Anti‑tumor effects of an aqueous extract of <em>Ecklonia cava</em> in BALB/cKorl syngeneic mice using colon carcinoma CT26 cells

Gong,Jeong Eun; Kim,Ji Eun; Park,So Hae; Lee,Su Jin; Choi,Yun Ju; Choi,Sun Il; Choi,Young Whan; Lee,Hee Seob; Hong,Jin Tae; Hwang,Dae Youn

doi:10.3892/or.2023.8565

Anti‑tumor effects of an aqueous extract of Ecklonia cava in BALB/cKorl syngeneic mice using colon carcinoma CT26 cells

Authors:
- Jeong Eun Gong
- Ji Eun Kim
- So Hae Park
- Su Jin Lee
- Yun Ju Choi
- Sun Il Choi
- Young Whan Choi
- Hee Seob Lee
- Jin Tae Hong
- Dae Youn Hwang
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Affiliations: Department of Biomaterials Science (BK21 FOUR Program), College of Natural Resources & Life Science/Life and Industry Convergence Research Institute/Laboratory Animal Resources Center, Pusan National University, Miryang 50463, Republic of Korea, School of Pharmacy, Henan University, Henan 475004, P.R. China, Department of Horticultural Bioscience, College of Natural Resources and Life Science, Pusan National University, Miryang 50463, Republic of Korea, Department of Food Science and Nutrition, College of Human Ecology, Pusan National University, Busan 46241, Republic of Korea, College of Pharmacy, Chungbuk National University, Chungju 28644, Republic of Korea
Published online on: May 8, 2023 https://doi.org/10.3892/or.2023.8565
Article Number: 128
Copyright: © Gong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Ecklonia cava (E. cava) is well known as one of edible alga that contains various unique polyphenols. The anti‑tumor activity of an aqueous extract of E. cava (AEC) against colon carcinoma was evaluated by analyzing the alterations in tumor growth, histopathological structure and molecular mechanisms in CT26 tumor‑bearing BALB/cKorl syngeneic mice after administrating AEC for five weeks. AEC contained high total phenolic contents and demonstrated significant scavenging activity against 2,2‑diphenyl‑1‑picrylhydrazyl radicals. Marked anti‑tumor effects were demonstrated in the AEC‑treated CT26 cells. In the in vivo syngeneic model, the AEC treatment decreased the volume and weight of CT26 tumors, and expanded the necrotic region in the hematoxylin and eosin stained tumor sections. The inhibitory effects of AEC on tumor growth were reflected by the increased level of apoptotic proteins, inhibition of cell proliferation, suppression of metastasis ability and increase in tumor‑suppressing activity in CT26 tumor‑bearing BALB/cKorl syngeneic mice. The potential function of phlorotannin (PT), one of the primary active compounds in AEC, was demonstrated by the increased cytotoxicity, apoptosis and suppression of cell proliferation in PT‑treated CT26 cells. Overall, the results of the present study provide novel scientific evidence that AEC can suppress the growth of CT26 colon cancer by activating apoptosis, suppressing cell proliferation, inhibiting cell migration and enhancing the tumor‑suppressing activity.

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