MicroRNA‑606 inhibits the growth and metastasis of triple‑negative breast cancer by targeting Stanniocalcin 1

Choi,Sujin; An,Hyun-Ju; Yeo,Hyun Jeong; Sung,Min-Ji; Oh,Jisu; Lee,Kwanbum; Lee,Seung Ah; Kim,Seung Ki; Kim,Junhan; Kim,Isaac; Lee,Soonchul

doi:10.3892/or.2023.8661

MicroRNA‑606 inhibits the growth and metastasis of triple‑negative breast cancer by targeting Stanniocalcin 1

Authors:
- Sujin Choi
- Hyun-Ju An
- Hyun Jeong Yeo
- Min-Ji Sung
- Jisu Oh
- Kwanbum Lee
- Seung Ah Lee
- Seung Ki Kim
- Junhan Kim
- Isaac Kim
- Soonchul Lee
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Affiliations: Department of Orthopedic Surgery, CHA Bundang Medical Center, CHA University School of Medicine, Seongnam, Gyeonggi 13488, Republic of Korea, Division of Hemato‑Oncology, Department of Internal Medicine, Yongin Severance Hospital, Yonsei University College of Medicine, Yongin, Gyeonggi 16995, Republic of Korea, Department of General Surgery, CHA Bundang Medical Center, CHA University School of Medicine, Seongnam, Gyeonggi 13488, Republic of Korea
Published online on: November 15, 2023 https://doi.org/10.3892/or.2023.8661
Article Number: 2
Copyright: © Choi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Triple‑negative breast cancer (TNBC) is associated with a poor prognosis; however, treatments for TNBC are limited, with poor outcomes. MicroRNAs (miRNAs/miRs) are small non‑coding RNA molecules that are able to regulate gene expression. The present study aimed to identify differentially expressed miRNAs in patients with breast cancer, and to investigate the functional role of the identified miRNA targets and their effects in vitro and in vivo. Transfection with miR‑606 suppressed TNBC cell proliferation, migration, invasion and tumor sphere‑forming ability, as determined using trypan blue, Transwell and sphere formation assays. Moreover, miR‑606 induced the apoptosis of TNBC cells, as determined by flow cytometric analysis. Furthermore, intratumoral injections of miR‑606 mimics suppressed tumor growth in MDA‑MB‑231 xenografts. In addition, MDA‑MB‑231 cells transfected with miR‑606 mimics exhibited decreased lung metastatic nodules in a mouse tail vein injection model. Notably, miR‑606 and STC1 expression had opposing effects on the overall survival of patients with TNBC. The results of the present study suggested a novel tumor suppressor function for miR‑606 in TNBC, thus indicating its potential application in the development of anticancer miRNA therapeutics.

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