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NONHSAG028908.3 sponges miR‑34a‑5p to promote growth of colorectal cancer via targeting ALDOA

  • Authors:
    • Chuanzhuo Wang
    • He Xin
    • Guangxin Yan
    • Zhaoyu Liu
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    Affiliations: Department of Radiology, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 89
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    Published online on: March 15, 2023
       https://doi.org/10.3892/or.2023.8526
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Abstract

Colorectal cancer (CRC) is an aggressive tumor, whose development is considered to be modulated by certain long non‑coding RNAs (lncRNAs). Therefore, the aim of the present study was to investigate the regulatory mechanism of lncRNA NONHSAG028908.3 on CRC. Data from The Cancer Genome Atlas (TCGA) database revealed that NONHSAG028908.3 was increased in CRC tissues compared with normal tissues (P<0.001). The results of reverse transcription‑quantitative PCR indicated that NONHSAG028908.3 was upregulated in four types of CRC cells compared with that in NCM460, a normal colorectal cell line. MTT, BrdU, and flow cytometric assays were applied to evaluate CRC cell growth. The migratory and invasive abilities of CRC cells were detected using wound healing and Transwell assays. Silencing of NONHSAG028908.3 inhibited proliferation, migration, and invasion of CRC cells. A dual‑luciferase reporter assay demonstrated that NONHSAG028908.3 served as a sponge to combine with microRNA (miR)‑34a‑5p. MiR‑34a‑5p suppressed the aggressiveness of CRC cells. The effects induced by NONHSAG028908.3 knockdown were partly reversed by inhibition of miR‑34a‑5p. Furthermore, miR‑34a‑5p, a target of NONHSAG028908.3, modulated aldolase, fructose‑bisphosphate A (ALDOA) expression in a negative feedback manner. Suppression of NONHSAG028908.3 notably decreased ALDOA expression, which was rescued via silencing of miR‑34a‑5p. Moreover, suppression of ALDOA revealed the inhibitory action on CRC cell growth and migration. In summary, the data of the present study indicate that NONHSAG028908.3 may positively regulate ALDOA via sponging miR‑34a‑5p, thereby promoting malignant activities in CRC.
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Copy and paste a formatted citation
Spandidos Publications style
Wang C, Xin H, Yan G and Liu Z: NONHSAG028908.3 sponges miR‑34a‑5p to promote growth of colorectal cancer via targeting ALDOA. Oncol Rep 49: 89, 2023.
APA
Wang, C., Xin, H., Yan, G., & Liu, Z. (2023). NONHSAG028908.3 sponges miR‑34a‑5p to promote growth of colorectal cancer via targeting ALDOA. Oncology Reports, 49, 89. https://doi.org/10.3892/or.2023.8526
MLA
Wang, C., Xin, H., Yan, G., Liu, Z."NONHSAG028908.3 sponges miR‑34a‑5p to promote growth of colorectal cancer via targeting ALDOA". Oncology Reports 49.5 (2023): 89.
Chicago
Wang, C., Xin, H., Yan, G., Liu, Z."NONHSAG028908.3 sponges miR‑34a‑5p to promote growth of colorectal cancer via targeting ALDOA". Oncology Reports 49, no. 5 (2023): 89. https://doi.org/10.3892/or.2023.8526
Copy and paste a formatted citation
x
Spandidos Publications style
Wang C, Xin H, Yan G and Liu Z: NONHSAG028908.3 sponges miR‑34a‑5p to promote growth of colorectal cancer via targeting ALDOA. Oncol Rep 49: 89, 2023.
APA
Wang, C., Xin, H., Yan, G., & Liu, Z. (2023). NONHSAG028908.3 sponges miR‑34a‑5p to promote growth of colorectal cancer via targeting ALDOA. Oncology Reports, 49, 89. https://doi.org/10.3892/or.2023.8526
MLA
Wang, C., Xin, H., Yan, G., Liu, Z."NONHSAG028908.3 sponges miR‑34a‑5p to promote growth of colorectal cancer via targeting ALDOA". Oncology Reports 49.5 (2023): 89.
Chicago
Wang, C., Xin, H., Yan, G., Liu, Z."NONHSAG028908.3 sponges miR‑34a‑5p to promote growth of colorectal cancer via targeting ALDOA". Oncology Reports 49, no. 5 (2023): 89. https://doi.org/10.3892/or.2023.8526
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