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Gomisin A enhances the antitumor effect of paclitaxel by suppressing oxidative stress in ovarian cancer

  • Authors:
    • Taiwei Wang
    • Jian Liu
    • Xuemiao Huang
    • Chuanqi Zhang
    • Mengyuan Shangguan
    • Junyu Chen
    • Shan Wu
    • Mengmeng Chen
    • Zhaoyun Yang
    • Shuhua Zhao
  • View Affiliations / Copyright

    Affiliations: Department of Gynaecology and Obstetrics, The Second Hospital, Jilin University, Changchun, Jilin 130021, P.R. China, Department of Rehabilitation, School of Nursing, Jilin University, Changchun, Jilin 130022, P.R. China, Department of Reproductive Endocrinology, Women's Hospital, Zhejiang University, School of Medicine, Hangzhou, Zhejiang 310006, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 202
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    Published online on: September 27, 2022
       https://doi.org/10.3892/or.2022.8417
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Abstract

Gomisin A (GA) is an effective component of Schisandra. The crude extracts of Schisandra chinensis and its active ingredients have been shown to inhibit multidrug resistance in tumour cells. Reactive oxygen species (ROS) have different roles in cancer and may contribute to therapy resistance. The human ovarian cancer (OC) cell lines SKOV3 and A2780, and a mouse model of OC, were used in the present study. MTT assay, colony formation assay, flow cytometry, western blot analysis, and haematoxylin and eosin (H&E) staining were performed to determine the antitumor effect of GA and paclitaxel (PTX) in vitro and in vivo. The ROS inhibitor N‑acetyl cysteine (NAC) was used to assess the mechanism underlying the chemosensitizing effects of GA. Notably, the proliferation of OC cells was inhibited by PTX, which could be enhanced by the ROS inhibitor NAC or GA. Treatment with NAC + PTX or GA + PTX enhanced the cell cycle arrest, but not apoptosis, induced by PTX. Moreover, the molecular mechanism underlying this effect may be that GA decreases the levels of ROS in ovarian cancer cells and inhibits cell cycle progression by downregulating the expression of the cell cycle proteins cyclin‑dependent kinase 4 and cyclin B1. In conclusion, the combination of PTX and the ROS inhibitor GA may be a novel strategy in OC chemotherapy.
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Copy and paste a formatted citation
Spandidos Publications style
Wang T, Liu J, Huang X, Zhang C, Shangguan M, Chen J, Wu S, Chen M, Yang Z, Zhao S, Zhao S, et al: Gomisin A enhances the antitumor effect of paclitaxel by suppressing oxidative stress in ovarian cancer. Oncol Rep 48: 202, 2022.
APA
Wang, T., Liu, J., Huang, X., Zhang, C., Shangguan, M., Chen, J. ... Zhao, S. (2022). Gomisin A enhances the antitumor effect of paclitaxel by suppressing oxidative stress in ovarian cancer. Oncology Reports, 48, 202. https://doi.org/10.3892/or.2022.8417
MLA
Wang, T., Liu, J., Huang, X., Zhang, C., Shangguan, M., Chen, J., Wu, S., Chen, M., Yang, Z., Zhao, S."Gomisin A enhances the antitumor effect of paclitaxel by suppressing oxidative stress in ovarian cancer". Oncology Reports 48.5 (2022): 202.
Chicago
Wang, T., Liu, J., Huang, X., Zhang, C., Shangguan, M., Chen, J., Wu, S., Chen, M., Yang, Z., Zhao, S."Gomisin A enhances the antitumor effect of paclitaxel by suppressing oxidative stress in ovarian cancer". Oncology Reports 48, no. 5 (2022): 202. https://doi.org/10.3892/or.2022.8417
Copy and paste a formatted citation
x
Spandidos Publications style
Wang T, Liu J, Huang X, Zhang C, Shangguan M, Chen J, Wu S, Chen M, Yang Z, Zhao S, Zhao S, et al: Gomisin A enhances the antitumor effect of paclitaxel by suppressing oxidative stress in ovarian cancer. Oncol Rep 48: 202, 2022.
APA
Wang, T., Liu, J., Huang, X., Zhang, C., Shangguan, M., Chen, J. ... Zhao, S. (2022). Gomisin A enhances the antitumor effect of paclitaxel by suppressing oxidative stress in ovarian cancer. Oncology Reports, 48, 202. https://doi.org/10.3892/or.2022.8417
MLA
Wang, T., Liu, J., Huang, X., Zhang, C., Shangguan, M., Chen, J., Wu, S., Chen, M., Yang, Z., Zhao, S."Gomisin A enhances the antitumor effect of paclitaxel by suppressing oxidative stress in ovarian cancer". Oncology Reports 48.5 (2022): 202.
Chicago
Wang, T., Liu, J., Huang, X., Zhang, C., Shangguan, M., Chen, J., Wu, S., Chen, M., Yang, Z., Zhao, S."Gomisin A enhances the antitumor effect of paclitaxel by suppressing oxidative stress in ovarian cancer". Oncology Reports 48, no. 5 (2022): 202. https://doi.org/10.3892/or.2022.8417
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