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Role of SIRT1/AMPK signaling in the proliferation, migration and invasion of renal cell carcinoma cells

  • Authors:
    • Xin Wang
    • Youlu Lu
    • Zhouting Tuo
    • Huan Zhou
    • Ying Zhang
    • Zhangjun Cao
    • Longfei Peng
    • Dexin Yu
    • Liangkuan Bi
  • View Affiliations / Copyright

    Affiliations: Department of Urology, The Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230032, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 109
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    Published online on: April 19, 2021
       https://doi.org/10.3892/or.2021.8060
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Abstract

Renal cell carcinoma (RCC) is a lethal urologic tumor commonly seen in men that best responds to partial nephrectomy. An enhanced understanding of the molecular pathogenesis of RCC can broaden treatment options and tumor prevention strategies. Sirtuin 1 (SIRT1) is a NAD+‑dependent deacetylase that regulates several bioactive substances, and the present study aimed to identify the role of SIRT1/AMP‑activated protein kinase (AMPK) signaling in RCC progression. SIRT1 expression was detected in 100 patients with RCC using tissue microarray immunohistochemistry. SIRT1‑knockdown and overexpression were performed via RNA interference and plasmid transfection. Inhibition of AMPK was used for the phenotypic rescue assays to verify whether AMPK was a downstream target of SIRT1. Reverse transcription‑quantitative PCR was performed to verify transfection efficiency. Transwell, MTT and flow cytometry apoptosis assays were performed to evaluate the migration, invasion, proliferation and early apoptosis level of RCC cells. SIRT1 and AMPK protein expression in human RCC tissues and cell lines (786‑O and ACHN) was detected using western blotting and immunofluorescence staining. The current results, combined with data from The Cancer Genome Atlas database, revealed that SIRT1 expression in RCC tissues was downregulated compared with in adjacent normal tissues. Additionally, high SIRT1 expression was associated with an improved prognosis in patients with RCC. Overexpression of SIRT1 inhibited the proliferation, migration and invasion of RCC cell lines and induced apoptosis, while inhibition of SIRT1 expression had the opposite effects. Further experiments indicated that SIRT1 may serve an anticancer role by upregulating the expression levels of downstream AMPK, thus revealing a potential therapeutic target for RCC.
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Copy and paste a formatted citation
Spandidos Publications style
Wang X, Lu Y, Tuo Z, Zhou H, Zhang Y, Cao Z, Peng L, Yu D and Bi L: Role of SIRT1/AMPK signaling in the proliferation, migration and invasion of renal cell carcinoma cells. Oncol Rep 45: 109, 2021.
APA
Wang, X., Lu, Y., Tuo, Z., Zhou, H., Zhang, Y., Cao, Z. ... Bi, L. (2021). Role of SIRT1/AMPK signaling in the proliferation, migration and invasion of renal cell carcinoma cells. Oncology Reports, 45, 109. https://doi.org/10.3892/or.2021.8060
MLA
Wang, X., Lu, Y., Tuo, Z., Zhou, H., Zhang, Y., Cao, Z., Peng, L., Yu, D., Bi, L."Role of SIRT1/AMPK signaling in the proliferation, migration and invasion of renal cell carcinoma cells". Oncology Reports 45.6 (2021): 109.
Chicago
Wang, X., Lu, Y., Tuo, Z., Zhou, H., Zhang, Y., Cao, Z., Peng, L., Yu, D., Bi, L."Role of SIRT1/AMPK signaling in the proliferation, migration and invasion of renal cell carcinoma cells". Oncology Reports 45, no. 6 (2021): 109. https://doi.org/10.3892/or.2021.8060
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Spandidos Publications style
Wang X, Lu Y, Tuo Z, Zhou H, Zhang Y, Cao Z, Peng L, Yu D and Bi L: Role of SIRT1/AMPK signaling in the proliferation, migration and invasion of renal cell carcinoma cells. Oncol Rep 45: 109, 2021.
APA
Wang, X., Lu, Y., Tuo, Z., Zhou, H., Zhang, Y., Cao, Z. ... Bi, L. (2021). Role of SIRT1/AMPK signaling in the proliferation, migration and invasion of renal cell carcinoma cells. Oncology Reports, 45, 109. https://doi.org/10.3892/or.2021.8060
MLA
Wang, X., Lu, Y., Tuo, Z., Zhou, H., Zhang, Y., Cao, Z., Peng, L., Yu, D., Bi, L."Role of SIRT1/AMPK signaling in the proliferation, migration and invasion of renal cell carcinoma cells". Oncology Reports 45.6 (2021): 109.
Chicago
Wang, X., Lu, Y., Tuo, Z., Zhou, H., Zhang, Y., Cao, Z., Peng, L., Yu, D., Bi, L."Role of SIRT1/AMPK signaling in the proliferation, migration and invasion of renal cell carcinoma cells". Oncology Reports 45, no. 6 (2021): 109. https://doi.org/10.3892/or.2021.8060
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