SNX10 regulates the proliferation, apoptosis and cell cycle of acute B lymphoblastic leukemia cells via the PI3K/Akt signaling pathway

Wang,Chenyu; Yang,Xiulan; Shen,Xue; Yan,Shirong; Li,Jing; Wang,Yan; Tao,Tian; Wu,Tongqian; Kang,Qian; Yu,Fang

doi:10.3892/or.2025.8911

SNX10 regulates the proliferation, apoptosis and cell cycle of acute B lymphoblastic leukemia cells via the PI3K/Akt signaling pathway

Authors:
- Chenyu Wang
- Xiulan Yang
- Xue Shen
- Shirong Yan
- Jing Li
- Yan Wang
- Tian Tao
- Tongqian Wu
- Qian Kang
- Fang Yu
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Affiliations: Center for Clinical Laboratories, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou 550004, P.R. China, Department of Clinical Microbiology and Immunology, School of Clinical Laboratory Science, Guizhou Medical University, Guiyang, Guizhou 550004, P.R. China, Clinical Research Center, Affiliated Hospital of Guizhou Medical University, Guiyang Guizhou 550004, P.R. China, Department of Hematology, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou 550004, P.R. China
Published online on: May 8, 2025 https://doi.org/10.3892/or.2025.8911
Article Number: 78
Copyright : © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY 4.0].

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Abstract

B‑cell acute lymphoblastic leukemia (B‑ALL) is a type of acute lymphoblastic leukemia that originates from B cells. It typically occurs in children and adolescents, but it can also appear in adults. Sorting nexin 10 (SNX10) has recently been identified as a significant regulatory factor in various tumors, although its specific roles remain contested. However, its function in B‑ALL has not been previously explored. The present study investigated the role of SNX10 in B‑ALL pathogenesis. Bioinformatics analysis identified SNX10 as a Core Hub gene in the B‑ALL signaling network, with significantly reduced expression in patients with B‑ALL. These findings were corroborated through analysis of clinical bone marrow samples and B‑ALL cell lines. Functional in vitro studies revealed that SNX10 knockdown markedly inhibited B‑ALL cell proliferation, increased apoptosis, and arrested cells in the G0/G1 phase. By contrast, SNX10 overexpression enhanced cell proliferation, suppressed apoptosis and promoted G2/M phase progression. Proteomic analysis further implicated the PI3K/Akt signaling pathway in mediating the effects of SNX10. Specifically, SNX10 overexpression increased the phosphorylation levels of PI3K and Akt, while SNX10 knockdown had the opposite effect. In vivo experiments demonstrated that elevated SNX10 expression accelerated leukemia progression in a mouse model. Collectively, these findings highlighted the pivotal role of SNX10 in promoting B‑ALL cell proliferation via the PI3K pathway, highlighting its potential as a therapeutic target for B‑ALL and providing a foundation for future investigations.

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