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Inhibition of protein arginine deiminase II suppresses retinoblastoma in orthotopic transplantation in mice

  • Authors:
    • Sojin Kim
    • Yong Keun Song
    • Chang Sik Cho
    • Hyo Jung Kim
    • Sungsoon Fang
    • Dong Hyun Jo
    • Hyunkyung Kim
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry and Molecular Biology, Korea University College of Medicine, Seoul 02841, Republic of Korea, Department of Medicine, Seoul National University College of Medicine, Seoul 03080, Republic of Korea, Fight against Angiogenesis‑Related Blindness Laboratory, Biomedical Research Institute, Seoul National University Hospital, Seoul 03080, Republic of Korea, Department of Anatomy and Cell Biology, Seoul National University College of Medicine, Seoul 03080, Republic of Korea, Severance Biomedical Science Institute, Gangnam Severance Hospital; Graduate School of Medical Science, Brain Korea 21 Project, Yonsei University College of Medicine, Seoul 06273, Republic of Korea
    Copyright: © Kim et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 146
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    Published online on: June 13, 2023
       https://doi.org/10.3892/or.2023.8583
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Abstract

Chemotherapies are used for treating retinoblastoma; however, numerous patients suffer from recurrence or symptoms due to chemotherapy, which emphasizes the need for alternative therapeutic strategies. The present study demonstrated that protein arginine deiminase Ⅱ (PADI2) was highly expressed in human and mouse retinoblastoma tissues due to the overexpression of E2 factor (E2F). By inhibiting PADI2 activity, the expression of phosphorylated AKT was reduced, and cleaved poly (ADP‑ribose) polymerase level was increased, leading to induced apoptosis. Similar results were obtained in orthotopic mouse models with reduced tumor volumes. In addition, BB‑Cl‑amidine showed low toxicity in vivo. These results suggested that PADI2 inhibition has potential clinical translation. Furthermore, the present study highlights the potential of epigenetic approaches to target RB1‑deficient mutations at the molecular level. The current findings provide new insights into the importance of retinoblastoma intervention by managing PADI2 activity according to the treatment of specific inhibitors and depletion approaches in vitro and in orthotopic mouse models.
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Copy and paste a formatted citation
Spandidos Publications style
Kim S, Song YK, Cho CS, Kim HJ, Fang S, Jo DH and Kim H: Inhibition of protein arginine deiminase II suppresses retinoblastoma in orthotopic transplantation in mice. Oncol Rep 50: 146, 2023.
APA
Kim, S., Song, Y.K., Cho, C.S., Kim, H.J., Fang, S., Jo, D.H., & Kim, H. (2023). Inhibition of protein arginine deiminase II suppresses retinoblastoma in orthotopic transplantation in mice. Oncology Reports, 50, 146. https://doi.org/10.3892/or.2023.8583
MLA
Kim, S., Song, Y. K., Cho, C. S., Kim, H. J., Fang, S., Jo, D. H., Kim, H."Inhibition of protein arginine deiminase II suppresses retinoblastoma in orthotopic transplantation in mice". Oncology Reports 50.1 (2023): 146.
Chicago
Kim, S., Song, Y. K., Cho, C. S., Kim, H. J., Fang, S., Jo, D. H., Kim, H."Inhibition of protein arginine deiminase II suppresses retinoblastoma in orthotopic transplantation in mice". Oncology Reports 50, no. 1 (2023): 146. https://doi.org/10.3892/or.2023.8583
Copy and paste a formatted citation
x
Spandidos Publications style
Kim S, Song YK, Cho CS, Kim HJ, Fang S, Jo DH and Kim H: Inhibition of protein arginine deiminase II suppresses retinoblastoma in orthotopic transplantation in mice. Oncol Rep 50: 146, 2023.
APA
Kim, S., Song, Y.K., Cho, C.S., Kim, H.J., Fang, S., Jo, D.H., & Kim, H. (2023). Inhibition of protein arginine deiminase II suppresses retinoblastoma in orthotopic transplantation in mice. Oncology Reports, 50, 146. https://doi.org/10.3892/or.2023.8583
MLA
Kim, S., Song, Y. K., Cho, C. S., Kim, H. J., Fang, S., Jo, D. H., Kim, H."Inhibition of protein arginine deiminase II suppresses retinoblastoma in orthotopic transplantation in mice". Oncology Reports 50.1 (2023): 146.
Chicago
Kim, S., Song, Y. K., Cho, C. S., Kim, H. J., Fang, S., Jo, D. H., Kim, H."Inhibition of protein arginine deiminase II suppresses retinoblastoma in orthotopic transplantation in mice". Oncology Reports 50, no. 1 (2023): 146. https://doi.org/10.3892/or.2023.8583
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