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Article Open Access

MBNL1‑AS1 attenuates tumor cell proliferation by regulating the miR‑29c‑3p/BVES signal in colorectal cancer

  • Authors:
    • Wang-Sheng Chen
    • Xu Zhang
    • Zheng-Fei Zhao
    • Xiang-Ming Che
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shanxi 710061, P.R. China, Department of Geriatrics, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, P.R. China, Department of General Surgery (Gastrointestinal Surgery), The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 191
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    Published online on: September 13, 2023
       https://doi.org/10.3892/or.2023.8628
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Abstract

Dysregulation of long non‑coding RNAs (lncRNAs) is involved in the development of colorectal cancer (CRC). In the present study, the identification of muscle blind like splicing regulator 1 antisense RNA 1 (MBNL1‑AS1) lncRNA was reported. Firstly, Cell Counting Kit‑8, EdU and colony formation assays were uesed to explore the role of MBNL1‑AS1 in regulating the proliferation of CRC cells. According to TCGA database, it was found that MBNL1‑AS1 was correlated with microRNA (miR)‑29c‑3p and blood vessel epicardial substance (BVES) expression in CRC cells. Then, the regulation among MBNL1‑AS1, miR‑29C‑3P and BVES was detected by dual luciferase reporter assay and the function of MBNL1‑AS1/miR‑29C‑3P/BVES axis was explored by rescue assay. The results demonstrated that MBNL1‑AS1 expression was decreased in CRC and was associated with the size of tumors derived from patients with CRC. Functionally, the upregulation of MBNL1‑AS1 suppressed CRC cell proliferation in vitro and inhibited tumor growth in vivo, while knockdown of MBNL1‑AS1 expression caused the opposite effects. MBNL1‑AS1 expression correlated with BVES expression in CRC tissues and MBNL1‑AS1 enhanced the stability of BVES mRNA by functioning as a competing endogenous RNA to sponge miR‑29c‑3p; the latter directly targeted MBNL1‑AS1 and BVES mRNA 3'UTR. Collectively, the results indicated that MBNL1‑AS1 suppressed CRC cell proliferation by regulating miR‑29c‑3p/BVES signaling, suggesting that the MBNL1‑AS1/miR‑29c‑3p/BVES axis may be a potential therapeutic target for CRC.
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Copy and paste a formatted citation
Spandidos Publications style
Chen W, Zhang X, Zhao Z and Che X: MBNL1‑AS1 attenuates tumor cell proliferation by regulating the miR‑29c‑3p/BVES signal in colorectal cancer. Oncol Rep 50: 191, 2023.
APA
Chen, W., Zhang, X., Zhao, Z., & Che, X. (2023). MBNL1‑AS1 attenuates tumor cell proliferation by regulating the miR‑29c‑3p/BVES signal in colorectal cancer. Oncology Reports, 50, 191. https://doi.org/10.3892/or.2023.8628
MLA
Chen, W., Zhang, X., Zhao, Z., Che, X."MBNL1‑AS1 attenuates tumor cell proliferation by regulating the miR‑29c‑3p/BVES signal in colorectal cancer". Oncology Reports 50.4 (2023): 191.
Chicago
Chen, W., Zhang, X., Zhao, Z., Che, X."MBNL1‑AS1 attenuates tumor cell proliferation by regulating the miR‑29c‑3p/BVES signal in colorectal cancer". Oncology Reports 50, no. 4 (2023): 191. https://doi.org/10.3892/or.2023.8628
Copy and paste a formatted citation
x
Spandidos Publications style
Chen W, Zhang X, Zhao Z and Che X: MBNL1‑AS1 attenuates tumor cell proliferation by regulating the miR‑29c‑3p/BVES signal in colorectal cancer. Oncol Rep 50: 191, 2023.
APA
Chen, W., Zhang, X., Zhao, Z., & Che, X. (2023). MBNL1‑AS1 attenuates tumor cell proliferation by regulating the miR‑29c‑3p/BVES signal in colorectal cancer. Oncology Reports, 50, 191. https://doi.org/10.3892/or.2023.8628
MLA
Chen, W., Zhang, X., Zhao, Z., Che, X."MBNL1‑AS1 attenuates tumor cell proliferation by regulating the miR‑29c‑3p/BVES signal in colorectal cancer". Oncology Reports 50.4 (2023): 191.
Chicago
Chen, W., Zhang, X., Zhao, Z., Che, X."MBNL1‑AS1 attenuates tumor cell proliferation by regulating the miR‑29c‑3p/BVES signal in colorectal cancer". Oncology Reports 50, no. 4 (2023): 191. https://doi.org/10.3892/or.2023.8628
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