Basic mutant Max reverses a c-Myc block to differentiation

  • Authors:
    • C Cultraro
    • T Cogliati
    • L Hearing
    • S Segal
  • View Affiliations

  • Published online on: January 1, 1996     https://doi.org/10.3892/or.3.1.141
  • Pages: 141-146
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Abstract

Murine erythroleukemia (MEL) cells overexpressing a transfected c-myc gene are blocked in their ability to undergo inducer-mediated differentiation, whereas overexpression of a transfected max gene mutated within the basic region (bm-max) accelerates differentiation. Based on these findings, we cotransfected MEL cells with plasmids which express human c-Myc constitutively and bm-Max in a zinc-inducible manner. Competition of endogenous proteins for binding to bm-Max can be considered negligible in cells expressing such high constitutive levels of c-Myc. Thus, this system provides a cell culture model for studying Myc:Max complex formation and its effect on erythroid differentiation. Clones expressing high levels of c-Myc and low levels of bm-Max are blocked in their ability to undergo N,N'-hexamethylene bisacetamide (HMBA)-mediated differentiation, presumably due to a preponderance of growth-promoting Myc:Max complexes. However, increased expression of bm-Max, in these clones, allows differentiation to occur by decreasing the levels of functional Myc:Max complexes. Although the exogenously expressed c-Myc and bm-Max associate in vivo, the basic region mutation in bm-Max abolishes the binding of Myc:bm-Max complexes to the specific E-box consensus sequence. We demonstrate that this sequestering of c-Myc by bm-Max reverses the c-Myc block to differentiation.

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January 1996
Volume 3 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Cultraro C, Cogliati T, Hearing L and Segal S: Basic mutant Max reverses a c-Myc block to differentiation. Oncol Rep 3: 141-146, 1996
APA
Cultraro, C., Cogliati, T., Hearing, L., & Segal, S. (1996). Basic mutant Max reverses a c-Myc block to differentiation. Oncology Reports, 3, 141-146. https://doi.org/10.3892/or.3.1.141
MLA
Cultraro, C., Cogliati, T., Hearing, L., Segal, S."Basic mutant Max reverses a c-Myc block to differentiation". Oncology Reports 3.1 (1996): 141-146.
Chicago
Cultraro, C., Cogliati, T., Hearing, L., Segal, S."Basic mutant Max reverses a c-Myc block to differentiation". Oncology Reports 3, no. 1 (1996): 141-146. https://doi.org/10.3892/or.3.1.141