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Article

Wnt/β‑catenin signaling is a novel therapeutic target for tumor suppressor CYLD‑silenced glioblastoma cells

  • Authors:
    • Ayumi Kanemaru
    • Yuki Ito
    • Michiko Yamaoka
    • Yuki Shirakawa
    • Kou Yonemaru
    • Shunsuke Miyake
    • Misaki Ando
    • Masako Ota
    • Takeshi Masuda
    • Akitake Mukasa
    • Jian-Dong Li
    • Hideyuki Saito
    • Takuichiro Hide
    • Hirofumi Jono
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Pharmaceutical Sciences, Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto 860‑8556, Japan, Department of Therapy Development and Innovation for Immune Disorders and Cancers, Graduate School of Medicine, Juntendo University, Tokyo 113‑8421, Japan, Department of Pharmaceutical Microbiology, Faculty of Life Sciences, Kumamoto University, Kumamoto 862‑0973, Japan, Department of Neurosurgery, Faculty of Life Sciences, Kumamoto University, Kumamoto 860‑8556, Japan, Center for Inflammation, Immunity and Infection, Institute for Biomedical Sciences, Georgia State University, Atlanta, GA 30303, USA, Department of Neurosurgery, Kitasato University School of Medicine, Sagamihara, Kanagawa 252‑0375, Japan
  • Article Number: 201
    |
    Published online on: September 29, 2023
       https://doi.org/10.3892/or.2023.8638
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Abstract

Tumor suppressor cylindromatosis (CYLD) dysfunction by its downregulation is significantly associated with poor prognosis in patients with glioblastoma (GBM), the most aggressive and malignant type of glioma. However, no effective treatment is currently available for patients with CYLD‑downregulated GBM. The aim of the present study was to identify the crucial cell signaling pathways and novel therapeutic targets for CYLD downregulation in GBM cells. CYLD knockdown in GBM cells induced GBM malignant characteristics, such as proliferation, metastasis, and GBM stem‑like cell (GSC) formation. Comprehensive proteomic analysis and RNA sequencing data from the tissues of patients with GBM revealed that Wnt/β‑catenin signaling was significantly activated by CYLD knockdown in patients with GBM. Furthermore, a Wnt/β‑catenin signaling inhibitor suppressed all CYLD knockdown‑induced malignant characteristics of GBM. Taken together, the results of the present study revealed that Wnt/β‑catenin signaling is responsible for CYLD silencing‑induced GBM malignancy; therefore, targeting Wnt/β‑catenin may be effective for the treatment of CYLD‑negative patients with GBM with poor prognosis.
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Copy and paste a formatted citation
Spandidos Publications style
Kanemaru A, Ito Y, Yamaoka M, Shirakawa Y, Yonemaru K, Miyake S, Ando M, Ota M, Masuda T, Mukasa A, Mukasa A, et al: Wnt/β‑catenin signaling is a novel therapeutic target for tumor suppressor CYLD‑silenced glioblastoma cells. Oncol Rep 50: 201, 2023.
APA
Kanemaru, A., Ito, Y., Yamaoka, M., Shirakawa, Y., Yonemaru, K., Miyake, S. ... Jono, H. (2023). Wnt/β‑catenin signaling is a novel therapeutic target for tumor suppressor CYLD‑silenced glioblastoma cells. Oncology Reports, 50, 201. https://doi.org/10.3892/or.2023.8638
MLA
Kanemaru, A., Ito, Y., Yamaoka, M., Shirakawa, Y., Yonemaru, K., Miyake, S., Ando, M., Ota, M., Masuda, T., Mukasa, A., Li, J., Saito, H., Hide, T., Jono, H."Wnt/β‑catenin signaling is a novel therapeutic target for tumor suppressor CYLD‑silenced glioblastoma cells". Oncology Reports 50.5 (2023): 201.
Chicago
Kanemaru, A., Ito, Y., Yamaoka, M., Shirakawa, Y., Yonemaru, K., Miyake, S., Ando, M., Ota, M., Masuda, T., Mukasa, A., Li, J., Saito, H., Hide, T., Jono, H."Wnt/β‑catenin signaling is a novel therapeutic target for tumor suppressor CYLD‑silenced glioblastoma cells". Oncology Reports 50, no. 5 (2023): 201. https://doi.org/10.3892/or.2023.8638
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Spandidos Publications style
Kanemaru A, Ito Y, Yamaoka M, Shirakawa Y, Yonemaru K, Miyake S, Ando M, Ota M, Masuda T, Mukasa A, Mukasa A, et al: Wnt/β‑catenin signaling is a novel therapeutic target for tumor suppressor CYLD‑silenced glioblastoma cells. Oncol Rep 50: 201, 2023.
APA
Kanemaru, A., Ito, Y., Yamaoka, M., Shirakawa, Y., Yonemaru, K., Miyake, S. ... Jono, H. (2023). Wnt/β‑catenin signaling is a novel therapeutic target for tumor suppressor CYLD‑silenced glioblastoma cells. Oncology Reports, 50, 201. https://doi.org/10.3892/or.2023.8638
MLA
Kanemaru, A., Ito, Y., Yamaoka, M., Shirakawa, Y., Yonemaru, K., Miyake, S., Ando, M., Ota, M., Masuda, T., Mukasa, A., Li, J., Saito, H., Hide, T., Jono, H."Wnt/β‑catenin signaling is a novel therapeutic target for tumor suppressor CYLD‑silenced glioblastoma cells". Oncology Reports 50.5 (2023): 201.
Chicago
Kanemaru, A., Ito, Y., Yamaoka, M., Shirakawa, Y., Yonemaru, K., Miyake, S., Ando, M., Ota, M., Masuda, T., Mukasa, A., Li, J., Saito, H., Hide, T., Jono, H."Wnt/β‑catenin signaling is a novel therapeutic target for tumor suppressor CYLD‑silenced glioblastoma cells". Oncology Reports 50, no. 5 (2023): 201. https://doi.org/10.3892/or.2023.8638
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