Detection of hepatitis C virus in sera and genotyping according to the 5' non-coding region

  • Authors:
    • M Tsopanomichalou
    • M Ergazaki
    • S Linardopoulos
    • E Kouroumalis
    • D Spandidos
  • View Affiliations

  • Published online on: November 1, 1997     https://doi.org/10.3892/or.4.6.1171
  • Pages: 1171-1174
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Abstract

A reverse transcription (RT)-polymerase chain reaction (PCR) method was used for detection of the RNA of hepatitis C virus (HCV) in 120 samples of sera from Crete, which were positive for HCV-specific antibodies, by ELISA and Western blot analyses. A segment of 255 bp, located in the most conserved region of the HCV genome (the 5' untranslated region, 5' UTR), was amplified. For the identification of sequence variation from the HCV-1 strain, twenty of these samples were sequenced and compared to prototype strain (HCV-1) according to current genotypic classification. We were able to identify fourteen of the twenty as type 1a (i.e. similar to the prototype), two as type 1b, two as type 3a and two as type 4a. These findings generally agree with the geographic distribution of the already identified genotypes, though 3a type has not been reported previously in Crete (Greece).

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November 1997
Volume 4 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Tsopanomichalou M, Ergazaki M, Linardopoulos S, Kouroumalis E and Spandidos D: Detection of hepatitis C virus in sera and genotyping according to the 5' non-coding region. Oncol Rep 4: 1171-1174, 1997
APA
Tsopanomichalou, M., Ergazaki, M., Linardopoulos, S., Kouroumalis, E., & Spandidos, D. (1997). Detection of hepatitis C virus in sera and genotyping according to the 5' non-coding region. Oncology Reports, 4, 1171-1174. https://doi.org/10.3892/or.4.6.1171
MLA
Tsopanomichalou, M., Ergazaki, M., Linardopoulos, S., Kouroumalis, E., Spandidos, D."Detection of hepatitis C virus in sera and genotyping according to the 5' non-coding region". Oncology Reports 4.6 (1997): 1171-1174.
Chicago
Tsopanomichalou, M., Ergazaki, M., Linardopoulos, S., Kouroumalis, E., Spandidos, D."Detection of hepatitis C virus in sera and genotyping according to the 5' non-coding region". Oncology Reports 4, no. 6 (1997): 1171-1174. https://doi.org/10.3892/or.4.6.1171