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AMOTL2‑knockdown promotes the proliferation, migration and invasion of glioma by regulating β‑catenin nuclear localization

  • Authors:
    • Xingjie Chen
    • Yalin Lu
    • Gaochao Guo
    • Yu Zhang
    • Yan Sun
    • Lianmei Guo
    • Ruohong Li
    • Yang Nan
    • Xuejun Yang
    • Jun Dong
    • Xun Jin
    • Qiang Huang
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China, Department of Neurosurgery, Henan Provincial People's Hospital, Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450003, P.R. China, Department of Neurosurgery, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong 264000, P.R. China, Department of Neurosurgery, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 139
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    Published online on: May 25, 2021
       https://doi.org/10.3892/or.2021.8090
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Abstract

Glioblastoma multiforme (GBM) is the most prevalent type of malignant cancer in the adult central nervous system; however, its mechanism remains unclear. Angiomotin‑like 2 (AMOTL2) is a member of the motin family of angiostatin‑binding proteins. It has been reported as an oncogene in cervical and breast cancer, but its association with glioma remains unknown. The aim of the present study was to investigate AMOTL2‑regulated processes in glioma cell lines using extensive in vitro assays and certain bioinformatics tools. These results revealed that AMOTL2 was downregulated in high‑grade glioma cells and tissues, with patients with glioma exhibiting a high AMOTL2 expression having a higher survival rate. The results of the glioma cell phenotype experiment showed that AMOTL2 suppressed GBM proliferation, migration and invasion. In addition, immunoblotting, co‑immunoprecipitation and immunofluorescence assays demonstrated that AMOTL2 could directly bind to β‑catenin protein, the key molecule of the Wnt signaling pathway, and regulate its downstream genes by regulating β‑catenin nuclear translocation. In conclusion, the present study demonstrated that AMOTL2 inhibited glioma proliferation, migration and invasion by regulating β‑catenin nuclear localization. Thus, AMOTL2 may serve as a therapeutic target to further improve the prognosis and prolong survival time of patients with glioma.
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Copy and paste a formatted citation
Spandidos Publications style
Chen X, Lu Y, Guo G, Zhang Y, Sun Y, Guo L, Li R, Nan Y, Yang X, Dong J, Dong J, et al: AMOTL2‑knockdown promotes the proliferation, migration and invasion of glioma by regulating β‑catenin nuclear localization. Oncol Rep 46: 139, 2021.
APA
Chen, X., Lu, Y., Guo, G., Zhang, Y., Sun, Y., Guo, L. ... Huang, Q. (2021). AMOTL2‑knockdown promotes the proliferation, migration and invasion of glioma by regulating β‑catenin nuclear localization. Oncology Reports, 46, 139. https://doi.org/10.3892/or.2021.8090
MLA
Chen, X., Lu, Y., Guo, G., Zhang, Y., Sun, Y., Guo, L., Li, R., Nan, Y., Yang, X., Dong, J., Jin, X., Huang, Q."AMOTL2‑knockdown promotes the proliferation, migration and invasion of glioma by regulating β‑catenin nuclear localization". Oncology Reports 46.1 (2021): 139.
Chicago
Chen, X., Lu, Y., Guo, G., Zhang, Y., Sun, Y., Guo, L., Li, R., Nan, Y., Yang, X., Dong, J., Jin, X., Huang, Q."AMOTL2‑knockdown promotes the proliferation, migration and invasion of glioma by regulating β‑catenin nuclear localization". Oncology Reports 46, no. 1 (2021): 139. https://doi.org/10.3892/or.2021.8090
Copy and paste a formatted citation
x
Spandidos Publications style
Chen X, Lu Y, Guo G, Zhang Y, Sun Y, Guo L, Li R, Nan Y, Yang X, Dong J, Dong J, et al: AMOTL2‑knockdown promotes the proliferation, migration and invasion of glioma by regulating β‑catenin nuclear localization. Oncol Rep 46: 139, 2021.
APA
Chen, X., Lu, Y., Guo, G., Zhang, Y., Sun, Y., Guo, L. ... Huang, Q. (2021). AMOTL2‑knockdown promotes the proliferation, migration and invasion of glioma by regulating β‑catenin nuclear localization. Oncology Reports, 46, 139. https://doi.org/10.3892/or.2021.8090
MLA
Chen, X., Lu, Y., Guo, G., Zhang, Y., Sun, Y., Guo, L., Li, R., Nan, Y., Yang, X., Dong, J., Jin, X., Huang, Q."AMOTL2‑knockdown promotes the proliferation, migration and invasion of glioma by regulating β‑catenin nuclear localization". Oncology Reports 46.1 (2021): 139.
Chicago
Chen, X., Lu, Y., Guo, G., Zhang, Y., Sun, Y., Guo, L., Li, R., Nan, Y., Yang, X., Dong, J., Jin, X., Huang, Q."AMOTL2‑knockdown promotes the proliferation, migration and invasion of glioma by regulating β‑catenin nuclear localization". Oncology Reports 46, no. 1 (2021): 139. https://doi.org/10.3892/or.2021.8090
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