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Detection of p53 mutations in gynecologic tumors by means of a simple, nonradioactive minigel method of PCR-SSCP analysis

  • Authors:
    • N Martinez
    • E Jimenez
    • J Castresana
    • J Schneider

  • View Affiliations

  • Published online on: March 1, 1997     https://doi.org/10.3892/or.4.2.359
  • Pages: 359-361
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Abstract

We studied 22 gynecological tumors for mutations in exons 5 through 8 of the p53 gene by means of SSCP analysis, using a simplified, nonradioactive technique developed at our laboratory. In order to test the procedure, we carried it out in parallel with the usual radioactive one. Two coincident mutations (in exons 5 and 8) were found by means of both methods. However, an additional one in exon 6 was found by means of our nonradioactive technique. This simplified method for SSCP-analysis of the p53 gene in human tumors is faster, easier and cheaper to perform than the conventional radioactive method, and yields equivalent results. It could be thus an excellent candidate for routine use in the clinical setting.

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March 1997
Volume 4 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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  • Journal Metrics
  • Impact Factor: 4.2
  • Ranked Oncology
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  • CiteScore: 8.4
  • CiteScore Rank: Q1: #68/366 Oncology
  • Source Normalized Impact per Paper (SNIP): 0.786
  • SCImago Journal Rank (SJR): 0.812
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Spandidos Publications style
Martinez N, Jimenez E, Castresana J and Schneider J: Detection of p53 mutations in gynecologic tumors by means of a simple, nonradioactive minigel method of PCR-SSCP analysis. Oncol Rep 4: 359-361, 1997
APA
Martinez, N., Jimenez, E., Castresana, J., & Schneider, J. (1997). Detection of p53 mutations in gynecologic tumors by means of a simple, nonradioactive minigel method of PCR-SSCP analysis. Oncology Reports, 4, 359-361. https://doi.org/10.3892/or.4.2.359
MLA
Martinez, N., Jimenez, E., Castresana, J., Schneider, J."Detection of p53 mutations in gynecologic tumors by means of a simple, nonradioactive minigel method of PCR-SSCP analysis". Oncology Reports 4.2 (1997): 359-361.
Chicago
Martinez, N., Jimenez, E., Castresana, J., Schneider, J."Detection of p53 mutations in gynecologic tumors by means of a simple, nonradioactive minigel method of PCR-SSCP analysis". Oncology Reports 4, no. 2 (1997): 359-361. https://doi.org/10.3892/or.4.2.359