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Zinc finger protein 180 induces an apoptotic phenotype by activating METTL14 transcriptional activity in colorectal cancer

  • Authors:
    • Liang Xu
    • Xi-Jie Chen
    • Qian Yan
    • Xin-Tao Lei
    • Hai-Ling Liu
    • Jing-Ping Xu
    • Wei-Te Shang
    • Jing-Lin Huang
    • Zhi-Ting Chen
    • Xiao-Li Tan
    • Han-Jie Lin
    • Xin-Hui Fu
    • Li-Sheng Zheng
    • Ping Lan
    • Yan Huang
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, The Sixth Affiliated Hospital, Sun Yat‑sen University, Guangzhou, Guangdong 510655, P.R. China, Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, Guangdong Institute of Gastroenterology, The Sixth Affiliated Hospital, Sun Yat‑sen University, Guangzhou, Guangdong 510655, P.R. China, Department of Pathology, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong 510080, P.R. China
    Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 125
    |
    Published online on: July 25, 2024
       https://doi.org/10.3892/or.2024.8784
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Abstract

Zinc finger protein 180 (ZNF180) is a multifunctional protein that interacts with nucleic acids and regulates various cellular processes; however, the function of ZNF180 in colorectal cancer (CRC) remains unclear. The present study investigated the role and function of ZNF180 in CRC, and aimed to reveal the underlying molecular mechanism. The results revealed that ZNF180 was downregulated in CRC tissues and was associated with a good prognosis in patients with CRC. Additionally, the expression of ZNF180 was downregulated by methylation in CRC. In vivo and in vitro experiments revealed that ZNF180 overexpression was functionally associated with the inhibition of cell proliferation and the induction of apoptosis. Mechanistically, chromatin immunoprecipitation‑PCR and luciferase assays demonstrated that ZNF180 markedly regulated the transcriptional activity of methyltransferase 14, N6‑adenosine‑methyltransferase non‑catalytic subunit (METTL14) by directly binding to and activating its promoter region. Simultaneous overexpression of ZNF180 and knockdown of METTL14 indicated that the reduction of METTL14 could suppress the effects of ZNF180 on the induction of apoptosis. Clinically, the present study observed a significant positive correlation between ZNF180 and METTL14 expression levels, and low expression of ZNF180 and METTL14 predicted a poor prognosis in CRC. Overall, these findings revealed a novel mechanism by which the ZNF180/METTL14 axis may modulate apoptosis and cell proliferation in CRC. This evidence suggests that this axis may serve as a prognostic biomarker and therapeutic target in patients with CRC.
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Copy and paste a formatted citation
Spandidos Publications style
Xu L, Chen X, Yan Q, Lei X, Liu H, Xu J, Shang W, Huang J, Chen Z, Tan X, Tan X, et al: Zinc finger protein 180 induces an apoptotic phenotype by activating METTL14 transcriptional activity in colorectal cancer. Oncol Rep 52: 125, 2024.
APA
Xu, L., Chen, X., Yan, Q., Lei, X., Liu, H., Xu, J. ... Huang, Y. (2024). Zinc finger protein 180 induces an apoptotic phenotype by activating METTL14 transcriptional activity in colorectal cancer. Oncology Reports, 52, 125. https://doi.org/10.3892/or.2024.8784
MLA
Xu, L., Chen, X., Yan, Q., Lei, X., Liu, H., Xu, J., Shang, W., Huang, J., Chen, Z., Tan, X., Lin, H., Fu, X., Zheng, L., Lan, P., Huang, Y."Zinc finger protein 180 induces an apoptotic phenotype by activating METTL14 transcriptional activity in colorectal cancer". Oncology Reports 52.4 (2024): 125.
Chicago
Xu, L., Chen, X., Yan, Q., Lei, X., Liu, H., Xu, J., Shang, W., Huang, J., Chen, Z., Tan, X., Lin, H., Fu, X., Zheng, L., Lan, P., Huang, Y."Zinc finger protein 180 induces an apoptotic phenotype by activating METTL14 transcriptional activity in colorectal cancer". Oncology Reports 52, no. 4 (2024): 125. https://doi.org/10.3892/or.2024.8784
Copy and paste a formatted citation
x
Spandidos Publications style
Xu L, Chen X, Yan Q, Lei X, Liu H, Xu J, Shang W, Huang J, Chen Z, Tan X, Tan X, et al: Zinc finger protein 180 induces an apoptotic phenotype by activating METTL14 transcriptional activity in colorectal cancer. Oncol Rep 52: 125, 2024.
APA
Xu, L., Chen, X., Yan, Q., Lei, X., Liu, H., Xu, J. ... Huang, Y. (2024). Zinc finger protein 180 induces an apoptotic phenotype by activating METTL14 transcriptional activity in colorectal cancer. Oncology Reports, 52, 125. https://doi.org/10.3892/or.2024.8784
MLA
Xu, L., Chen, X., Yan, Q., Lei, X., Liu, H., Xu, J., Shang, W., Huang, J., Chen, Z., Tan, X., Lin, H., Fu, X., Zheng, L., Lan, P., Huang, Y."Zinc finger protein 180 induces an apoptotic phenotype by activating METTL14 transcriptional activity in colorectal cancer". Oncology Reports 52.4 (2024): 125.
Chicago
Xu, L., Chen, X., Yan, Q., Lei, X., Liu, H., Xu, J., Shang, W., Huang, J., Chen, Z., Tan, X., Lin, H., Fu, X., Zheng, L., Lan, P., Huang, Y."Zinc finger protein 180 induces an apoptotic phenotype by activating METTL14 transcriptional activity in colorectal cancer". Oncology Reports 52, no. 4 (2024): 125. https://doi.org/10.3892/or.2024.8784
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