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EHF enhances malignancy by modulating AKT and MAPK/ERK signaling in non‑small cell lung cancer cells

  • Authors:
    • Lei Gao
    • Tian Yang
    • Shuo Zhang
    • Yiqian Liang
    • Puyu Shi
    • Hui Ren
    • Peng Hou
    • Mingwei Chen
  • View Affiliations / Copyright

    Affiliations: Department of Endocrinology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China, Shanxi Provincial Research Center for The Project of Prevention and Treatment of Respiratory Diseases, Xi'an, Shaanxi 710061, P.R. China, Department of Endocrinology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China
    Copyright: © Gao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 102
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    Published online on: April 15, 2021
       https://doi.org/10.3892/or.2021.8053
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Abstract

Overexpression of ETS‑homologous factor (EHF) in non‑small cell lung cancer (NSCLC) is associated with poor patient prognosis. To explore the mechanism of the effect of EHF in NSCLC, EHF expression was examined in NSCLC and its role in cell proliferation, invasion, cell cycle, and apoptosis of NSCLC cells was evaluated by overexpressing EHF and/or knocking down EHF expression in NSCLC cells in vitro and in cancer cell grafted mice in vivo. The results revealed that the knockdown of EHF expression in NSCLC with siRNA significantly inhibited cell proliferation and invasion, arrested the cell cycle at the G0/G1 phase, and induced apoptosis, whereas overexpression of EHF in NSCLC promoted cell proliferation, tumor growth, and cancer cell migration in vitro. The in vivo experiments demonstrated that siRNA‑mediated downregulation of EHF expression in NSCLC cells significantly suppressed tumor growth in xenografted nude mice as compared to cancer progression in the mice grafted with NSCLC cells transfected with non‑specific control siRNA. The biochemical analyses revealed that EHF promoted NSCLC growth by regulating the transcription of Erb‑B2 receptor tyrosine kinase 2/3 (ERBB2, ERBB3) and mesenchymal‑epithelial transition (MET) factor tyrosine kinase receptors and modulating the AKT and ERK signaling pathways in the NSCLC cells. The present findings indicated that EHF could be used as a prognostic marker for NSCLC, and tyrosine kinase receptors of ERBB2, ERBB3 and MET could be drug targets for NSCLC treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Gao L, Yang T, Zhang S, Liang Y, Shi P, Ren H, Hou P and Chen M: EHF enhances malignancy by modulating AKT and MAPK/ERK signaling in non‑small cell lung cancer cells. Oncol Rep 45: 102, 2021.
APA
Gao, L., Yang, T., Zhang, S., Liang, Y., Shi, P., Ren, H. ... Chen, M. (2021). EHF enhances malignancy by modulating AKT and MAPK/ERK signaling in non‑small cell lung cancer cells. Oncology Reports, 45, 102. https://doi.org/10.3892/or.2021.8053
MLA
Gao, L., Yang, T., Zhang, S., Liang, Y., Shi, P., Ren, H., Hou, P., Chen, M."EHF enhances malignancy by modulating AKT and MAPK/ERK signaling in non‑small cell lung cancer cells". Oncology Reports 45.6 (2021): 102.
Chicago
Gao, L., Yang, T., Zhang, S., Liang, Y., Shi, P., Ren, H., Hou, P., Chen, M."EHF enhances malignancy by modulating AKT and MAPK/ERK signaling in non‑small cell lung cancer cells". Oncology Reports 45, no. 6 (2021): 102. https://doi.org/10.3892/or.2021.8053
Copy and paste a formatted citation
x
Spandidos Publications style
Gao L, Yang T, Zhang S, Liang Y, Shi P, Ren H, Hou P and Chen M: EHF enhances malignancy by modulating AKT and MAPK/ERK signaling in non‑small cell lung cancer cells. Oncol Rep 45: 102, 2021.
APA
Gao, L., Yang, T., Zhang, S., Liang, Y., Shi, P., Ren, H. ... Chen, M. (2021). EHF enhances malignancy by modulating AKT and MAPK/ERK signaling in non‑small cell lung cancer cells. Oncology Reports, 45, 102. https://doi.org/10.3892/or.2021.8053
MLA
Gao, L., Yang, T., Zhang, S., Liang, Y., Shi, P., Ren, H., Hou, P., Chen, M."EHF enhances malignancy by modulating AKT and MAPK/ERK signaling in non‑small cell lung cancer cells". Oncology Reports 45.6 (2021): 102.
Chicago
Gao, L., Yang, T., Zhang, S., Liang, Y., Shi, P., Ren, H., Hou, P., Chen, M."EHF enhances malignancy by modulating AKT and MAPK/ERK signaling in non‑small cell lung cancer cells". Oncology Reports 45, no. 6 (2021): 102. https://doi.org/10.3892/or.2021.8053
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