-188-5p inhibits tumour growth and metastasis in prostate cancer by repressing LAPTM4B expression. Oncotarget. 6:6092–6104. 2015. View Article : Google Scholar : PubMed/NCBI

8 

Fang F, Chang RM, Yu L, Lei X, Xiao S, Yang H and Yang LY: MicroRNA-188-5p suppresses tumor cell proliferation and metastasis by directly targeting FGF5 in hepatocellular carcinoma. J Hepatol. 63:874–885. 2015. View Article : Google Scholar : PubMed/NCBI

9 

Edge SB, Byrd DR, Compton CC, Fritz AG, Greene FL and Trotti A: AJCC cancer staging manual. 7. Springer; New York, NY: 2010, PubMed/NCBI

10 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

11 

Jacob A, Jing J, Lee J, Schedin P, Gilbert SM, Peden AA, Junutula JR and Prekeris R: Rab40b regulates MMP2 and MMP9 trafficking during invadopodia formation and breast cancer cell invasion. J Cell Sci. 126:4647–4658. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Ma J, Mi C, Wang KS, Lee JJ and Jin X: Zinc finger protein 91 (ZFP91) activates HIF-1α via NF-κB/p65 to promote proliferation and tumorigenesis of colon cancer. Oncotarget. 7:365512016. View Article : Google Scholar : PubMed/NCBI

13 

Paschke L, Jopek K, Szyszka M, Tyczewska M, Ziolkowska A, Rucinski M and Malendowicz LK: ZFP91: A noncanonical NF-κB signaling pathway regulator with oncogenic properties is overexpressed in prostate cancer. Biomed Res Int. 2016:69635822016. View Article : Google Scholar : PubMed/NCBI

14 

Jin X, Jin HR, Jung HS, Lee SJ, Lee JH and Lee JJ: An atypical E3 ligase zinc finger protein 91 stabilizes and activates NF-kappaB-inducing kinase via Lys63-linked ubiquitination. J Biol Chem. 285:30539–30547. 2010. View Article : Google Scholar : PubMed/NCBI

15 

McGuire A, Brown JAL, Malone C, McLaughlin R and Kerin MJ: Effects of age on the detection and management of breast cancer. Cancers (Basel). 7:908–929. 2015. View Article : Google Scholar : PubMed/NCBI

16 

McLaughlin SA, Staley AC, Vicini F, Thiruchelvam P, Hutchison NA, Mendez J, MacNeill F, Rockson SG, DeSnyder SM, Klimberg S, et al: Considerations for clinicians in the diagnosis, prevention, and treatment of breast cancer-related lymphedema: Recommendations from a multidisciplinary expert ASBrS panel: Part 1: Definitions, assessments, education, and future directions. Ann Surg Oncol. 24:2818–2826. 2017. View Article : Google Scholar : PubMed/NCBI

17 

Romano G, Veneziano D, Acunzo M and Croce CM: Small non-coding RNA and cancer. Carcinogenesis. 38:485–491. 2017. View Article : Google Scholar : PubMed/NCBI

18 

Wang M, Qiu R, Gong Z, Zhao X, Wang T, Zhou L, Lu W, Shen B, Zhu W and Xu W: miR-188-5p emerges as an oncomiRNA to promote gastric cancer cell proliferation and migration via upregulation of SALL4. J Cell Biochem. 120:15027–15037. 2019. View Article : Google Scholar : PubMed/NCBI

19 

Li Y, Yan X, Shi J, He Y, Xu J, Lin L, Chen W and Lin X and Lin X: Aberrantly expressed miR-188-5p promotes gastric cancer metastasis by activating Wnt/β-catenin signaling. BMC Cancer. 19:5052019. View Article : Google Scholar : PubMed/NCBI

20 

Iwakawa HO and Tomari Y: The functions of microRNAs: mRNA decay and translational repression. Trends Cell Biol. 25:651–665. 2015. View Article : Google Scholar : PubMed/NCBI

21 

Wang M, Zhang H, Yang F, Qiu R, Zhao X, Gong Z, Yu W, Zhou B, Shen B and Zhu W: miR-188-5p suppresses cellular proliferation and migration via IL6ST: A potential noninvasive diagnostic biomarker for breast cancer. J Cell Physiol. 235:4890–4901. 2020. View Article : Google Scholar : PubMed/NCBI

22 

Saotome Y, Winter CG and Hirsh D: A widely expressed novel C2H2 zinc-finger protein with multiple consensus phosphorylation sites is conserved in mouse and man. Gene. 152:233–238. 1995. View Article : Google Scholar : PubMed/NCBI

23 

Lee JJ, Lee JH, Lee K, Hong YS and Jin XJ: Therapeutic agent for cancer, inflammation, and auto-immune disease containing inhibitor of zinc finger protein 91. US Patent 20080248024A1. Filed February 28, 2008; issued October 9, 2008.

24 

Paschke L, Jopek K, Szyszka M, Tyczewska M, Malendowicz LK and Rucinski M: ZFP91 zinc finger protein expression pattern in normal tissues and cancers. Oncol Lett. 17:3599–3606. 2019.PubMed/NCBI

25 

Unoki M, Okutsu J and Nakamura Y: Identification of a novel human gene, ZFP91, involved in acute myelogenous leukemia. Int J Oncol. 22:1217–1223. 2003.PubMed/NCBI

26 

Xiao G and Fu J: NF-κB and cancer: A paradigm of Yin-Yang. Am J Cancer Res. 1:192–221. 2011.PubMed/NCBI

27 

Tompkins V, Hagen J, Zediak VP and Quelle DE: Identification of novel ARF binding proteins by two-hybrid screening. Cell Cycle. 5:641–646. 2006. View Article : Google Scholar : PubMed/NCBI

28 

Peng Y, Shen X, Jiang H, Chen Z, Wu J, Zhu Y, Zhou Y and Li J: miR-188-5p suppresses gastric cancer cell proliferation and invasion via targeting ZFP91. Oncol Res. 27:65–71. 2018. View Article : Google Scholar : PubMed/NCBI

29 

Behm-Ansmant I, Rehwinkel J and Izaurralde E: MicroRNAs silence gene expression by repressing protein expression and/or by promoting mRNA decay. Cold Spring Harb Symp Quant Biol. 71:523–530. 2006. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Yang Z, Liu Z, Chang Z and Yan G: MicroRNA‑188‑5p inhibits the progression of breast cancer by targeting zinc finger protein 91. Oncol Rep 44: 1479-1488, 2020.
APA
Yang, Z., Liu, Z., Chang, Z., & Yan, G. (2020). MicroRNA‑188‑5p inhibits the progression of breast cancer by targeting zinc finger protein 91. Oncology Reports, 44, 1479-1488. https://doi.org/10.3892/or.2020.7731
MLA
Yang, Z., Liu, Z., Chang, Z., Yan, G."MicroRNA‑188‑5p inhibits the progression of breast cancer by targeting zinc finger protein 91". Oncology Reports 44.4 (2020): 1479-1488.
Chicago
Yang, Z., Liu, Z., Chang, Z., Yan, G."MicroRNA‑188‑5p inhibits the progression of breast cancer by targeting zinc finger protein 91". Oncology Reports 44, no. 4 (2020): 1479-1488. https://doi.org/10.3892/or.2020.7731
Copy and paste a formatted citation
x
Spandidos Publications style
Yang Z, Liu Z, Chang Z and Yan G: MicroRNA‑188‑5p inhibits the progression of breast cancer by targeting zinc finger protein 91. Oncol Rep 44: 1479-1488, 2020.
APA
Yang, Z., Liu, Z., Chang, Z., & Yan, G. (2020). MicroRNA‑188‑5p inhibits the progression of breast cancer by targeting zinc finger protein 91. Oncology Reports, 44, 1479-1488. https://doi.org/10.3892/or.2020.7731
MLA
Yang, Z., Liu, Z., Chang, Z., Yan, G."MicroRNA‑188‑5p inhibits the progression of breast cancer by targeting zinc finger protein 91". Oncology Reports 44.4 (2020): 1479-1488.
Chicago
Yang, Z., Liu, Z., Chang, Z., Yan, G."MicroRNA‑188‑5p inhibits the progression of breast cancer by targeting zinc finger protein 91". Oncology Reports 44, no. 4 (2020): 1479-1488. https://doi.org/10.3892/or.2020.7731
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team