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Wnt2b and Wnt5a expression is highly associated with M2 TAMs in non‑small cell lung cancer

  • Authors:
    • Ryota Sumitomo
    • Cheng-Long Huang
    • Hidenori Ando
    • Tatsuhiro Ishida
    • Hiroyuki Cho
    • Hiroshi Date
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic Surgery, Tazuke Kofukai Medical Research Institute, Kitano Hospital, Osaka 530‑8480, Japan, Department of Pharmacokinetics and Biopharmaceutics, Institute of Biomedical Science, Tokushima University, Tokushima 770‑8505, Japan, Department of Thoracic Surgery, Graduate School of Medicine, Kyoto University, Kyoto 606‑8507, Japan
    Copyright: © Sumitomo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 189
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    Published online on: September 13, 2022
       https://doi.org/10.3892/or.2022.8404
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Abstract

Tumor‑associated macrophages (TAMs), particularly M2 macrophages, promote tumor progression, while Wnt genes encode a family of multi‑functional glycoproteins that serve an important role in tumorigenesis. Immunohistochemical studies were performed to evaluate Wnt2b and Wnt5a expression in tumor and stromal cells in M2 and M1 TAMs and Ki‑67 proliferation index in 160 consecutive patients with resected non‑small cell lung cancer (NSCLC). Overall, 52 tumors (32.5%) were classified as tumoral Wnt2b‑high (Wnt2b‑positive tumor cells >30%) and 95 (59.4%) as stromal Wnt2b‑high (Wnt2b‑positive stromal cells >30%), while 75 (46.9%) were classified as tumoral Wnt5a‑high (Wnt5a‑positive tumor cells >30%) and 63 (39.4%) as stromal Wnt5a‑high (Wnt5a‑positive stromal cells >28%). The density of M2 TAMs was significantly higher in the tumoral (P=0.0024) and stromal Wnt2b‑high groups (P=0.0054). The density of M2 TAMs was also significantly higher in the tumoral (P=0.0005) and stromal Wnt5a‑high groups (P=0.0486). By contrast, no difference in stromal or islet M1 TAM density was observed in relation to tumoral or stromal Wnt2b or Wnt5a status. Furthermore, Ki‑67 proliferation index was significantly higher in the tumoral (P=0.0121) and stromal Wnt2b‑high (P=0.0019) and tumoral Wnt5a‑high (P=0.0088) groups. Overall survival rate was significantly lower in the Wnt2b‑high (P=0.0437), Wnt5a‑high (P=0.0106) and M2 TAM‑high (P=0.0060) groups. Wnt2b and Wnt5a expression in tumor and stromal cells may induce M2 TAMs to produce more aggressive behavior during tumor progression in NSCLC.
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Copy and paste a formatted citation
Spandidos Publications style
Sumitomo R, Huang C, Ando H, Ishida T, Cho H and Date H: Wnt2b and Wnt5a expression is highly associated with M2 TAMs in non‑small cell lung cancer. Oncol Rep 48: 189, 2022.
APA
Sumitomo, R., Huang, C., Ando, H., Ishida, T., Cho, H., & Date, H. (2022). Wnt2b and Wnt5a expression is highly associated with M2 TAMs in non‑small cell lung cancer. Oncology Reports, 48, 189. https://doi.org/10.3892/or.2022.8404
MLA
Sumitomo, R., Huang, C., Ando, H., Ishida, T., Cho, H., Date, H."Wnt2b and Wnt5a expression is highly associated with M2 TAMs in non‑small cell lung cancer". Oncology Reports 48.5 (2022): 189.
Chicago
Sumitomo, R., Huang, C., Ando, H., Ishida, T., Cho, H., Date, H."Wnt2b and Wnt5a expression is highly associated with M2 TAMs in non‑small cell lung cancer". Oncology Reports 48, no. 5 (2022): 189. https://doi.org/10.3892/or.2022.8404
Copy and paste a formatted citation
x
Spandidos Publications style
Sumitomo R, Huang C, Ando H, Ishida T, Cho H and Date H: Wnt2b and Wnt5a expression is highly associated with M2 TAMs in non‑small cell lung cancer. Oncol Rep 48: 189, 2022.
APA
Sumitomo, R., Huang, C., Ando, H., Ishida, T., Cho, H., & Date, H. (2022). Wnt2b and Wnt5a expression is highly associated with M2 TAMs in non‑small cell lung cancer. Oncology Reports, 48, 189. https://doi.org/10.3892/or.2022.8404
MLA
Sumitomo, R., Huang, C., Ando, H., Ishida, T., Cho, H., Date, H."Wnt2b and Wnt5a expression is highly associated with M2 TAMs in non‑small cell lung cancer". Oncology Reports 48.5 (2022): 189.
Chicago
Sumitomo, R., Huang, C., Ando, H., Ishida, T., Cho, H., Date, H."Wnt2b and Wnt5a expression is highly associated with M2 TAMs in non‑small cell lung cancer". Oncology Reports 48, no. 5 (2022): 189. https://doi.org/10.3892/or.2022.8404
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