Isolation of a cDNA for the human homolog of MAT-1 oncogene and its expression in human breast cancer cells
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- Published online on: January 1, 1996 https://doi.org/10.3892/or.3.1.137
- Pages: 137-140
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Abstract
Overexpression of mouse MAT-1 oncogene is associated with carcinogensis of mouse mammary epithelial cells. A human cDNA (hMAT) homologous to MAT-1 was isolated from normal human breast tissue. The hMAT cDNA is 1312 bp long and produces a 8.5 kDa peptide in vitro. The hMAT probe hybridized with 2.5 kb RNA in normal human breast epithelial cells from reduction mammoplasty specimens and in human breast cancer cell lines. The extent of hMAT gene expression in human breast cancer cell line was variable, with BT-20, T47-D, and MDA-MB-231 showing about a 10-fold overexpression compared to primary normal human breast epithelial cells, MCF-7, and ZR-75-1.
