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Article Open Access

LAPTM5 regulated by FOXP3 promotes the malignant phenotypes of breast cancer through activating the Wnt/β‑catenin pathway

  • Authors:
    • Sijia Han
    • Xueying Jin
    • Tianyu Hu
    • Feng Chi
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    Affiliations: Department of Oncology, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110022, P.R. China
    Copyright: © Han et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 60
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    Published online on: February 10, 2023
       https://doi.org/10.3892/or.2023.8497
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Abstract

Breast cancer remains the most common malignancy and the leading cause of cancer‑associated mortality in women worldwide. Lysosomal protein transmembrane 5 (LAPTM5), a lysosomal membrane protein, plays an important role in several human malignancies. However, the biological functions and mechanism of LAPTM5 in breast cancer remain unclear. In the present study, the potential tumor‑promoting effect of LAPTM5 was predicted by bioinformatics analysis. LAPTM5 was highly expressed in breast cancer clinical specimens. Moreover, in vitro studies demonstrated that cell proliferation, migration and invasion, as well as the process of epithelial‑mesenchymal transition (EMT) were promoted by LAPTM5 overexpression and were suppressed by LAPTM5 downregulation in vitro. The tumor‑promoting effects of LAPTM5 were also confirmed by xenograft tumor assay in vivo. It was found that the tumor‑promoting effects of LAPTM5 were partly dependent on the activation of the Wnt/β‑catenin signaling pathway. Furthermore, dual‑luciferase and chromatin immunoprecipitation assays verified that the transcription factor forkhead box protein 3 (FOXP3) directly bound to the promoter of LAPTM5 and negatively regulated its expression. Taken together, the present findings indicated that LAPTM5, negatively regulated by FOXP3, promoted the malignant phenotypes of breast cancer through activating the Wnt/β‑catenin signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Han S, Jin X, Hu T and Chi F: LAPTM5 regulated by FOXP3 promotes the malignant phenotypes of breast cancer through activating the Wnt/β‑catenin pathway. Oncol Rep 49: 60, 2023.
APA
Han, S., Jin, X., Hu, T., & Chi, F. (2023). LAPTM5 regulated by FOXP3 promotes the malignant phenotypes of breast cancer through activating the Wnt/β‑catenin pathway. Oncology Reports, 49, 60. https://doi.org/10.3892/or.2023.8497
MLA
Han, S., Jin, X., Hu, T., Chi, F."LAPTM5 regulated by FOXP3 promotes the malignant phenotypes of breast cancer through activating the Wnt/β‑catenin pathway". Oncology Reports 49.3 (2023): 60.
Chicago
Han, S., Jin, X., Hu, T., Chi, F."LAPTM5 regulated by FOXP3 promotes the malignant phenotypes of breast cancer through activating the Wnt/β‑catenin pathway". Oncology Reports 49, no. 3 (2023): 60. https://doi.org/10.3892/or.2023.8497
Copy and paste a formatted citation
x
Spandidos Publications style
Han S, Jin X, Hu T and Chi F: LAPTM5 regulated by FOXP3 promotes the malignant phenotypes of breast cancer through activating the Wnt/β‑catenin pathway. Oncol Rep 49: 60, 2023.
APA
Han, S., Jin, X., Hu, T., & Chi, F. (2023). LAPTM5 regulated by FOXP3 promotes the malignant phenotypes of breast cancer through activating the Wnt/β‑catenin pathway. Oncology Reports, 49, 60. https://doi.org/10.3892/or.2023.8497
MLA
Han, S., Jin, X., Hu, T., Chi, F."LAPTM5 regulated by FOXP3 promotes the malignant phenotypes of breast cancer through activating the Wnt/β‑catenin pathway". Oncology Reports 49.3 (2023): 60.
Chicago
Han, S., Jin, X., Hu, T., Chi, F."LAPTM5 regulated by FOXP3 promotes the malignant phenotypes of breast cancer through activating the Wnt/β‑catenin pathway". Oncology Reports 49, no. 3 (2023): 60. https://doi.org/10.3892/or.2023.8497
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