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SPAG6 promotes cell proliferation and inhibits apoptosis through the PTEN/PI3K/AKT pathway in Burkitt lymphoma

  • Authors:
    • Rongrong Zhang
    • Haiyan Zhu
    • Yufang Yuan
    • Yun Wang
    • Zhaofang Tian
  • View Affiliations / Copyright

    Affiliations: Department of Pediatrics, The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University, Huaian, Jiangsu 223300, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2021-2030
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    Published online on: September 22, 2020
       https://doi.org/10.3892/or.2020.7776
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Abstract

The main purpose of the present study was to elucidate the role of sperm‑associated antigen 6 (SPAG6) in the occurrence and development of Burkitt lymphoma (BL) and explore the underlying molecular mechanisms. A correlation was observed between the expression of SPAG6 and the prognosis of patients with lymphoma using The Cancer Genome Atlas (TCGA) database analysis. It was demonstrated that the levels of SPAG6 in BL cells were higher compared with that in IM‑9 cells by reverse transcription‑PCR and western blot assays. Moreover, silencing of SPAG6 significantly decreased proliferation and increased apoptosis of Daudi and Raji cells, whereas SPAG6 overexpression exerted the opposite effects on CA46 and NAMALWA cells. When investigating the possible mechanism, it was first observed that the level of phosphatase and tensin homolog (PTEN) protein was significantly increased, while that of phosphorylated (p‑)AKT protein was markedly reduced in the SPAG6‑knockdown group compared with the blank control group in Daudi and Raji cells by western blot analysis. It was further ascertained whether the phosphoinositide 3‑kinase (PI3K)/PTEN/protein kinase B (AKT) pathway mediates the effects of SPAG6 on cell proliferation and apoptosis, and the results demonstrated that silencing of SPAG6 suppressed the viability of Daudi and Raji cells, whereas PTEN knockdown using siRNA or SF1670 (a specific PTEN inhibitor) reversed the inhibitory effect on cell proliferation and the promoting effect on cell apoptosis induced by SPAG6 depletion in vitro as well as in vivo. These data revealed that SPAG6 may promote the proliferation and inhibit the apoptosis of BL cells via the PTEN/PI3K/AKT pathway. The results of the present study suggest that SPAG6 may play a key role in the progression of BL and may be of value as a predictive prognostic biomarker in patients with BL.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang R, Zhu H, Yuan Y, Wang Y and Tian Z: SPAG6 promotes cell proliferation and inhibits apoptosis through the PTEN/PI3K/AKT pathway in Burkitt lymphoma. Oncol Rep 44: 2021-2030, 2020.
APA
Zhang, R., Zhu, H., Yuan, Y., Wang, Y., & Tian, Z. (2020). SPAG6 promotes cell proliferation and inhibits apoptosis through the PTEN/PI3K/AKT pathway in Burkitt lymphoma. Oncology Reports, 44, 2021-2030. https://doi.org/10.3892/or.2020.7776
MLA
Zhang, R., Zhu, H., Yuan, Y., Wang, Y., Tian, Z."SPAG6 promotes cell proliferation and inhibits apoptosis through the PTEN/PI3K/AKT pathway in Burkitt lymphoma". Oncology Reports 44.5 (2020): 2021-2030.
Chicago
Zhang, R., Zhu, H., Yuan, Y., Wang, Y., Tian, Z."SPAG6 promotes cell proliferation and inhibits apoptosis through the PTEN/PI3K/AKT pathway in Burkitt lymphoma". Oncology Reports 44, no. 5 (2020): 2021-2030. https://doi.org/10.3892/or.2020.7776
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang R, Zhu H, Yuan Y, Wang Y and Tian Z: SPAG6 promotes cell proliferation and inhibits apoptosis through the PTEN/PI3K/AKT pathway in Burkitt lymphoma. Oncol Rep 44: 2021-2030, 2020.
APA
Zhang, R., Zhu, H., Yuan, Y., Wang, Y., & Tian, Z. (2020). SPAG6 promotes cell proliferation and inhibits apoptosis through the PTEN/PI3K/AKT pathway in Burkitt lymphoma. Oncology Reports, 44, 2021-2030. https://doi.org/10.3892/or.2020.7776
MLA
Zhang, R., Zhu, H., Yuan, Y., Wang, Y., Tian, Z."SPAG6 promotes cell proliferation and inhibits apoptosis through the PTEN/PI3K/AKT pathway in Burkitt lymphoma". Oncology Reports 44.5 (2020): 2021-2030.
Chicago
Zhang, R., Zhu, H., Yuan, Y., Wang, Y., Tian, Z."SPAG6 promotes cell proliferation and inhibits apoptosis through the PTEN/PI3K/AKT pathway in Burkitt lymphoma". Oncology Reports 44, no. 5 (2020): 2021-2030. https://doi.org/10.3892/or.2020.7776
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