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DPP3 expression promotes cell proliferation and migration in vitro and tumour growth in vivo, which is associated with poor prognosis of oesophageal carcinoma

  • Authors:
    • Jing-Kun Liu
    • Abulizi Abudula
    • Hai-Tao Yang
    • Li-Xiu Xu
    • Yiliyaer Nuerrula
    • Ge Bai
    • Aisiker Tulahong
    • Maynur Eli
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, The First Affiliated Hospital of Xinjiang Medical University, State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Xinjiang Medical University, Urumqi, Xinjiang Uyghur Autonomous Region 830011, P.R. China, School of Medicine, Ningde Normal University, Ningde, Fujian 352000, P.R. China, Department of Cardiology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang Uyghur Autonomous Region 830011, P.R. China, Department of Pathology, College of Basic Medicine, Medical University of Xinjiang, Urumqi, Xinjiang Uyghur Autonomous Region 836001, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 9
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    Published online on: November 15, 2022
       https://doi.org/10.3892/or.2022.8446
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Abstract

Dipeptidyl peptidase III (DPP3), a zinc‑dependent metallopeptidase, is upregulated in a variety of malignancies. However, little is known about its roles in the pathogenesis of these malignancies. The present study was designed to investigate the roles of DPP3 in the pathogenesis and progression of oesophageal cancer (EC). The expression level of DPP3 in EC tissues and adjacent normal tissues was detected in 93 cases of tissue biopsies collected from patients diagnosed with oesophageal carcinoma by immunohistochemistry. The effect of DPP3 expression on cell proliferation, migration or apoptosis was determined in DPP3‑depleted EC cells created by infection with lentivirus containing short hairpin RNA specific to the human DPP3 mRNA sequence, followed by detection at the cellular level using a Celigo cell count assay, flow cytometry, wound‑healing assay and Transwell assay as well as chip screening with a Human Apoptosis Antibody Array kit, which enables the quantitative detection of 43 apoptosis‑related genes. A xenograft model was applied to detect the tumour growth and invasion of DPP3‑depleted cancer cells in nude mice. The results revealed that DPP3 expression was elevated in EC tissues compared with adjacent non‑tumour tissues, and high DPP3 expression was significantly associated with poor prognosis. DPP3 depletion resulted in reduced cell proliferation and migration and enhanced cell cycle arrest and apoptosis of EC cells and led to the inhibition of tumour growth and invasion in a xenograft model. In addition, DPP3 depletion was associated with the upregulation of the proapoptotic proteins SMAC and p53 and the downregulation of the antiapoptotic proteins clAP‑2, IGFBP‑2 and TRAILR‑4. Finally, DPP3 may promote cell proliferation, migration and survival of EC cells in vitro and tumour growth and invasion of oesophageal carcinoma in vivo, and thus may serve as a molecular target for tumour therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Liu J, Abudula A, Yang H, Xu L, Nuerrula Y, Bai G, Tulahong A and Eli M: DPP3 expression promotes cell proliferation and migration <em>in vitro</em> and tumour growth <em>in vivo</em>, which is associated with poor prognosis of oesophageal carcinoma. Oncol Rep 49: 9, 2023.
APA
Liu, J., Abudula, A., Yang, H., Xu, L., Nuerrula, Y., Bai, G. ... Eli, M. (2023). DPP3 expression promotes cell proliferation and migration <em>in vitro</em> and tumour growth <em>in vivo</em>, which is associated with poor prognosis of oesophageal carcinoma. Oncology Reports, 49, 9. https://doi.org/10.3892/or.2022.8446
MLA
Liu, J., Abudula, A., Yang, H., Xu, L., Nuerrula, Y., Bai, G., Tulahong, A., Eli, M."DPP3 expression promotes cell proliferation and migration <em>in vitro</em> and tumour growth <em>in vivo</em>, which is associated with poor prognosis of oesophageal carcinoma". Oncology Reports 49.1 (2023): 9.
Chicago
Liu, J., Abudula, A., Yang, H., Xu, L., Nuerrula, Y., Bai, G., Tulahong, A., Eli, M."DPP3 expression promotes cell proliferation and migration <em>in vitro</em> and tumour growth <em>in vivo</em>, which is associated with poor prognosis of oesophageal carcinoma". Oncology Reports 49, no. 1 (2023): 9. https://doi.org/10.3892/or.2022.8446
Copy and paste a formatted citation
x
Spandidos Publications style
Liu J, Abudula A, Yang H, Xu L, Nuerrula Y, Bai G, Tulahong A and Eli M: DPP3 expression promotes cell proliferation and migration <em>in vitro</em> and tumour growth <em>in vivo</em>, which is associated with poor prognosis of oesophageal carcinoma. Oncol Rep 49: 9, 2023.
APA
Liu, J., Abudula, A., Yang, H., Xu, L., Nuerrula, Y., Bai, G. ... Eli, M. (2023). DPP3 expression promotes cell proliferation and migration <em>in vitro</em> and tumour growth <em>in vivo</em>, which is associated with poor prognosis of oesophageal carcinoma. Oncology Reports, 49, 9. https://doi.org/10.3892/or.2022.8446
MLA
Liu, J., Abudula, A., Yang, H., Xu, L., Nuerrula, Y., Bai, G., Tulahong, A., Eli, M."DPP3 expression promotes cell proliferation and migration <em>in vitro</em> and tumour growth <em>in vivo</em>, which is associated with poor prognosis of oesophageal carcinoma". Oncology Reports 49.1 (2023): 9.
Chicago
Liu, J., Abudula, A., Yang, H., Xu, L., Nuerrula, Y., Bai, G., Tulahong, A., Eli, M."DPP3 expression promotes cell proliferation and migration <em>in vitro</em> and tumour growth <em>in vivo</em>, which is associated with poor prognosis of oesophageal carcinoma". Oncology Reports 49, no. 1 (2023): 9. https://doi.org/10.3892/or.2022.8446
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