Induction of p53 protein by carbon-ion and proton beam irradiation in two close human lymphoblastoid cell lines with different p53 status

Fukushima,S; Ozeki,S; Tang,J; Koizumi,M; Matsumura,S; Inoue,T; Yamazaki,H; Murayama,S; Hatanaka,K; Ejiri,H; Kasai,K

doi:10.3892/or.4.3.481

Induction of p53 protein by carbon-ion and proton beam irradiation in two close human lymphoblastoid cell lines with different p53 status

Authors:
- S Fukushima
- S Ozeki
- J Tang
- M Koizumi
- S Matsumura
- T Inoue
- H Yamazaki
- S Murayama
- K Hatanaka
- H Ejiri
- K Kasai
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Affiliations: OSAKA UNIV,GENOME INFORMAT RES CTR,SUITA,OSAKA 565,JAPAN. OSAKA UNIV,SCH DENT,DEPT ORAL & MAXILLOFACIAL RADIOL,SUITA,OSAKA 565,JAPAN. HOSP UNIV PENN,UNIV PENN,SCH MED,DEPT RADIAT ONCOL,PHILADELPHIA,PA 19104. NATL CANC CTR,DIV RADIAT THERAPY,CHUO KU,TOKYO 104,JAPAN. OSAKA UNIV,NUCL PHYS RES CTR,IBARAKI,OSAKA 567,JAPAN. NATL INST RADIOL SCI,INAGE KU,CHIBA 263,JAPAN.
Published online on: May 1, 1997 https://doi.org/10.3892/or.4.3.481
Pages: 481-484

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Abstract

In this study, we compared the kinetics of the postirradiation p53 protein expression for carbon ion beam (290 MeV/n, LET 75 keV/mu m) and proton beam (65 MeV) with that of (13)7Cs-gamma ray. We used two human lymphoblastoid cell lines derived from the same donor with different p53 status. Wild-type p53 protein increased after irradiation and it was dose-dependent. Meanwhile, the mutated p53 protein level did not show any increase with irradiation. With the three forms of radiation, there was no significant difference as regards the p53 protein kinetics.