Oxymatrine suppresses oral squamous cell carcinoma progression by suppressing CXC chemokine receptor 4 in an m6A modification decrease dependent manner

  • Authors:
    • Renhui Luo
    • Lili Xie
    • Yingmei Lin
    • Jun Shao
    • Zhejing Lin
  • View Affiliations

  • Published online on: August 23, 2022     https://doi.org/10.3892/or.2022.8392
  • Article Number: 177
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Abstract

Oxymatrine has been revealed to exert antitumor activity; however, its role in oral squamous cell carcinoma (OSCC) remains unclear. In the present study, the effects and underlying molecular mechanisms of oxymatrine in OSCC were explored. The antineoplastic effects of oxymatrine were measured using Cell Counting Kit‑8, apoptosis and Transwell assays. The inhibitory effect of oxymatrine on tumor growth was evaluated in vivo. The regulation of oxymatrine on the CXC chemokine receptor 4 (CXCR4) was analyzed using western blotting, reverse transcription‑quantitative PCR, RNA stability and methylated RNA immunoprecipitation assays. The present results revealed that oxymatrine inhibited the proliferation and migration of OSCC cells and promoted cell apoptosis. Furthermore, oxymatrine reduced CXCR4 mRNA and protein expression levels by promoting CXCR4 mRNA degradation. Mechanistically, oxymatrine inhibited the methylation at the N6‑position of adenosine (m6A modification) of CXCR4 mRNA by decreasing the expression of the methyltransferase‑like 3 (METTL3) gene. In addition, oxymatrine inhibited tumor growth in vivo. Taken together, our findings demonstrated the antitumor effect of oxymatrine on OSCC. Mechanistically, oxymatrine inhibited the progression of OSCC by downregulating METTL3 and degrading CXCR4 mRNA by decreasing the level of m6A modification.
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October-2022
Volume 48 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Luo R, Xie L, Lin Y, Shao J and Lin Z: Oxymatrine suppresses oral squamous cell carcinoma progression by suppressing CXC chemokine receptor 4 in an m<sup>6</sup>A modification decrease dependent manner. Oncol Rep 48: 177, 2022
APA
Luo, R., Xie, L., Lin, Y., Shao, J., & Lin, Z. (2022). Oxymatrine suppresses oral squamous cell carcinoma progression by suppressing CXC chemokine receptor 4 in an m<sup>6</sup>A modification decrease dependent manner. Oncology Reports, 48, 177. https://doi.org/10.3892/or.2022.8392
MLA
Luo, R., Xie, L., Lin, Y., Shao, J., Lin, Z."Oxymatrine suppresses oral squamous cell carcinoma progression by suppressing CXC chemokine receptor 4 in an m<sup>6</sup>A modification decrease dependent manner". Oncology Reports 48.4 (2022): 177.
Chicago
Luo, R., Xie, L., Lin, Y., Shao, J., Lin, Z."Oxymatrine suppresses oral squamous cell carcinoma progression by suppressing CXC chemokine receptor 4 in an m<sup>6</sup>A modification decrease dependent manner". Oncology Reports 48, no. 4 (2022): 177. https://doi.org/10.3892/or.2022.8392