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Hypoxia induces radioresistance, epithelial‑mesenchymal transition, cancer stem cell‑like phenotype and changes in genes possessing multiple biological functions in head and neck squamous cell carcinoma

  • Authors:
    • Emilia Wiechec
    • Natasa Matic
    • Ashfaq Ali
    • Karin Roberg
  • View Affiliations / Copyright

    Affiliations: Department of Biomedical and Clinical Sciences, Division of Cell Biology, Linköping University, 58185 Linköping, Sweden, Department of Otorhinolaryngology in Linköping, Anaesthetics, Operations and Specialty Surgery Center, Region Östergötland, 58185 Linköping, Sweden, National Bioinformatics Infrastructure Sweden, Science for Life Laboratory; Department of Immune Technology, Lund University, 22100 Lund, Sweden
    Copyright: © Wiechec et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 58
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    Published online on: January 21, 2022
       https://doi.org/10.3892/or.2022.8269
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Abstract

Hypoxia has been linked with increased resistance to treatment in various solid tumors, including head and neck squamous cell carcinoma (HNSCC). The aim of the present study was to identify genes involved in hypoxia‑mediated responses to radiotherapy in HNSCC. A total of three HNSCC cell lines with an epithelial phenotype were selected for this study and cultured under normoxic (21% O2) or hypoxic (1% O2) conditions. The sensitivity of the HNSCC cells to radiotherapy was assessed by a crystal violet assay. Western blotting (for protein expression), cDNA microarrays and reverse transcription‑quantitative PCR (for gene expression) were also applied. Small interfering RNA silencing was used to knock down target genes. The results revealed that hypoxia negatively affected the response of HNSCC cells to radiotherapy. Of note, increased levels of N‑cadherin, vimentin and fibronectin, as well as stem cell‑associated transcription factors, were observed under hypoxia. The microarray analysis revealed a number of hypoxia‑regulated genes that were involved in multiple biological functions. However, downregulation of hypoxia‑regulated genes did not affect sensitivity to radiotherapy of the investigated cell lines. Taken together, the present findings indicated several important pathways and genes that were involved in hypoxia and radiotherapy resistance. It is hypothesized that panels of reported hypoxia‑regulated genes may be useful for the prediction of radiotherapy responses in patients with HNSCC.
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Copy and paste a formatted citation
Spandidos Publications style
Wiechec E, Matic N, Ali A and Roberg K: Hypoxia induces radioresistance, epithelial‑mesenchymal transition, cancer stem cell‑like phenotype and changes in genes possessing multiple biological functions in head and neck squamous cell carcinoma. Oncol Rep 47: 58, 2022.
APA
Wiechec, E., Matic, N., Ali, A., & Roberg, K. (2022). Hypoxia induces radioresistance, epithelial‑mesenchymal transition, cancer stem cell‑like phenotype and changes in genes possessing multiple biological functions in head and neck squamous cell carcinoma. Oncology Reports, 47, 58. https://doi.org/10.3892/or.2022.8269
MLA
Wiechec, E., Matic, N., Ali, A., Roberg, K."Hypoxia induces radioresistance, epithelial‑mesenchymal transition, cancer stem cell‑like phenotype and changes in genes possessing multiple biological functions in head and neck squamous cell carcinoma". Oncology Reports 47.3 (2022): 58.
Chicago
Wiechec, E., Matic, N., Ali, A., Roberg, K."Hypoxia induces radioresistance, epithelial‑mesenchymal transition, cancer stem cell‑like phenotype and changes in genes possessing multiple biological functions in head and neck squamous cell carcinoma". Oncology Reports 47, no. 3 (2022): 58. https://doi.org/10.3892/or.2022.8269
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Spandidos Publications style
Wiechec E, Matic N, Ali A and Roberg K: Hypoxia induces radioresistance, epithelial‑mesenchymal transition, cancer stem cell‑like phenotype and changes in genes possessing multiple biological functions in head and neck squamous cell carcinoma. Oncol Rep 47: 58, 2022.
APA
Wiechec, E., Matic, N., Ali, A., & Roberg, K. (2022). Hypoxia induces radioresistance, epithelial‑mesenchymal transition, cancer stem cell‑like phenotype and changes in genes possessing multiple biological functions in head and neck squamous cell carcinoma. Oncology Reports, 47, 58. https://doi.org/10.3892/or.2022.8269
MLA
Wiechec, E., Matic, N., Ali, A., Roberg, K."Hypoxia induces radioresistance, epithelial‑mesenchymal transition, cancer stem cell‑like phenotype and changes in genes possessing multiple biological functions in head and neck squamous cell carcinoma". Oncology Reports 47.3 (2022): 58.
Chicago
Wiechec, E., Matic, N., Ali, A., Roberg, K."Hypoxia induces radioresistance, epithelial‑mesenchymal transition, cancer stem cell‑like phenotype and changes in genes possessing multiple biological functions in head and neck squamous cell carcinoma". Oncology Reports 47, no. 3 (2022): 58. https://doi.org/10.3892/or.2022.8269
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