Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1021-335X Online ISSN: 1791-2431
Journal Cover
March-2024 Volume 51 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
March-2024 Volume 51 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML

  • Supplementary Files
    • Supplementary_Data1.pdf
    • Supplementary_Data2.pdf
Article Open Access

Evaluation of DNA methylation levels of SEPT9 and SHOX2 in plasma of patients with head and neck squamous cell carcinoma using droplet digital PCR

  • Authors:
    • Ilaria Grossi
    • Claudia Assoni
    • Luigi Lorini
    • Davide Smussi
    • Cristina Gurizzan
    • Salvatore Grisanti
    • Alberto Paderno
    • Davide Mattavelli
    • Cesare Piazza
    • Iulia Andreea Pelisenco
    • Giuseppina De Petro
    • Alessandro Salvi
    • Paolo Bossi
  • View Affiliations / Copyright

    Affiliations: Division of Biology and Genetics, Department of Molecular and Translational Medicine, University of Brescia, I‑25123 Brescia, Italy, Unit of Medical Oncology, Department of Medical and Surgical Specialties, Radiological Sciences and Public Health, ASST Spedali Civili of Brescia, University of Brescia, I‑25123 Brescia, Italy, Unit of Otorhinolaryngology‑Head and Neck Surgery, Department of Medical and Surgical Specialties, Radiological Sciences and Public Health, ASST Spedali Civili of Brescia, University of Brescia, I‑25123 Brescia, Italy
    Copyright: © Grossi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 52
    |
    Published online on: January 31, 2024
       https://doi.org/10.3892/or.2024.8711
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Head and neck squamous cell carcinoma (HNSCC) is the seventh most commonly diagnosed cancer globally. HNSCC develops from the mucosa of the oral cavity, pharynx and larynx. Methylation levels of septin 9 (SEPT9) and short stature homeobox 2 (SHOX2) genes in circulating cell‑free DNA (ccfDNA) are considered epigenetic biomarkers and have shown predictive value in preliminary reports in HNSCC. Liquid biopsy is a non‑invasive procedure that collects tumor‑derived molecules, including ccfDNA. In the present study, a droplet digital PCR (ddPCR)‑based assay was developed to detect DNA methylation levels of circulating SEPT9 and SHOX2 in the plasma of patients with HNSCC. The assay was first set up using commercial methylated and unmethylated DNA. The dynamic changes in the methylation levels of SEPT9 and SHOX2 were then quantified in 20 patients with HNSCC during follow‑up. The results highlighted: i) The ability of the ddPCR‑based assay to detect very low copies of methylated molecules; ii) the significant decrease in SEPT9 and SHOX2 methylation levels in the plasma of patients with HNSCC at the first time points of follow‑up with respect to T0; iii) a different trend of longitudinally DNA methylation variations in small groups of stratified patients. The absolute and precise quantification of SEPT9 and SHOX2 methylation levels in HNSCC may be useful for studies with translational potential.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

View References

1 

Mody MD, Rocco JW, Yom SS, Haddad RI and Saba NF: Head and neck cancer. Lancet. 398:2289–2299. 2021. View Article : Google Scholar : PubMed/NCBI

2 

Sung H, Ferlay J, Siegel RL, Laversanne M, Soerjomataram I, Jemal A and Bray F: Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 71:209–249. 2021. View Article : Google Scholar : PubMed/NCBI

3 

Barsouk A, Aluru JS, Rawla P, Saginala K and Barsouk A: Epidemiology, risk factors, and prevention of head and neck squamous cell carcinoma. Med Sci (Basel). 11:422023.PubMed/NCBI

4 

Muzaffar J, Bari S, Kirtane K and Chung CH: Recent advances and future directions in clinical management of head and neck squamous cell carcinoma. Cancers (Basel). 13:3382021. View Article : Google Scholar : PubMed/NCBI

5 

Sánchez-Herrero E, Serna-Blasco R, Robado de Lope L, González-Rumayor V, Romero A and Provencio M: Circulating tumor DNA as a cancer biomarker: An overview of biological features and factors that may impact on ctDNA analysis. Front Oncol. 12:9432532022. View Article : Google Scholar : PubMed/NCBI

6 

Caputo V, Ciardiello F, Corte CMD, Martini G, Troiani T and Napolitano S: Diagnostic value of liquid biopsy in the era of precision medicine: 10 Years of clinical evidence in cancer. Explor Target Antitumor Ther. 4:102–138. 2023. View Article : Google Scholar : PubMed/NCBI

7 

Kogo R, Manako T, Iwaya T, Nishizuka S, Hiraki H, Sasaki Y, Idogawa M, Tokino T, Koide A, Komune N, et al: Individualized circulating tumor DNA monitoring in head and neck squamous cell carcinoma. Cancer Med. 11:3960–3968. 2022. View Article : Google Scholar : PubMed/NCBI

8 

Khan KH, Cunningham D, Werner B, Vlachogiannis G, Spiteri I, Heide T, Mateos JF, Vatsiou A, Lampis A, Damavandi MD, et al: Longitudinal liquid biopsy and mathematical modeling of clonal evolution forecast time to treatment failure in the PROSPECT-C phase II colorectal cancer clinical trial. Cancer Discov. 8:1270–1285. 2018. View Article : Google Scholar : PubMed/NCBI

9 

Abeni E, Grossi I, Marchina E, Coniglio A, Incardona P, Cavalli P, Zorzi F, Chiodera PL, Paties CT, Crosatti M, et al: DNA methylation variations in familial female and male breast cancer. Oncol Lett. 21:4682021. View Article : Google Scholar : PubMed/NCBI

10 

Abeni E, Salvi A, Marchina E, Traversa M, Arici B and De Petro G: Sorafenib induces variations of the DNA methylome in HA22T/VGH human hepatocellular carcinoma-derived cells. Int J Oncol. 51:128–144. 2017. View Article : Google Scholar : PubMed/NCBI

11 

Markou Α, Londra D, Tserpeli V, Kollias I, Tsaroucha E, Vamvakaris I, Potaris K, Pateras I, Kotsakis Α, Georgoulias V and Lianidou Ε: DNA methylation analysis of tumor suppressor genes in liquid biopsy components of early stage NSCLC: A promising tool for early detection. Clin Epigenetics. 14:612022. View Article : Google Scholar : PubMed/NCBI

12 

Schröck A, Leisse A, de Vos L, Gevensleben H, Dröge F, Franzen A, Wachendörfer M, Schröck F, Ellinger J, Teschke M, et al: Free-circulating methylated DNA in blood for diagnosis, staging, prognosis, and monitoring of head and neck squamous cell carcinoma patients: An observational prospective cohort study. Clin Chem. 63:1288–1296. 2017. View Article : Google Scholar : PubMed/NCBI

13 

Sun J, Zheng MY, Li YW and Zhang SW: Structure and function of Septin 9 and its role in human malignant tumors. World J Gastrointest Oncol. 12:619–631. 2020. View Article : Google Scholar : PubMed/NCBI

14 

Semaan A, van Ellen A, Meller S, Bergheim D, Branchi V, Lingohr P, Goltz D, Kalff JC, Kristiansen G, Matthaei H, et al: SEPT9 and SHOX2 DNA methylation status and its utility in the diagnosis of colonic adenomas and colorectal adenocarcinomas. Clin Epigenetics. 8:1002016. View Article : Google Scholar : PubMed/NCBI

15 

Wasserkort R, Kalmar A, Valcz G, Spisak S, Krispin M, Toth K, Tulassay Z, Sledziewski AZ and Molnar B: Aberrant septin 9 DNA methylation in colorectal cancer is restricted to a single CpG island. BMC Cancer. 13:3982013. View Article : Google Scholar : PubMed/NCBI

16 

Matsui S, Kagara N, Mishima C, Naoi Y, Shimoda M, Shimomura A, Shimazu K, Kim SJ and Noguchi S: Methylation of the SEPT9-v2 promoter as a novel marker for the detection of circulating tumor DNA in breast cancer patients. Oncol Rep. 36:2225–2235. 2016. View Article : Google Scholar : PubMed/NCBI

17 

Jiang Y, Liu L, Xiang Q, He X, Wang Y, Zhou D, Zou C, Chen Q, Peng M, He J, et al: SEPT9-v2, frequently silenced by promoter hypermethylation, exerts anti-tumor functions through inactivation of Wnt/β-catenin signaling pathway via miR92b-3p/FZD10 in nasopharyngeal carcinoma cells. Clin Epigenetics. 12:412020. View Article : Google Scholar : PubMed/NCBI

18 

Li Y, Song L, Gong Y and He B: Detection of colorectal cancer by DNA methylation biomarker SEPT9: Past, present and future. Biomark Med. 8:755–769. 2014. View Article : Google Scholar : PubMed/NCBI

19 

Verdier-Pinard P, Salaun D, Bouguenina H, Shimada S, Pophillat M, Audebert S, Agavnian E, Coslet S, Charafe-Jauffret E, Tachibana T and Badache A: Septin 9_i2 is downregulated in tumors, impairs cancer cell migration and alters subnuclear actin filaments. Sci Rep. 7:449762017. View Article : Google Scholar : PubMed/NCBI

20 

Zhou X, Lu X, Wu H, Liu J and Huang H: Diagnostic performance of SHOX2 promoter methylation as biomarker for lung cancer identification: A meta-analysis update. Thorac Cancer. 12:3327–3332. 2021. View Article : Google Scholar : PubMed/NCBI

21 

Song L, Yu H and Li Y: Diagnosis of lung cancer by SHOX2 gene methylation assay. Mol Diagn Ther. 19:159–167. 2015. View Article : Google Scholar : PubMed/NCBI

22 

Gao H, Yang J, He L, Wang W, Liu Y, Hu Y, Ge M, Ding J and Ye Q: The diagnostic potential of SHOX2 and RASSF1A DNA methylation in early lung adenocarcinoma. Front Oncol. 12:8490242022. View Article : Google Scholar : PubMed/NCBI

23 

Zhang YA, Zhou Y, Luo X, Song K, Ma X, Sathe A, Girard L, Xiao G and Gazdar AF: SHOX2 is a potent independent biomarker to predict survival of WHO grade II–III diffuse gliomas. EBioMedicine. 13:80–89. 2016. View Article : Google Scholar : PubMed/NCBI

24 

de Vos L, Gevensleben H, Schröck A, Franzen A, Kristiansen G, Bootz F and Dietrich D: Comparison of quantification algorithms for circulating cell-free DNA methylation biomarkers in blood plasma from cancer patients. Clin Epigenetics. 9:1252017. View Article : Google Scholar : PubMed/NCBI

25 

Hindson CM, Chevillet JR, Briggs HA, Gallichotte EN, Ruf IK, Hindson BJ, Vessella RL and Tewari M: Absolute quantification by droplet digital PCR versus analog real-time PCR. Nat Methods. 10:1003–1005. 2013. View Article : Google Scholar : PubMed/NCBI

26 

Camuzi D, Buexm LA, Lourenço SQC, Esposti DD, Cuenin C, Lopes MSA, Manara F, Talukdar FR, Herceg Z, Ribeiro Pinto LF and Soares-Lima SC: HPV infection leaves a DNA methylation signature in oropharyngeal cancer affecting both coding genes and transposable elements. Cancers (Basel). 13:36212021. View Article : Google Scholar : PubMed/NCBI

27 

Wiencke JK, Bracci PM, Hsuang G, Zheng S, Hansen H, Wrensch MR, Rice T, Eliot M and Kelsey KT: A comparison of DNA methylation specific droplet digital PCR (ddPCR) and real time qPCR with flow cytometry in characterizing human T cells in peripheral blood. Epigenetics. 9:1360–1365. 2014. View Article : Google Scholar : PubMed/NCBI

28 

Van Wesenbeeck L, Janssens L, Meeuws H, Lagatie O and Stuyver L: Droplet digital PCR is an accurate method to assess methylation status on FFPE samples. Epigenetics. 13:207–213. 2018. View Article : Google Scholar : PubMed/NCBI

29 

Hindson BJ, Ness KD, Masquelier DA, Belgrader P, Heredia NJ, Makarewicz AJ, Bright IJ, Lucero MY, Hiddessen AL, Legler TC, et al: High-throughput droplet digital PCR system for absolute quantitation of DNA copy number. Anal Chem. 83:8604–8610. 2011. View Article : Google Scholar : PubMed/NCBI

30 

Manganelli M, Grossi I, Ferracin M, Guerriero P, Negrini M, Ghidini M, Senti C, Ratti M, Pizzo C, Passalacqua R, et al: Longitudinal circulating levels of miR-23b-3p, miR-126-3p and lncRNA GAS5 in HCC patients treated with sorafenib. Biomedicines. 9:8132021. View Article : Google Scholar : PubMed/NCBI

31 

van Ginkel JH, Huibers MMH, van Es RJJ, de Bree R and Willems SM: Droplet digital PCR for detection and quantification of circulating tumor DNA in plasma of head and neck cancer patients. BMC Cancer. 17:4282017. View Article : Google Scholar : PubMed/NCBI

32 

Fung SYH, Chan KCA, Wong EWY, Ng CWK, Cho R, Yeung ZWC, Lam JWK and Chan JYK: Droplet digital PCR of tumor suppressor gene methylation in serial oral rinses of patients with head and neck squamous cell carcinoma. Head Neck. 43:1812–1822. 2021. View Article : Google Scholar : PubMed/NCBI

33 

Huang SH and O'Sullivan B: Overview of the 8th edition TNM classification for head and neck cancer. Curr Treat Options Oncol. 18:402017. View Article : Google Scholar : PubMed/NCBI

34 

Ku JL, Jeon YK and Park JG: Methylation-specific PCR. Methods Mol Biol. 791:23–32. 2011. View Article : Google Scholar : PubMed/NCBI

35 

Huang Z, Bassil CF and Murphy SK: Methylation-specific PCR. Methods Mol Biol. 1049:75–82. 2013. View Article : Google Scholar : PubMed/NCBI

36 

Manganelli M, Grossi I, Corsi J, D'Agostino VG, Jurikova K, Cusanelli E, Molfino S, Portolani N, Salvi A and De Petro G: Expression of cellular and extracellular TERRA, TERC and TERT in hepatocellular carcinoma. Int J Mol Sci. 23:61832022. View Article : Google Scholar : PubMed/NCBI

37 

Grossi I, Schiavone M, Cannone E, Grejdan OA, Tobia C, Bonomini F, Rezzani R, Salvi A and De Petro G: Lasp1 expression is implicated in embryonic development of zebrafish. Genes (Basel). 14:352022. View Article : Google Scholar : PubMed/NCBI

38 

Vo TTL, Nguyen TN, Nguyen TT, Pham ATD, Vuong DL, Ta VT and Ho VS: SHOX2 methylation in Vietnamese patients with lung cancer. Mol Biol Rep. 49:3413–3421. 2022. View Article : Google Scholar : PubMed/NCBI

39 

Yu M, Carter KT, Makar KW, Vickers K, Ulrich CM, Schoen RE, Brenner D, Markowitz SD and Grady WM: MethyLight droplet digital PCR for detection and absolute quantification of infrequently methylated alleles. Epigenetics. 10:803–809. 2015. View Article : Google Scholar : PubMed/NCBI

40 

de Vos L, Jung M, Koerber RM, Bawden EG, Holderried TAW, Dietrich J, Bootz F, Brossart P, Kristiansen G and Dietrich D: Treatment response monitoring in patients with advanced malignancies using cell-free SHOX2 and SEPT9 DNA methylation in blood: An observational prospective study. J Mol Diagn. 22:920–933. 2020. View Article : Google Scholar : PubMed/NCBI

41 

Bergheim J, Semaan A, Gevensleben H, Groening S, Knoblich A, Dietrich J, Weber J, Kalff JC, Bootz F, Kristiansen G and Dietrich D: Potential of quantitative SEPT9 and SHOX2 methylation in plasmatic circulating cell-free DNA as auxiliary staging parameter in colorectal cancer: A prospective observational cohort study. Br J Cancer. 118:1217–1228. 2018. View Article : Google Scholar : PubMed/NCBI

42 

Crowley E, Di Nicolantonio F, Loupakis F and Bardelli A: Liquid biopsy: Monitoring cancer-genetics in the blood. Nat Rev Clin Oncol. 10:472–484. 2013. View Article : Google Scholar : PubMed/NCBI

43 

Elazezy M and Joosse SA: Techniques of using circulating tumor DNA as a liquid biopsy component in cancer management. Comput Struct Biotechnol J. 16:370–378. 2018. View Article : Google Scholar : PubMed/NCBI

44 

Stejskal P, Goodarzi H, Srovnal J, Hajdúch M, van't Veer LJ and Magbanua MJM: Circulating tumor nucleic acids: biology, release mechanisms, and clinical relevance. Mol Cancer. 22:152023. View Article : Google Scholar : PubMed/NCBI

45 

Postel M, Roosen A, Laurent-Puig P, Taly V and Wang-Renault SF: Droplet-based digital PCR and next generation sequencing for monitoring circulating tumor DNA: A cancer diagnostic perspective. Expert Rev Mol Diagn. 18:7–17. 2018. View Article : Google Scholar : PubMed/NCBI

46 

Luo H, Wei W, Ye Z, Zheng J and Xu RH: Liquid biopsy of methylation biomarkers in cell-free DNA. Trends Mol Med. 27:482–500. 2021. View Article : Google Scholar : PubMed/NCBI

47 

Krausewitz P, Kluemper N, Richter AP, Büttner T, Kristiansen G, Ritter M and Ellinger J: Early dynamics of quantitative SEPT9 and SHOX2 methylation in circulating cell-free plasma DNA during prostate biopsy for prostate cancer diagnosis. Cancers (Basel). 14:43552022. View Article : Google Scholar : PubMed/NCBI

48 

Huang X, Duijf PHG, Sriram S, Perera G, Vasani S, Kenny L, Leo P and Punyadeera C: Circulating tumour DNA alterations: Emerging biomarker in head and neck squamous cell carcinoma. J Biomed Sci. 30:652023. View Article : Google Scholar : PubMed/NCBI

49 

Beltrán-García J, Osca-Verdegal R, Mena-Mollá S and García-Giménez JL: Epigenetic IVD tests for personalized precision medicine in cancer. Front Genet. 10:6212019. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Grossi I, Assoni C, Lorini L, Smussi D, Gurizzan C, Grisanti S, Paderno A, Mattavelli D, Piazza C, Pelisenco IA, Pelisenco IA, et al: Evaluation of DNA methylation levels of <em>SEPT9</em> and <em>SHOX2</em> in plasma of patients with head and neck squamous cell carcinoma using droplet digital PCR. Oncol Rep 51: 52, 2024.
APA
Grossi, I., Assoni, C., Lorini, L., Smussi, D., Gurizzan, C., Grisanti, S. ... Bossi, P. (2024). Evaluation of DNA methylation levels of <em>SEPT9</em> and <em>SHOX2</em> in plasma of patients with head and neck squamous cell carcinoma using droplet digital PCR. Oncology Reports, 51, 52. https://doi.org/10.3892/or.2024.8711
MLA
Grossi, I., Assoni, C., Lorini, L., Smussi, D., Gurizzan, C., Grisanti, S., Paderno, A., Mattavelli, D., Piazza, C., Pelisenco, I. A., De Petro, G., Salvi, A., Bossi, P."Evaluation of DNA methylation levels of <em>SEPT9</em> and <em>SHOX2</em> in plasma of patients with head and neck squamous cell carcinoma using droplet digital PCR". Oncology Reports 51.3 (2024): 52.
Chicago
Grossi, I., Assoni, C., Lorini, L., Smussi, D., Gurizzan, C., Grisanti, S., Paderno, A., Mattavelli, D., Piazza, C., Pelisenco, I. A., De Petro, G., Salvi, A., Bossi, P."Evaluation of DNA methylation levels of <em>SEPT9</em> and <em>SHOX2</em> in plasma of patients with head and neck squamous cell carcinoma using droplet digital PCR". Oncology Reports 51, no. 3 (2024): 52. https://doi.org/10.3892/or.2024.8711
Copy and paste a formatted citation
x
Spandidos Publications style
Grossi I, Assoni C, Lorini L, Smussi D, Gurizzan C, Grisanti S, Paderno A, Mattavelli D, Piazza C, Pelisenco IA, Pelisenco IA, et al: Evaluation of DNA methylation levels of <em>SEPT9</em> and <em>SHOX2</em> in plasma of patients with head and neck squamous cell carcinoma using droplet digital PCR. Oncol Rep 51: 52, 2024.
APA
Grossi, I., Assoni, C., Lorini, L., Smussi, D., Gurizzan, C., Grisanti, S. ... Bossi, P. (2024). Evaluation of DNA methylation levels of <em>SEPT9</em> and <em>SHOX2</em> in plasma of patients with head and neck squamous cell carcinoma using droplet digital PCR. Oncology Reports, 51, 52. https://doi.org/10.3892/or.2024.8711
MLA
Grossi, I., Assoni, C., Lorini, L., Smussi, D., Gurizzan, C., Grisanti, S., Paderno, A., Mattavelli, D., Piazza, C., Pelisenco, I. A., De Petro, G., Salvi, A., Bossi, P."Evaluation of DNA methylation levels of <em>SEPT9</em> and <em>SHOX2</em> in plasma of patients with head and neck squamous cell carcinoma using droplet digital PCR". Oncology Reports 51.3 (2024): 52.
Chicago
Grossi, I., Assoni, C., Lorini, L., Smussi, D., Gurizzan, C., Grisanti, S., Paderno, A., Mattavelli, D., Piazza, C., Pelisenco, I. A., De Petro, G., Salvi, A., Bossi, P."Evaluation of DNA methylation levels of <em>SEPT9</em> and <em>SHOX2</em> in plasma of patients with head and neck squamous cell carcinoma using droplet digital PCR". Oncology Reports 51, no. 3 (2024): 52. https://doi.org/10.3892/or.2024.8711
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team