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Article

Diosmin in combination with naringenin enhances apoptosis in colon cancer cells

  • Authors:
    • Bushra Zeya
    • Sana Nafees
    • Khalid Imtiyaz
    • Laraib Uroog
    • Khalid Umar Fakhri
    • M. Moshahid A. Rizvi
  • View Affiliations / Copyright

    Affiliations: The Genome Biology Laboratory, Department of Biosciences, Ramanujan Block, Jamia Millia Islamia, Jamia Nagar, New Delhi, Delhi 110025, India
  • Article Number: 4
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    Published online on: November 2, 2021
       https://doi.org/10.3892/or.2021.8215
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Abstract

Colon cancer is one of the most commonly diagnosed malignancies, which begins as a polyp and grows to become cancer. Diosmin (DS) and naringenin (NR) are naturally occurring flavonoids that exhibit various pharmacological activities. Although several studies have illustrated the effectiveness of these flavonoids as anti‑cancerous agents individually, the combinatorial impact of these compounds has not been explored. In the present study, the combined effect of DS and NR (DiNar) in colon cancer cell lines HCT116 and SW480 were assessed by targeting apoptosis and inflammatory pathways. The MTT assay was used to evaluate the effect of DiNar on cell proliferation, while Chou‑Talalay analysis was employed to determine the combination index of DS and NR. Moreover, flow cytometry was used to monitor cell cycle arrest and population study. The onset of apoptosis was assessed by DAPI staining, DNA fragmentation, and Annexin V‑fluorescein isothiocyanate/propidium iodide (Annexin V‑FITC/PI). The expression levels of apoptotic pathway markers, Bcl‑2, Bax, caspase3, caspase8, caspase9 and p53, and inflammatory markers, NF‑κβ, IKK‑α and IKK‑β, were assessed using western blotting and reverse transcription‑quantitative PCR. These results suggested that DiNar treatment acts synergistically and induces cytotoxicity with a concomitant increase in chromatin condensation, DNA fragmentation and cell cycle arrest in the G0/G1 phase. Annexin V‑FITC/PI apoptosis assay also showed increased number of cells undergoing apoptosis in the DiNar treatment group. Furthermore, the expression of apoptosis and inflammatory markers was also more effectively regulated under the DiNar treatment. Thereby, these findings demonstrated that DiNar treatment could be a potential novel chemotherapeutic alternative in colon cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Zeya B, Nafees S, Imtiyaz K, Uroog L, Fakhri KU and Rizvi MM: Diosmin in combination with naringenin enhances apoptosis in colon cancer cells. Oncol Rep 47: 4, 2022.
APA
Zeya, B., Nafees, S., Imtiyaz, K., Uroog, L., Fakhri, K.U., & Rizvi, M.M. (2022). Diosmin in combination with naringenin enhances apoptosis in colon cancer cells. Oncology Reports, 47, 4. https://doi.org/10.3892/or.2021.8215
MLA
Zeya, B., Nafees, S., Imtiyaz, K., Uroog, L., Fakhri, K. U., Rizvi, M. M."Diosmin in combination with naringenin enhances apoptosis in colon cancer cells". Oncology Reports 47.1 (2022): 4.
Chicago
Zeya, B., Nafees, S., Imtiyaz, K., Uroog, L., Fakhri, K. U., Rizvi, M. M."Diosmin in combination with naringenin enhances apoptosis in colon cancer cells". Oncology Reports 47, no. 1 (2022): 4. https://doi.org/10.3892/or.2021.8215
Copy and paste a formatted citation
x
Spandidos Publications style
Zeya B, Nafees S, Imtiyaz K, Uroog L, Fakhri KU and Rizvi MM: Diosmin in combination with naringenin enhances apoptosis in colon cancer cells. Oncol Rep 47: 4, 2022.
APA
Zeya, B., Nafees, S., Imtiyaz, K., Uroog, L., Fakhri, K.U., & Rizvi, M.M. (2022). Diosmin in combination with naringenin enhances apoptosis in colon cancer cells. Oncology Reports, 47, 4. https://doi.org/10.3892/or.2021.8215
MLA
Zeya, B., Nafees, S., Imtiyaz, K., Uroog, L., Fakhri, K. U., Rizvi, M. M."Diosmin in combination with naringenin enhances apoptosis in colon cancer cells". Oncology Reports 47.1 (2022): 4.
Chicago
Zeya, B., Nafees, S., Imtiyaz, K., Uroog, L., Fakhri, K. U., Rizvi, M. M."Diosmin in combination with naringenin enhances apoptosis in colon cancer cells". Oncology Reports 47, no. 1 (2022): 4. https://doi.org/10.3892/or.2021.8215
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