Open Access

RB1‑promoter methylation in glioblastoma: A rare event in glioblastoma

  • Authors:
    • Steffi Urbschat
    • Gerrit Breitfelder
    • Mohamed Henia
    • Walter Schulz‑Schaeffer
    • Christoph Sippl
    • Joachim Oertel
    • Ralf Ketter
  • View Affiliations

  • Published online on: June 2, 2023     https://doi.org/10.3892/or.2023.8580
  • Article Number: 143
  • Copyright: © Urbschat et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The retinoblastoma gene (RB1) is a tumor suppressor gene that serves a key role in the development of numerous tumor diseases that can be downregulated by DNA methylation within its promoter region. The present study analyzed the methylation status of the RB1 promoter of 85 glioblastomas to assess its role in this tumor. To elucidate the underlying mechanism, RB1 promoter methylation was evaluated using methylation‑specific PCR with subsequent evaluation of the results via gel electrophoresis using ethidium bromide. Of the 85 samples analyzed, only one demonstrated RB1‑promoter methylation. While there are contradictory results on this matter in the literature, this study is, to the best of our knowledge, the largest on this topic to date as well as the first to use the WHO 2016 classification. The results of the present indicated that the RB1 promoter methylation does not serve a role in the development and progression of glioblastoma.
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July-2023
Volume 50 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Urbschat S, Breitfelder G, Henia M, Schulz‑Schaeffer W, Sippl C, Oertel J and Ketter R: RB1‑promoter methylation in glioblastoma: A rare event in glioblastoma. Oncol Rep 50: 143, 2023
APA
Urbschat, S., Breitfelder, G., Henia, M., Schulz‑Schaeffer, W., Sippl, C., Oertel, J., & Ketter, R. (2023). RB1‑promoter methylation in glioblastoma: A rare event in glioblastoma. Oncology Reports, 50, 143. https://doi.org/10.3892/or.2023.8580
MLA
Urbschat, S., Breitfelder, G., Henia, M., Schulz‑Schaeffer, W., Sippl, C., Oertel, J., Ketter, R."RB1‑promoter methylation in glioblastoma: A rare event in glioblastoma". Oncology Reports 50.1 (2023): 143.
Chicago
Urbschat, S., Breitfelder, G., Henia, M., Schulz‑Schaeffer, W., Sippl, C., Oertel, J., Ketter, R."RB1‑promoter methylation in glioblastoma: A rare event in glioblastoma". Oncology Reports 50, no. 1 (2023): 143. https://doi.org/10.3892/or.2023.8580