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Circular RNA NOX4 promotes the development of colorectal cancer via the microRNA‑485‑5p/CKS1B axis

Retraction in: /10.3892/or.2024.8780
  • Authors:
    • Ximin Wang
    • Geng Tao
    • Donghong Huang
    • Shuangyin Liang
    • Dongxu Zheng
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian 362000, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2009-2020
    |
    Published online on: September 7, 2020
       https://doi.org/10.3892/or.2020.7758
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Abstract

Colorectal cancer (CRC) is a common malignancy globally. The aim of the present study was to explore the role and the working mechanism of circular RNA NADPH oxidase 4 (circNOX4; circBase ID, hsa_circ_0023990) in CRC. Reverse transcription‑quantitative (RT‑q)PCR was used to examine the expression of circNOX4, NOX4 mRNA and microRNA (miR)‑485‑5p in CRC tissues and cell lines. 3‑(4,5‑Dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide and Transwell assays were performed to analyze CRC cell viability and motility. The glycolytic ability of CRC cells was assessed by measuring glucose consumption, lactate production, extracellular acidification and O2 consumption rates using commercial kits. The starBase database was used to predict the targets of circNOX4 and miR‑485‑5p, and the interaction was confirmed by dual‑luciferase reporter and RNA immunoprecipitation assays. A murine xenograft model was established to verify the role of circNOX4 in CRC in vivo. The results demonstrated that the expression of circNOX4 was upregulated in CRC tissues and cell lines compared with that in adjacent normal tissues and a normal colon epithelial cell line, respectively. The expression of circNOX4 was negatively associated with the prognosis of patients with CRC. CircNOX4 silencing suppressed the proliferation, migration, invasion and glycolysis of CRC cells. miR‑485‑5p was identified as a target of circNOX4. CircNOX4 promoted CRC progression by sponging miR‑485‑5p. miR‑485‑5p was demonstrated to bind to the 3' untranslated region (UTR) of CDC28 protein kinase regulatory subunit 1B (CKS1B). miR‑485‑5p overexpression‑mediated malignant properties of CRC cells were partly reversed by the transfection with the CKS1B overexpression plasmid. CircNOX4 silencing restrained the CRC xenograft growth in vivo. Collectively, the results of the present study demonstrated that circNOX4 may serve an oncogenic role in CRC by promoting the proliferation, migration, invasion and glycolysis of CRC cells via the miR‑485‑5p/CKS1B axis.
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Copy and paste a formatted citation
Spandidos Publications style
Wang X, Tao G, Huang D, Liang S and Zheng D: Circular RNA NOX4 promotes the development of colorectal cancer via the microRNA‑485‑5p/CKS1B axis Retraction in /10.3892/or.2024.8780. Oncol Rep 44: 2009-2020, 2020.
APA
Wang, X., Tao, G., Huang, D., Liang, S., & Zheng, D. (2020). Circular RNA NOX4 promotes the development of colorectal cancer via the microRNA‑485‑5p/CKS1B axis Retraction in /10.3892/or.2024.8780. Oncology Reports, 44, 2009-2020. https://doi.org/10.3892/or.2020.7758
MLA
Wang, X., Tao, G., Huang, D., Liang, S., Zheng, D."Circular RNA NOX4 promotes the development of colorectal cancer via the microRNA‑485‑5p/CKS1B axis Retraction in /10.3892/or.2024.8780". Oncology Reports 44.5 (2020): 2009-2020.
Chicago
Wang, X., Tao, G., Huang, D., Liang, S., Zheng, D."Circular RNA NOX4 promotes the development of colorectal cancer via the microRNA‑485‑5p/CKS1B axis Retraction in /10.3892/or.2024.8780". Oncology Reports 44, no. 5 (2020): 2009-2020. https://doi.org/10.3892/or.2020.7758
Copy and paste a formatted citation
x
Spandidos Publications style
Wang X, Tao G, Huang D, Liang S and Zheng D: Circular RNA NOX4 promotes the development of colorectal cancer via the microRNA‑485‑5p/CKS1B axis Retraction in /10.3892/or.2024.8780. Oncol Rep 44: 2009-2020, 2020.
APA
Wang, X., Tao, G., Huang, D., Liang, S., & Zheng, D. (2020). Circular RNA NOX4 promotes the development of colorectal cancer via the microRNA‑485‑5p/CKS1B axis Retraction in /10.3892/or.2024.8780. Oncology Reports, 44, 2009-2020. https://doi.org/10.3892/or.2020.7758
MLA
Wang, X., Tao, G., Huang, D., Liang, S., Zheng, D."Circular RNA NOX4 promotes the development of colorectal cancer via the microRNA‑485‑5p/CKS1B axis Retraction in /10.3892/or.2024.8780". Oncology Reports 44.5 (2020): 2009-2020.
Chicago
Wang, X., Tao, G., Huang, D., Liang, S., Zheng, D."Circular RNA NOX4 promotes the development of colorectal cancer via the microRNA‑485‑5p/CKS1B axis Retraction in /10.3892/or.2024.8780". Oncology Reports 44, no. 5 (2020): 2009-2020. https://doi.org/10.3892/or.2020.7758
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