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HMGB1 promotes the development of castration‑resistant prostate cancer by regulating androgen receptor activation

  • Authors:
    • Junyu Chen
    • Duo Xu
    • Taiwei Wang
    • Zhaoyun Yang
    • Yanrong Yang
    • Kang He
    • Lijing Zhao
  • View Affiliations / Copyright

    Affiliations: Department of Rehabilitation, School of Nursing, Jilin University, Changchun, Jilin 130021, P.R. China, School of Medicine, Tongji University, Shanghai 200092, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 197
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    Published online on: September 19, 2022
       https://doi.org/10.3892/or.2022.8412
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Abstract

AR signalling pathway reactivation plays a key role in the development of castration‑resistant prostate cancer (CRPC). High‑mobility group protein B1 (HMGB1) is an important factor involved in the occurrence and development of a variety of tumours by regulating gene transcription. In the present study, the association between HMGB1 and prostate cancer (PCa) and the effects of HMGB1 on androgen receptor (AR) transcription and signalling pathway reactivation in PCa cells in vitro and in vivo were evaluated. A bioinformatics method was used to determine the mRNA expression level of HMGB1 in PCa specimens and its correlation with the mRNA expression of AR. Immunohistochemical staining was used to detect the expression of these proteins in clinical PCa samples. Reporter gene and ChIP assays were performed to determine the activity of AR and the effect of HMGB1 on the ability of AR to bind to the promoters of prostate specific antigen and transmembrane protease, serine 2. A bioluminescence resonance energy transfer assay was employed to observe the direct interaction between HMGB1 and AR protein. Additionally, a castrated nude mouse xenograft tumour model was established to verify the effect of HMGB1 on PCa. The results revealed that HMGB1 expression was significantly increased in PCa specimens, which may have a strong correlation with AR expression. Moreover, HMGB1 could reactivate the AR signalling pathway, directly interact with AR, and promote the development of CRPC in an androgen‑independent manner. The results of the present study indicated that HMGB1 promoted the development of CRPC by interacting with AR, which inferred that decreasing the expression of HMGB1 may be a potential effective method for CRPC prevention and treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Chen J, Xu D, Wang T, Yang Z, Yang Y, He K and Zhao L: HMGB1 promotes the development of castration‑resistant prostate cancer by regulating androgen receptor activation. Oncol Rep 48: 197, 2022.
APA
Chen, J., Xu, D., Wang, T., Yang, Z., Yang, Y., He, K., & Zhao, L. (2022). HMGB1 promotes the development of castration‑resistant prostate cancer by regulating androgen receptor activation. Oncology Reports, 48, 197. https://doi.org/10.3892/or.2022.8412
MLA
Chen, J., Xu, D., Wang, T., Yang, Z., Yang, Y., He, K., Zhao, L."HMGB1 promotes the development of castration‑resistant prostate cancer by regulating androgen receptor activation". Oncology Reports 48.5 (2022): 197.
Chicago
Chen, J., Xu, D., Wang, T., Yang, Z., Yang, Y., He, K., Zhao, L."HMGB1 promotes the development of castration‑resistant prostate cancer by regulating androgen receptor activation". Oncology Reports 48, no. 5 (2022): 197. https://doi.org/10.3892/or.2022.8412
Copy and paste a formatted citation
x
Spandidos Publications style
Chen J, Xu D, Wang T, Yang Z, Yang Y, He K and Zhao L: HMGB1 promotes the development of castration‑resistant prostate cancer by regulating androgen receptor activation. Oncol Rep 48: 197, 2022.
APA
Chen, J., Xu, D., Wang, T., Yang, Z., Yang, Y., He, K., & Zhao, L. (2022). HMGB1 promotes the development of castration‑resistant prostate cancer by regulating androgen receptor activation. Oncology Reports, 48, 197. https://doi.org/10.3892/or.2022.8412
MLA
Chen, J., Xu, D., Wang, T., Yang, Z., Yang, Y., He, K., Zhao, L."HMGB1 promotes the development of castration‑resistant prostate cancer by regulating androgen receptor activation". Oncology Reports 48.5 (2022): 197.
Chicago
Chen, J., Xu, D., Wang, T., Yang, Z., Yang, Y., He, K., Zhao, L."HMGB1 promotes the development of castration‑resistant prostate cancer by regulating androgen receptor activation". Oncology Reports 48, no. 5 (2022): 197. https://doi.org/10.3892/or.2022.8412
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