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Article

Tannic acid attenuates hepatic oxidative stress, apoptosis and inflammation by activating the Keap1‑Nrf2/ARE signaling pathway in arsenic trioxide‑toxicated rats

  • Authors:
    • Mengying Li
    • Panpan Liu
    • Yucong Xue
    • Yingran Liang
    • Jing Shi
    • Xue Han
    • Jianping Zhang
    • Xi Chu
    • Li Chu
  • View Affiliations / Copyright

    Affiliations: School of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050200, P.R. China, Department of Pharmacy, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China, School of Basic Medicine, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050200, P.R. China
  • Pages: 2306-2316
    |
    Published online on: September 11, 2020
       https://doi.org/10.3892/or.2020.7764
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Abstract

The present study was performed to investigate the protective effects of tannic acid (TA) on liver injury induced by arsenic trioxide (ATO) and to elucidate the mechanism involved as related to the Kelch‑like ECH‑associated protein 1 (Keap1)‑nuclear factor erythroid 2‑related factor 2 (Nrf2)/antioxidant response element (ARE) signaling pathway. Adult rats were intraperitoneally injected with TA, while ATO was administered 1 h later. On the 11th day, the rats were euthanized to determine any liver histological changes, liver function, and the activities of antioxidant, antiapoptosis and proinflammatory cytokines in the liver. Furthermore, the protein expression levels of nuclear Nrf2, total Nrf2, Keap1, Heme oxygenase‑1 (HO‑1), NADPH quinine oxidoreductase‑1 (NQO1), and γ‑glutamylcysteine synthetase (γ‑GCS) were determined using western blot analysis. The results showed that TA treatment ameliorated ATO‑induced liver histological changes and decreased the ATO‑induced increased alanine aminotransferase (ALT) and aspartate transaminase (AST) serum levels. Activities of the antioxidant enzymes significantly were increased, while the levels of malondialdehyde (MDA) and reactive oxygen species (ROS) were attenuated following TA treatment. In addition, TA treatment inhibited ATO‑induced liver apoptosis and inflammatory responses, increased Bcl‑2 protein expression level and reduced the levels of Bax, caspase‑3, interleukin (IL)‑1β, IL‑6 and tumor necrosis factor (TNF)‑α. Furthermore, TA treatment increased the protein expression levels of Nrf2 and Keap1, HO‑1, NQO1 and γ‑GCS. The results demonstrated that TA has a protective effect on ATO‑treated hepatic toxicity and that its underlying mechanism could be due to TA activation of the Keap1‑Nrf2/ARE signaling pathway, to reduce oxidative stress, apoptosis and inflammation in ATO‑intoxicated rats.
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Copy and paste a formatted citation
Spandidos Publications style
Li M, Liu P, Xue Y, Liang Y, Shi J, Han X, Zhang J, Chu X and Chu L: Tannic acid attenuates hepatic oxidative stress, apoptosis and inflammation by activating the Keap1‑Nrf2/ARE signaling pathway in arsenic trioxide‑toxicated rats. Oncol Rep 44: 2306-2316, 2020.
APA
Li, M., Liu, P., Xue, Y., Liang, Y., Shi, J., Han, X. ... Chu, L. (2020). Tannic acid attenuates hepatic oxidative stress, apoptosis and inflammation by activating the Keap1‑Nrf2/ARE signaling pathway in arsenic trioxide‑toxicated rats. Oncology Reports, 44, 2306-2316. https://doi.org/10.3892/or.2020.7764
MLA
Li, M., Liu, P., Xue, Y., Liang, Y., Shi, J., Han, X., Zhang, J., Chu, X., Chu, L."Tannic acid attenuates hepatic oxidative stress, apoptosis and inflammation by activating the Keap1‑Nrf2/ARE signaling pathway in arsenic trioxide‑toxicated rats". Oncology Reports 44.5 (2020): 2306-2316.
Chicago
Li, M., Liu, P., Xue, Y., Liang, Y., Shi, J., Han, X., Zhang, J., Chu, X., Chu, L."Tannic acid attenuates hepatic oxidative stress, apoptosis and inflammation by activating the Keap1‑Nrf2/ARE signaling pathway in arsenic trioxide‑toxicated rats". Oncology Reports 44, no. 5 (2020): 2306-2316. https://doi.org/10.3892/or.2020.7764
Copy and paste a formatted citation
x
Spandidos Publications style
Li M, Liu P, Xue Y, Liang Y, Shi J, Han X, Zhang J, Chu X and Chu L: Tannic acid attenuates hepatic oxidative stress, apoptosis and inflammation by activating the Keap1‑Nrf2/ARE signaling pathway in arsenic trioxide‑toxicated rats. Oncol Rep 44: 2306-2316, 2020.
APA
Li, M., Liu, P., Xue, Y., Liang, Y., Shi, J., Han, X. ... Chu, L. (2020). Tannic acid attenuates hepatic oxidative stress, apoptosis and inflammation by activating the Keap1‑Nrf2/ARE signaling pathway in arsenic trioxide‑toxicated rats. Oncology Reports, 44, 2306-2316. https://doi.org/10.3892/or.2020.7764
MLA
Li, M., Liu, P., Xue, Y., Liang, Y., Shi, J., Han, X., Zhang, J., Chu, X., Chu, L."Tannic acid attenuates hepatic oxidative stress, apoptosis and inflammation by activating the Keap1‑Nrf2/ARE signaling pathway in arsenic trioxide‑toxicated rats". Oncology Reports 44.5 (2020): 2306-2316.
Chicago
Li, M., Liu, P., Xue, Y., Liang, Y., Shi, J., Han, X., Zhang, J., Chu, X., Chu, L."Tannic acid attenuates hepatic oxidative stress, apoptosis and inflammation by activating the Keap1‑Nrf2/ARE signaling pathway in arsenic trioxide‑toxicated rats". Oncology Reports 44, no. 5 (2020): 2306-2316. https://doi.org/10.3892/or.2020.7764
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